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Structure of maculae densa is observed around renal corpuscle, but no medullary loop is discovered.

肾小囊是肾小管的起始端,由内、外两层壁层构成,内层与肾小球的毛细血管紧贴,外层为单层扁平上皮细胞。

Tongue, roof of mouth, gums and flews --solid bluish-black is preferred in all coat colors except in dilute colors, which have a solid lavender pigmentation.

舌头、口腔内上半部、牙龈和上唇,兰黑色是首选;浅色沙皮犬的上述部位应该是淡紫色。

Results 8 days after intracutaneous injection,fully developed pigmented hair follicle formed.2 weeks later,skin surface of injection site became black and evlevated, a grey sac formed lined with keratinized epithelium,lots of hair follicles formed with black hair shafts and fully developed sebaceous glands.

结果皮内移植后8天可见有完整的黑色毛囊形成,2周见移植区皮肤呈黑色隆起,移植物形成一个灰白色囊,内衬角化上皮,周围见大量新生毛囊及黑色毛干,新生毛囊呈向心性排列,伴发育良好的皮脂腺。

Methods To use Ce3+ or Pb2+ as capture agents to detect carbonic anhydrase, Na+-K+-ATPase, Mg2+-ATPase, 5' –nucleotidase and NADPH oxidase activities on endosome membrane in epithelial cells of renal proximal tubules、hepatic cell of rat and human leukocytes. with cytochemical method.

用细胞化学方法,以Ce3+或Pb2+作捕捉剂,分别检测碳酸酐酶,Mg2+-ATP酶,Na+- K+-ATP酶,5'-核等酸酶,NADPH oxidase在大鼠肾近曲小管上皮细胞、肝细胞以及人体嗜中性白细胞内吞体膜上的酶活性。

FGFR4 expression was faint in renal vesicle and primitive tubules of S-shaped body, irrecognizable in urteric bud and podocyte of C-stage, negative in mesenchyme and condensing mesenchyme.

胚胎肾发生带内FGFR4表达微弱,肾囊泡和S型小体的上支和中间支,即原始肾小管上皮细胞部位有微弱表达,间充质、压缩间充质未见表达,输尿管芽及其末端壶腹和C-期足细胞表达不明显。

Light microscope and transmission electron microscopy showed that SMMC-7721 cells induced by SAHA had undergone the restorational alteration in morphology and ultrastructure, which were different from those of nontreated cells but were similar to those of normal cells, and the changes were as follows: the cells turned to be flat and spread; the nucleo-cytoplasmic ratio lessened and nuclear shape became rather regular; the number of nucleolus reduced and its volume lessened; euchromatin increased while heterochromatin decreased in nucleus; in the cytoplasm, mitochondria grew in number with relatively consistent structure and well-developed mitochondria cristae; Golgi complex turned to be well-developed and typical; rough endoplasmic reticulum increased. Immunocytochemistry assay showed that the expression of AFP and PCNA were declined significantly. FCM analysis showed SAHA could arrest SMMC-7721 cells in G0/G1 phase, with an accumulation of the cells in G0/G1 phase while a decrease of cells in S phase. Semi-quantitative RT-PCR detection revealed that the expression of p21WAFl mRNA was upregulated remarkably in the cells treated with SAHA.

结果:倒置显微镜和透射电镜观察显示,经SAHA处理的细胞增殖速度显著减慢,细胞体积增大,细胞核较小,形状较为规则,核仁数量减少、体积变小,核内常染色质增多而异染色质减少,核质比例减小,细胞质内线粒体数量增多、线粒体嵴发达,高尔基体较为典型,粗糙型内质网增多,呈现出与正常上皮细胞相似的形态变化;MTT比色法测定结果显示不同浓度(2.5、5.0、7.5、10.0uM)SAHA对SMMC-7721细胞的增殖均有抑制作用,并有明显的剂量依赖和时间依赖关系;免疫细胞化学检测显示SAHA能显著降低PCNA和AFP在SMMC-7721细胞中的表达;流式细胞仪检测结果显示,SMMC-7721细胞经SAHA处理后,G0/G1期细胞明显增加,S期细胞则明显减少,细胞被阻滞于G0/G1期;RT-PCR检测结果表明,SAHA作用12h后SMMC-7721细胞中p21WAF1 mRNA的表达即有增加,24h后更为明显。

After observing the specimens from JMA-m groups, we have found that epithelium and endothelium of cornea full, the lesion are light edema at stromatic layer. One small amount of inflammatory-cell infiltrate presents the collagen fiber lack of rules at shallow stromatic layer.

观察角膜安的结果表明,中药中剂量组角膜上皮和内皮层完整,基质轻度水肿,浅基质层内少量炎性细胞浸润,胶原纤维走行欠规则,整体改变较低剂量组减轻。

The results showed:(1) The histological changes of KC were in fact a wound-repair process;(2) The increased melanin in KC coordinated with "multi-overlapping screen" effect, a result of multiple layers of "supranuclear cap", was the direct cause of hyperpigmentation of the graft and (3) The hydrolytic disturbance of melanin in KC and the longer life span of the KC were probably the important reasons for the increase of melanin in the epidermis.

结果表明,角朊细胞的组织学改变实际上是一个损伤后修复的过程;角朊细胞内黑色素含量增加及出现的多层次的&核上帽&状分布而形成的&多重帘幕&效应,是皮片过度色素化的直接原因;黑色素在角朊细胞内的降解障碍及角朊细胞更新周期延长可能是表皮内黑色素含量增多的重要原因。

RESULTS:In BN rat retina,Cx43 was immunolocalized to internal limiting membrane,nerve fiber layer,ganglion cell layer and pigment epithelium.Cx43 appeared in the form of distinct puncta distributed on ganglion cell.

结果:正常视网膜内界膜,视神经纤维层以及神经节细胞层明显表达,神经节细胞表达的主要位置是细胞质和细胞膜,色素上皮细胞全层表达,内外颗粒层及内外层状层无表达。

Histologically, the seminiferous tubules contained numerous Sertoli cells and more Sertoli-spermatozoa complexes, accompanied by the depletion of Leydig cells with deeply stained nuclei. Mature spermatozoa were stored up in the epididymis, but only a few in the efferent ducts. In the second place was testicular atrophy(32/120; 26.7%). The seminiferous tubules showed moderate to severe inactivity of spermiogenesis with evidence of only spermatogonia, spermatocytes and Sertoli cells. The Leydig cells were obviously decreased in numbers associated with decrease of lipid droplets in their cytoplasms. Testicular hypoplasia was the third disorders(22/120; 18.3%). Only a few spermatogonia and Sertoli cells appeared without any spermiogenesis. The associated changes was decreased in Leydig cells and fibrous hyperplasia in the interstitium.Epididymal stones were sometimes found(12/120; 10%). Grossly, yellowish-white nodules with various sizes and firm in consistency were observed in the epididymis and the front efferent ducts. Microscopically, the epididymal ducts were dilated with voluminous spermatozoa storage, even showed calcification in severe cases. The deposited calcium salts were stained positively by Von Kossa and Alizarin red methods.Amyloidosis was also detected in 10 roosters(8.3%). Eosinophilic, homogeneous, amyloid-like substances were deposited mainly in the testicular interstitium and the periphery of blood vessels. These substances showed positive reaction by Congo red staining. Five roosters(4.2%)had Marek's lesions in the testis, epididymis and peripheral nerves with infiltration of pleomorphic lymphocytes. Only one case showed epithelial necrosis of seminiferous tubules accompanied by fibrous proliferation in the interstitium.

结果发现,在总共搜集的120个病例中,其中因年老所导致的产精力不佳为最多,占38例(31.7%),於镜下可见大量精虫黏附於Sertoli cell的表面,并可见Sertoli cell数量明显增多而Leydig cell明显减少,且其细胞核呈现浓染的现象,而在其副睪中仍可见到成熟精虫蓄留於管腔中,但在其输精管内却只有少量精虫存在;其次为睪丸萎缩,占32例(26.7%),镜下可见中度至重度无造精作用,其生精小管中只见到精母细胞、精原细胞及Sertoli cell存在,但Leydig cell数量明显减少且其细胞质内的脂质也明显减少;睪丸发育不全,占22例(18.3%),於生精小管内只见到精母细胞及少量Sertoli cell存在,不见造精细胞分化,於生精小管间质可见Leydig cell减少并伴随结缔组织增生;副睪结石,占12例(10%),肉眼下可在副睪及输精管前段见到黄白色大小不一的结节,触感坚硬,於镜下可见副睪管扩张并有大量成熟精虫蓄积,严重时可见钙化现象,以Von Kossa及茜素红染色均呈阳性反应;类淀粉沉著症,占10例(8.3%),镜下在睪丸间质及血管周围可见粉红均质样的物质沉积,以刚果红染色成阳性反应;马立克病,占5例(4.2%),镜下可在睪丸、副睪实质及周边神经内均可见到嗜碱性大小不一的淋巴样细胞浸润;睪丸坏死,占1例(0.8%),镜下可见生精小管上皮细胞坏死脱落及间质结缔组织增生。

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