上皮内的

Between P14 and P21,CIAPIN1 immunoreaction in the brain,heart and liverbecame much lower. However,between P21 and P28,CIAPIN1 immunoreactionin the heart,brain,liver and skeletal muscle became much lower,while with thekidney development,CIAPIN1 immunoreaction in the kidney became higher. Invarious tissues from adult mouse,CIAPIN1 immunoreaction could be seen incardiac muscle cell,brain,hepatocyte,epithelium of renal tubule,skeletal muscle,lung tissue,gastric mucosa and gland,acinus lienalis.2. Distribution of CIAPIN1 in normal fetal and adult human tissuesTo reveal the possible physiological role of CIAPIN1,we examined theexpression and distribution of CIAPIN1 in fetal and adult human tissues usingimmunohistochemistry. We found that CIAPIN1 was ubiquitously distributed infetal and adult tissues,and was localized in both the cytoplasm and the nucleus.

然而，在3个月大的成年鼠中，CIAPIN1阳性反应物在心、脑、肝和肾小管中的表达强度要低于P28小鼠；但CIAPIN1阳性反应物在成年鼠骨骼肌中较P28小鼠高。2、CIAPIN1蛋白在人5个月胚胎及成人多器官组织内的表达在人5月胚胎多器官组织中，CIAPIN1阳性反应物见于心脏、胆囊单层柱状上皮和粘膜、结肠粘膜、小肠粘膜和绒毛、肝脏、直肠腺体、胃粘膜、肾上腺束状带、甲状腺滤泡、脾索、胸腺小叶间隔、皮肤真皮层和汗腺、睾丸白膜和间质、脑组织内神经元和神经胶质、肺小支气管和肺泡、骨骼肌、肾脏皮髓质和肾小管、子宫内膜、胰腺腺泡和胰岛、卵巢、输卵管粘膜等绝大多数组织细胞。

RESULTS: The expression of VCAM1 in the lung tissues of normal rats was weak, and distributed mainly in alveolar epithelial cells, bronchiole epithelial cells, alveolar macrophage and some pulmonary interstitium.

结果： 在正常对照组，VCAM1大鼠肺组织中呈弱表达，分布于肺泡上皮细胞、细支气管上皮细胞、肺泡巨噬细胞及少量的肺内间质。

In this study, we found that β-catenin, c-JUN, c-myc and GSK3β might be involved in squamous metaplasia of airway epithelium induced by methylcholanthrene, vitamin A deficiency or cigarette smoking by investigating the rat model of squamous metaplasia.

本项目通过维生素A缺乏、吸烟及气管内注入甲基胆蒽复制体外气道上皮鳞化动物模型，发现β-连环素、c-JUN、c-myc 及GSK3β可能介导了甲基胆蒽、VitA 缺乏与吸烟所致的大鼠支气管上皮鳞状化生。

RESULT: 1.Ouabain act on lens sodium-pump,under the LM,lens anterior capsular membrane discontinuous,epithelium cells clustered,occluding zonule seperated,lens fiber layers fractured.Under the EM,cells totally hollowed,mitochondria swelling,myelin figure appeared.RT-PCR examine the expression condition of αsubunit of sodium pump on mRNA level,α1、α2 and α3-isoform are all decreased.2.Digoxin act on lens sodium-pump,under the LM,lens cell oedema,linkage distructed,extensive exfoliation.Under the EM,plasma appeared little half-transparant hollow region,mitochondria swelling and ridge disappeared. RT-PCR examine,α1、α2 and α3-isoform are all decreased.3.Amphotericin B act on lens sodium-pump,under the LM,lens epithelium cells linked tightly,arranged in-line,lens fiber layers arranged tightly and regularily.Under the EM,abbundant cellular organes,exuberant cells function indicated. RT-PCR examine the expression condition of αsubunit of sodium pump on mRNA level,α1 and α3-isoform are increased significantly,demonstrated isoform-specific action.4D-thyroxine act on lens sodium-pump,under the LM,lens plasmalemma integrated,cells arranged tightly and regularily.Under the EM,nucleus fission appeared,desmosome half-desmosome and tensile microfilaments linked the cells. RT-PCR examine,α2 and α3-isoform are increased, also demonstrated isoform-specific action.5.Vitamin E act on lens sodium-pump,under the LM,lens anterior capsular membrane continuous and smooth,epithelium cells tightly linked,lens fiber layers appearede hollow region occasionally.Under the EM,lateral membrane high density belt appeared,abundant nucleolus. RT-PCR examine,onlyα1-isoform are increased, demonstrated significantly isoform-specific action.6.DMSO act on lens sodium-pump,under the LM,lens anterior capsular membrane slightly thicker,cells linkage partly distructed.Under the EM,plasmalemma denaturation,mitochondria swelling.RT-PCR examine,α1、α2 and α3-isoform are all altered slightly and haven't significant meanning.

结果：1、哇巴因作用于晶状体钠泵后，光镜下晶状体前囊膜断裂、上皮细胞聚积、闭合连接分离、纤维板层裂隙，电镜下全层细胞空泡化、线粒体肿胀出现髓样结构，RT-PCR法检测晶状体钠泵α亚单位α1、α2及α3三种重整异构体在mRNA水平的表达均减弱。2、地高辛作用于晶状体钠泵后，光镜下晶状体细胞水肿、细胞连接破坏、广泛剥离，电镜下胞质见少许半透明空化区、线粒体肿胀嵴消失，RT-PCR法检测晶状体钠泵α亚单位α1、α2及α3三种重整异构体在mRNA水平的表达均减弱。3、两性霉素B作用于晶状体钠泵后，光镜下晶状体上皮细胞紧密连接、线状排列、纤维板层紧密规整，电镜下细胞器丰富、细胞功能旺盛，RT-PCR法检测α1及α3表达显著增强、具有一定的重整异构作用特异性。4、D甲状腺素作用于晶状体钠泵后，光镜下晶状体质膜完整、细胞排列紧密规整，电镜下胞核见分裂像、细胞间有桥粒、半桥粒及张力微丝，RT-PCR法检测α2及α3表达均增强、亦有一定的重整异构作用特异性。5、维生素E作用于晶状体钠泵后，光镜下晶状体前囊膜连续光滑、上皮细胞紧密连接、纤维板层偶见空化，电镜下囊侧膜内有高电子密度带、细胞核仁丰富，RT-PCR法检测仅有α1的表达显著增强、具有极强的重整异构作用特异性。6、二甲基亚砜作用于晶状体钠泵后，光镜下晶状体前囊膜轻度增厚、细胞连接部分破坏，电镜下质膜变性、线粒体肿胀，RT-PCR法检测晶状体钠泵α亚单位α1、α2及α3三种重整异构体在mRNA水平的表达无显著改变。

The stable clones are further identified by RT-PCR and Western blot; 6 MTT assay is used to investigate the effect of ZNRD1 on the cell growth of cells (AGS, SGC7901, MKN28, NIH3T3, GES-1); 7 Soft agar assay is used to investigate the effect of ZNRD1 on the clonality of cells (AGS, MKN28); 8 Nude mice assay is used to investigate the effect of ZNRD1 on the cell growth of gastric cancer cells (AGS, MKN28); 9 Flow cytometry is used to investigate the effect of ZNRD1 on the cell cycle distribution of cells (AGS, MKN28, NIH3T3, GES-1); 10 Flow cytometry is used to investigate the effect of ZNRD1 on the cell apoptosis of cells (AGS, MKN28, NIH3T3); 11 MTT assay is used to investigate the effect of ZNRD1 on the drug sensitivity of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR) in vitro; 12 SRCA is used to investigate the effect of ZNRD1 on the drug sensitivity of gastric cancer cells (SGC7901, SGC7901/VCR) in vivo; 13 Flow cytometry is used to investigate the effect of ZNRD1 on adriamycin accumulation of cancer cells (SGC7901, SGC7901/VCR, HL-60, HL-60/VCR); 14 Transmission electron microscope is used to investigate the effect of ZNRD1 on the sensitivity of SGC7901 cells towards drug-induced apoptosis; 15 Flow cytometry and DNA ladder assay are used to investigate the effect of ZNRD1 on the sensitivity of cells (SGC7901, SGC7901/VCR, HL-60/VCR) towards drug-induced apoptosis; 16 Microarray is used to investigate the profiling of ZNRD1-responsive genes in gastric cancer cells (AGS, MKN28, SGC7901, SGC7901/VCR); 17 RT-PCR and Western blot are used to identify the results of microarray; 18 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of cyclin D1; 19 Reporter gene assay is used to investigate the effect of ZNRD1 on the transcriptional activity of MDR1; 20 Kinase assay is used to investigate the effect of ZNRD1 on the activity of cyclin E-CDK2 kinase; 21 The antisensenucleic acids of p21 is used to inhibit the expression of p21, and flow cytometry is used to investigate the effect of p21 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 22 The antisensenucleic acids of p27 is used to inhibit the expression of p27, and flow cytometry is used to investigate the effect of p27 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 23 Liposome is used to up-regulate the expression of Skp2, and flow cytometry is used to investigate the effect of Skp2 on ZNRD1-induced cell cycle arrest in gastric cancer cells; 24 Western blot is used to investigate the effect of ZNRD1 on the stability of Skp2 and p27 in gastric cancer cells; 25 MVD assay is used to investigate the effect of ZNRD1 on the angiopoietic activity of gastric cancer cells; 26 ELISA is used to investigate the effect of ZNRD1 on the expression of VEGF165 in gastric cancer cells; 27 The roles of DARPP-32 in MDR of gastric cancer cells are investigated using gene transfection, MTT assay, SRCA, flow cytometry and DNA ladder assay.

应用杂交瘤技术制备ZNRD1的首个单克隆抗体；2）利用RT-PCR、Western blot和免疫组化检测ZNRD1在胃癌组织、胃炎组织、正常胃上皮组织、胃癌细胞和正常胃组织上皮细胞中的表达；3）构建ZNRD1的小干扰RNA载体，并测序鉴定；4）利用脂质体将ZNRD1的真核表达载体及其空载体转染胃癌细胞（AGS、SGC7901、MKN28）和小鼠成纤维细胞（NIH3T3），G418筛选后进行鉴定；5）利用脂质体将ZNRD1的小干扰RNA载体及其空载体转染药敏胃癌细胞（SGC7901）、正常胃组织上皮细胞（GES-1）、对长春新碱耐药的胃癌细胞（SGC7901/VCR）、药敏白血病细胞（HL-60）、对长春新碱耐药的白血病细胞（HL-60/VCR），G418筛选后进行鉴定；6）利用MTT实验检测ZNRD1高/低表达对细胞（AGS、SGC7901、MKN28、NIH3T3、GES-1）生长的影响；7）通过软琼脂克隆形成实验检测上调ZNRD1对AGS、MKN28细胞克隆形成能力的影响；8）通过裸鼠成瘤实验检测上调ZNRD1对AGS、MKN28细胞体内成瘤性的影响；9）通过流式细胞仪分析ZNRD1高/低表达对细胞（AGS、MKN28、NIH3T3、GES-1）的细胞周期的影响；10）通过流式细胞仪分析上调ZNRD1对细胞（AGS、MKN28、NIH3T3）的凋亡的影响；11）通过MTT实验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）体外药物敏感性的影响；12）通过肾包膜下移植法检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR）体内药物敏感性的影响；13）通过流式细胞仪分析ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60、HL-60/VCR）内阿霉素蓄积和泵出的影响；14）通过透射电镜检测上调ZNRD1对SGC7901细胞凋亡敏感性的影响；15）通过流式细胞仪和DNA梯度试验检测ZNRD1高/低表达对细胞（SGC7901、SGC7901/VCR、HL-60）凋亡敏感性的影响；16）通过基因芯片检测ZNRD1高/低表达对胃癌细胞内基因表达谱的影响；17）利用RT-PCR、Western blot对基因芯片的结果进行鉴定；18）利用报告基因实验检测ZNRD1对cyclin D1的启动子活性的调节作用；19）利用报告基因实验检测ZNRD1高/低表达对MDR1的启动子活性的调节作用；20）利用激酶试验检测ZNRD1对cyclin E-CDK2 激酶活力的影响；21）利用反义核酸技术抑制p21的表达；通过流式细胞仪检测抑制p21对ZNRD1介导的细胞周期阻滞的影响；22）利用反义核酸技术抑制p27的表达；通过流式细胞仪检测抑制p27对ZNRD1介导的细胞周期阻滞的影响；23）利用脂质体转染法上调Skp2的表达；通过流式细胞仪检测上调Skp2对ZNRD1介导的细胞周期阻滞的影响；24）利用Western blot检测ZNRD1对p27和Skp2的蛋白稳定性的影响；25）利用微血管密度实验检测ZNRD1对AGS、MKN28细胞裸鼠移植瘤微血管形成的影响；26）利用ELISA检测ZNRD1对AGS、MKN28细胞培养上清和移植瘤匀浆中VEGF165含量的影响；27）利用脂质体转染法、MTT实验、肾包膜下移植法、流式细胞仪和DNA梯度试验检测新耐药相关分子DARPP-32对细胞（SGC7901、SGC7901/VCR、对阿霉素耐药的胃癌细胞SGC7901/ADR）多药耐药表型的影响；利用脂质体转染法和MTT实验检测下调ZNRD1对DARPP-32介导的胃癌多药耐药的调控作用。

To reveal chemokines mediating the celiac metastasis of epithelial ovarian carcinoma by way of investigating the chemokine receptor expressions in serous epithelial ovarian carcinoma cell lines and tissues, and the corresponding ligand expressions in membrana abdominis.

本研究通过分析浆液性上皮性卵巢癌细胞系和组织趋化因子受体的表达，及腹膜间皮细胞相应配体的表达，以揭示趋化因子介导上皮性卵巢癌腹腔内转移机制。

The two-photon absorption cross-sections of these styryl derivatives were determined by Z-scan technique, and the benzothiazolium derivatives exhibited the largest TPA cross-sections (10〓s·cm〓/photon) and the best power limiting ability at high operable incident intensity. The results show that the benzothiazolium derivatives are promising organic compounds as two-photon absorption optical limiting materials. 2. Several organic dyes active to 800nm femtosecond laser with D-π-A structure were designed and synthesized.

研究了它们的上转换荧光、上转换激射和光限幅行为，通过改变染料分子共轭体系的大小，首次实现了在576nm-635nm范围内的双光子上转换激光波长的调节，其中噻唑啉碘盐类二苯乙烯化合物的双光子上转换激射峰（576nm）是迄今在1064nm皮秒激光作用下能够得到的最短波长的激光；阴离子显著改变上转换激射效率，但对上转换荧光峰和激射峰的位置影响很小；增加溶液的浓度，上转换荧光光谱和激射光谱红移，并在一定的浓度范围内，能够有效的提高上转换激射效率；利用Z-扫描技术测定了染料分子的双光子吸收截面，发现苯并噻唑类染料分子的双光子吸收截面高达10〓s·cm〓／photon，而且在较大的入射光强范围内，这类染料分子具有较大的光限幅能力，是一类作为双光子吸收光限幅材料很有潜力的染料分子。

Some exudation and bleedings happen in the pulmonary alveoli, some inflammatory cells infiltrate into lung tissues; in the serum AMY, ALP, TNF-α, IL-6 increases obviously high, COX-2 expression increase; The expression of NF-κB in big rat lung of SAP increase, and the nucleus is thick dyed, it explain that the NF-κ moved into the nucleus; Stimulate the inflamatory reaction.

我们的实验中见到SAP组的大鼠肺脏组织细胞腔扩大、肺泡壁断裂，有的出现肺大泡，肺泡内伴有渗出及少量非出血，肺组织可见咽细胞浸润；血清中AMY、ALP、TNF-α、IL-6明显增高；在SAP组大鼠肝细胞、肾小管上皮细胞、肺脏小气管及肺泡上皮上COX-2表达增加；NF-κB在SAP大鼠肺脏中表达增加，并且出现核浓染，说明NF-κB前移入核；启动炎症反应等一系列反应SAP大鼠受损伤的肺组织中AQP-1无论在mRNA水平上、蛋白水平上还是免疫组化染色病理学检测上都明显下降。

Vitrectomy allows us to manage otherwise untreatable ocular pathologies, but the substitution of the vitreous by an artificial intraocular irrigation solution would probably disturb the physiology of the retina and choroids.

中文题名眼内灌注液对视网膜色素上皮细胞凋亡的影响副题名外文题名 The effects of different intraocular irrigating solutions on the apoptosis of RPE cells 论文作者殷春悦导师黎晓新教授学科专业眼科学研究领域\研究方向临床医疗技能训练与研究学位级别博士学位授予单位北京大学学位授予日期2001 论文页码总数94页关键词玻璃体切除术眼科手术细胞凋亡视网膜色素上皮细胞眼内灌注液馆藏号BSLW /2001 /R779.6 /42 随着玻璃体切除术的出现和日益发展，眼内灌注液作为术中使用的玻璃体替代物，直接影响视网膜和脉络膜的正常生理功能。

TGF-β1 took on extensive positive in alveolus epithlia in the control group. The positive cytes of the groups under hyperxia-exposure for 3, 7, 14 day distributed in alveolar epithelium, pulmonary interstitial, bronchoepithlia and macrophage, which were notably enhanced along with the time of hyperxia-exposure.

IFN-γmRNA在高氧3天（0.62+0.49）、7天（0.44+0.53）、14天（0.39+0.27 ）均高于空气组(0.20+0.14)，高氧3天达到高峰水平（P.05），高氧7天后开始下降（P＞0.05），高氧14天下降更为明显（P＞0.05）；TGF-β1的阳性细胞在空气组广泛分布于肺泡上皮，高氧组各期广泛分布于肺泡上皮、肺内间质、支气管上皮、血管内皮和巨噬细胞。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐