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We got the result: Bufo Raddei Strauch Tadpole (1) Bufo Raddei Strauch Tadpole was capable of lens regeneration, which originated from the epithelial cells at iris dorsal margin by dipigmentation.(2) Dipigmentation of Bufo Raddei Strauch by two methods: one was licked up by amoebocyte, the other one was flowed from the pigment cells.(3) In the lens regeneration cells of Bufo Raddei Strauch profiles of the mitochondria became complicated ribosome manifold, and the endoplasmic reticulum was cisternal dilation. During the process of deferentiation of lens fiber the cell nucleolus became little and chromatin shrinked.(4)The crystalline electrophoresis of Bufo Raddei Strauch Tadpole lens regeneration showed that during lens regeneration P-crystalline was expressed earliest then was γ-crystalline and α-crystallin, this result is consistent with newt.

结果表明:花背蟾蜍蝌蚪(1)具有晶状体再生的能力,其晶状体的再生来源于虹膜背缘的上皮细胞;(2)虹膜背缘去色素是转分化的前提,主要有两种方式:一种也是最主要的方式是在晶状体被摘除后虹膜外侧产生巨噬细胞,巨噬细胞吞噬虹膜色素上皮细胞中的色素颗粒;另一种方式是虹膜色素上皮细胞自身释放色素颗粒;(3)电镜观察发现虹膜色素上皮细胞转分化过程中线粒体有明显变化,核糖体增多,粗面内质网增多,晶状体纤维分化过程中细胞核逐渐凝聚;(4)SDS-PAGE电泳发现,花背蟾蜍蝌蚪晶状体再生中相关蛋白表达顺序与有尾类蝾螈相似,蛋白表达顺序依次是β-晶状体蛋白、γ-晶状体蛋白和α-晶状体蛋白,并且后两种蛋白几乎同时表达。

The results of the TEM showed the same respiration epithelium in the inside gill and the gill filament, it is flat, the cytoplast of the epithelium contained of the rough endoplasmic reticulum, vacuole and mitochondrion in rich; the blood-gas barrier is formed of the monolayer epithelium, the endothelial cell and the basilar membrane between the both, following the development the blood-gas barrier attenuate gradually.

电镜结果表明花背蟾蜍外鳃与内鳃具有相同的呼吸上皮,为扁平状,上皮细胞质中富含粗面内质网及空泡和线粒体;气血屏障由由单层上皮细胞,内皮细胞以及二者之间的基底膜构成,随着个体的发育,气血屏障逐渐变薄。

Mu opioid receptor was not observed in the wrist, funnel, abdomen of mantle, mouth, craw, stomach theca, intestine, rectum, sialoid gland, liver. Weak positive mu opioid receptor immunoreaction was found in inner epidermis, ectoblast, connective tissue of the esophagus and the keratolytic of the stomach and positive immunoreaction in epidermis of back of mantle and membrane of wrist.

结果表明,短蛸腕、漏斗、外套膜腹面、口球、嗉囊、胃盲囊、肠、直肠、前唾液腺、后唾液腺、肝胰脏均呈μ受体阴性,但食道内上皮、外膜、结缔组织和胃角质层有μ受体分布,外套膜背面、腕间膜的上皮或近上皮部位呈μ受体阳性。

Results Over 90% of nasal and sinus cavity got clean within 1 to 2 weeks postoperatively. About 80% of patients had the De-mucous reaction from the 3rd to 10th week , such as mucous edema, vesicles, granulation tissue, mini-polyps, fibrous hyperplasia, and adhesion or regenerated diseases may appear in this stage. All of these diseases competed with mucous epithelization.

结果 90%以上的术腔在1~2周内清洁,80%以上的术腔在3~10周内有水肿、囊泡、肉芽、息肉生长和纤维结缔组织增生、粘连等去粘膜化反应或再生病变发生,并与上皮化呈竞争性生长。90%的术腔在经过恰当的处理后可完成上皮化,其中接近60%的术腔是在术后11~14周完成上皮化。

Probably the intestinal barrier function correlates with decreasing [Ca2+] of the Intestinal epithelial cells to lessen the intestinal epithelial cells injury and decreasing the release of histaminase in the Intestinal epithelial cells.(4) Fufangdanshen mixture can enhance effects of Dachengqi herbs in treating incomplete intestinal obstruction.

4活血承气合剂肠屏障保护作用可能与抗自由基及降低小肠粘膜上皮细胞内钙离子超载,从而减轻小肠粘膜上皮细胞损伤,降低小肠粘膜上皮细胞组胺酶的释放有关。

Methods Porcine bile duct and ureter were treated using detergent sodium dodecylsulphate and 1% Triton X-100 to prepare the acellular lumens matrix. The toxic effects of different concentrations of acellular lumens matrix extract were tested by MTT to assess the proliferation of human scarfskin fibroblasts. The cytotoxicity of the target biomaterial was graded according to the national standards. The growth manner of the human intrahepatic bile duct endothelial cells seeded on the acellular lumens matrix was studied after 20 d under scanning electron microscopy.

采用十二烷基磺酸钠、Triton X-100处理封闭群小香猪输尿管和胆管,获得去细胞管腔基质支架材料; MTT法检测基质支架材料的不同浓度浸提液对细胞增殖的影响,并按医用生物材料细胞毒性分级标准进行评级;在基质支架材料上种植人肝内胆管上皮细胞,于接种后20 d通过扫描电镜观察人肝内胆管上皮细胞在支架上的生长情况。

The results shown that:(1) Bufo raddei Strauch tadpole was capable of lens regeneration, which originated from the epithelial cells at dorsal iris margin by depigmentation.(2) Depigmentation of Bufo raddei Strauch during lens regeneration was by two methods: First, pigment granules initially dispersed all over the cytoplasm were moved towards the periphery of the cell, and then were directly taken up by the amoeboid cells. Second, pigment granules in a cell were first crowded in a mass, surrounded by a membranous structure in the cytoplasm, which were eventually discharged as a mass from the cell.

结果表明:花背蟾蜍蝌蚪(1)具有晶状体再生的能力,其晶状体的再生来源于虹膜背缘的上皮细胞;(2)虹膜背缘去色素是转分化的前提,主要有两种方式:一种也是最主要的方式是在晶状体被摘除后虹膜外侧产生巨噬细胞,巨噬细胞吞噬虹膜色素上皮细胞中的色素颗粒;另一种方式是虹膜色素上皮细胞自身释放色素颗粒;(3)电镜观察发现虹膜色素上皮细胞转分化过程中线粒体有明显变化,核糖体增多,粗面内质网增多,晶状体纤维分化过程中细胞核逐渐凝聚。

Xenopus laevis Tadpole (1) Xenopus laevis Tadpole was capable of lens regeneration, which originated from the outer cornea.(2)The days of lens regeneration of Xenopus laevis Tadpole was earlier than Bufo Raddei Strauch Tadpole, The rate of regeneration of Xenopus laevis Tadpole was higher than Bufo Raddei Strauch Tadpole.(3)During Xenopus laevis lens regeneration the outer cornea cells proliferated, in the elongating cells the mitochondria became complicated and in the columnar cells the cisternal dilating of endoplasmic reticulum was ubiquity.

非洲爪蟾蝌蚪(1)再生晶状体来源于外角膜;(2)晶状体再生较花背蟾蜍蝌蚪晶状体再生时期早,再生率高;(3)电镜观察发现再生前期外角膜增殖,观察到晶状体上皮细胞和伸长细胞(进一步发育为晶状体纤维细胞),伸长细胞中线粒体形状各异随着进一步的分化,细胞器逐渐消失;分化过程中晶状体上皮细胞粗面内质网增多,并且内质网扩张普遍存在。

The location and structures of sex-pheromone-producing gland in female H.insularis were studied by EAG,GC,SEM,and TEM.These studies showed that thegland situate in the intersegmental membrane between the eighth and ninthabdominal segments,and is an eversible abdominal fold;Many plump cones disturbon the surface of the gland.The glandular cells of 2-day old virgin female H.insularis are arranged in one layer,among which the central cells are columnarepithelial cells and flat on two sides.The nucleus is irregular elliptical.There isevident conjugation between cells and the involution is more in the basal membraneof cell.Microvilli are distributed on the cytoplasmic membrane and linked withendocuticle on which there are many layers of chitin,and the outer cuticule is staineddeeper.The cell contains bubbles,mitochondria,glycogen deposits,roughendoplasmic reticulum and smooth endoplasmic reticulum.

结合触角电位、毛细管气相色谱、扫描电镜、透视电镜等技术对小线角木蠹蛾雌蛾腹尖末端不同组织部位提取物的测定分析以及腺体位置和形态结构的观察发现:小线角木蠹蛾性信息素分泌腺位于腹部末端8~9节之间,是一个由节间膜特化而成的上皮结构,为一可外翻的腹褶,腺体表面分布着饱满的锥形体,羽化后2天未交尾的雌蛾腺体细胞呈单层排列,腹面中央由密集的柱形上皮细胞组成,细胞排列向两侧延伸至背部,其形状由柱形逐渐变为扁平形,细胞核为椭圆形,细胞与细胞间有明显的胞连接,细胞基底膜基褶较多,质膜上分布着微绒毛,并与内表皮连接,内表皮上有多层几丁质,外角质层染色较深,细胞质中含有空泡、线粒体、脂质粒、粗面内质网和光面内质网。

The results are unsure for experimental use;The rabbit's corneas that were removed with upper-half of corneal limbal epithelium lamella and erased the center corneal epitheliums were transparent with intact corneal epithelium;In the approach,the corneal and limbal epitheliums were burned with a cotton swab socked in 1 mol/L NaOH,there were 4 rabbits' corneal stroma happened perforation or ulcer and symblepharon,and the other one presented corneal epithelium phenotype.This is an applicable method to create the pathological model of corneal limbal stem cell total deficiency.

结果表明,处理后4周,全周角膜缘上皮板层手术切除,中央角膜上皮层用1 mol/L NaOH擦除的5只试验家兔角膜表面全部血管化、结膜化,未发生睑球粘连,角膜基质胶原纤维完整未见溃疡、穿孔等病变,细胞印迹学检查为结膜表型,可作为实验性角膜缘干细胞移植的病理模型;全周角膜缘上皮板层手术切除,中央角膜上皮用生理盐水擦除的5只试验家兔,有2只为结膜表型,另3只为角膜表型,观察期内结果不稳定;半周角膜缘上皮板层手术切除,中央角膜上皮层用生理盐水擦除的5只试验家兔,角膜表面透明,全部为角膜表型;直接用1 mol/L NaOH擦除角膜缘和中央角膜上皮的试验家兔,有4只角膜基质胶原纤维断裂、溶解,并伴有严重的溃疡、穿孔、睑球粘连等病变,不能用于移植试验,另1只角膜表面透明,未见结膜和新生血管长入,细胞印迹学检查为角膜表型。

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