上皮下的

Prometheus prometheus was atitan.in the war between zeus the giants he had stood on the side of the new olympiangods.out of the clay he made the first man,to whom athena gave soul and holy breath.prometheus spent alot of time and energy in creating the gift of fire.and fire raised man above all animals.later,there held ajoint meeting of gods and men.the meeting was to decide what part of burnt animals should begiven to gods and what to men.prometheus cut up an ox and divided it into two parts:under the skin he placed the fresh,and under the fat he put the bones,for he knew the selfish zeus loved fat.zeus saw through the trick and felt displeased at the prometheus\'favor towards men.so in amasterful way he took away the gift of fire from mankind.however,prometheus managed to steal fire from heaven and secretly brought it down tomen.flying into an anger at t你好s unjustified act of rebellion,zeus let the other gods chain prometheus to arock on mountain caucasus,where ahungry eagle ever tore at 你好s liver w你好ch ever grew again.你好s period of pain was to be t你好rty-thousand years.prometheus faced 你好s bitter fate firmly and never lost courage before zeus.at last heracles made prometheus and zeus restore to friend s你好p,when heracles came over in search of the golden apple and killed the eagle and set the friend of mankind free.

普罗米修斯是泰坦伟人之一在宙斯与伟人的战役中，他站在新的奥林波斯山神一边他用粘土造出了第一个男人雅典娜付与了这个男人魂灵和极其崇高的生命普罗米修斯还花费了很多时间和精力创造了火，并将之赠予人的总称火使人成为万物之灵在这之后，进行了第一次神与人的联席会议这个会议将决定烧烤过的动物的哪一部分该分给神，哪一部分该给人的总称普罗米修斯切开一头牛，把它分成两部分：他把肉放在皮下，将骨头放在肥肉下因为他懂得自私的宙斯爱吃肥肉宙斯看破了他的把戏普罗米修斯偏袒人的总称，这使宙斯感到烦懑因此，他专横地把火从人的总称手中夺走然而，普罗米修斯设法窃走了天火，偷偷地把它带给人的总称宙斯对于他这类肆无忌惮的背抗行为大发雷霆他令其他的山神把普罗米修斯用锁链缚在高加索山脉的一块片岩上一只饥饿的老鹰天天来啄食他的肝脏，而他的肝脏又老是重新长出来他的痛苦要连续三万年而他坚定地面对于魔难，从来不在宙斯面前丧失勇气最后，海格立斯使普罗米修斯与宙斯恢复了他们的友情，找到了金苹果，杀死了老鹰，故而解救了人的总称的老朋友

Methods: Eighty healthy adult SD rats were respectively implanted with MSCs/β-TCP complex in left thigh and β-TCP in right thigh. According to different treating methods, the materials were enveloped with, or transfixed into the supereme genual periosteal flap, or enveloped with superficial epigastric deep fascia flap, or embedded into subcutaneous thigh.

80只成年SD大鼠，根据植入材料不同分2组，即左侧的复合MSCs的β-TCP生物陶瓷和右侧的单纯β-TCP生物陶瓷，再按不同的处理因素分为带膝最上血管骨膜支的骨膜瓣包裹组和贯穿组，带腹壁浅血管的深筋膜包裹组和直接股部皮下埋放组。

The PSCA_3 fragment was selected for its superior expression level in eukaryotic cells.Then the sig-PSCA_3-Fc-GPI genetic fragment was cloned into pVAX1-neo-IRES-GM/B7 vector to construct the final immunological inhanced DNA vaccine pVAX1-PSCA_3-FcGB. Immunofluorescence and flow cytometry were used to confirm the expression of PSCA_3 fragment by transfected into Cos7 cell.Finally,the anti-tumor effect of pVAX1-PSCA_3-FcGB was tested in murine prostate cancer model generated by RM-1 cell line.The animal was immunized with pVAX1-PSCA_3-FcGB DNA vaccine by intramuscular injection plus electroporation,pVAX1 and pVAX1-PSCA_1-FcGB plasmid were used as control.The inhibitory effect of tumor was investigated by observion of forming time,volume and inhibition ratio of tumor.Results:DNA sequencing conformed that the heterological PSCA fusion antigen fragment which was synchronized by overlapping-extending-PCR,was consistent to design.Enzyme digestion analysis showed that the 1 to 4 copies heterological PSCA fusion antigen fragments were constructed successfully.

方法(1)检索GenBank，选择包含人主要T细胞抗原表位序列的人PSCA基因片段，应用异种化抗原设计技术，保留人T细胞抗原表位，设计异种化PSCA融合抗原片段；(2)根据核酸序列按中心模板法设计引物，应用重叠延伸PCR技术拼接合成异种化PSCA融合抗原片段基因，以PCR、限制性酶切和DNA序列测定法进行鉴定：(3)利用DNA限制性内切酶BssHⅡ和MluⅠ酶切后粘端互补的特点，采用同尾酶法构建1—4拷贝异种化PSCA融合抗原片段(PNCA_1-PSCA_4)，并将上述片段分别插入真核表达载体pCI-neo-Fc-GPI中，转染293T细胞，借助免疫荧光+流式细胞术考察插入片段表达效率，最终选定PSCA_3片段进行下一步研究；(4)将sig-PSCA_3-Fc-GPI基因片段自pCI-PSCA_3-Fc-GPI质粒上切下，插入pVAX1-neo-IRES—GM/B7载体中，构建免疫增效DNA疫苗pVAX1-PSCA_3-FcGB，并应用转染Cos7细胞+免疫荧光/流式细胞术方法鉴定其在真核细胞中的表达情况；(5)给8周龄雄性C57BL/6小鼠皮下种植RM-1细胞，制备小鼠前列腺癌模型，并采用股四头肌肌肉注射+电脉冲法(Electroporation，EP)接种DNA疫苗质粒pVAX1-PSCA_3-FcGB，同时接种pVAX1空载体质粒和pVAX1-PSCA_1-FcGB质粒作为对照，通过观察计算免疫动物的成瘤时间、肿瘤体积和抑瘤率，来评价该DNA疫苗在小鼠体内的抑瘤效果。

The present study was carried out on paddy osils under conditions of continuous application of organic manures.We found that organic manuer applied could provide nutrients deficient in rhizosphere, enchanced the formation of chelated compounds of Fe and Mn in root horizon and play a significant role in decreasing the lignifcation of meahanical tissue and in increasing the stelar catheter diameter of roots.

采用有机肥与化肥配施，在田间小区试验和盆栽试验的基础上，研究了有机肥促进根层络合态Fe和Mn的形成，补偿根际养分亏缺，并对根的皮下机械组织木质化程度降低，中柱导管的增大有明显的作用。

Data from the national key research "A multicentre study of 2 types of domestic levonorgestrel subdermal implants" were analyzed to study the factors that may be associated with discontinuation of two types of levonorgestrel subdermal implants due to menstrual irregularities among Chinese users.

国内外有关皮下埋植剂的应用研究表明：它的有效性非常高，在使用的前两年几乎可以与输卵管结扎相媲美，但其可接受性在很大程度上受到了单纯孕激素引起的副反应的影响。

Calcium, blood calcium decreased, the brain caused by increased nerve excitability plant, resulting in baby night waking, night terrors, night irritability, sleep; baby should be given calcium and vitamin D and more sun: A, calcium: elemental calcium daily 200-300mg (1) Composite Calcium Granule: 2-3 packets a day (each contains calcium 100mg)(2) the activity of calcium granules: 4-6 packets per day (calcium per tablet 50mg) B, fill D : daily 400-800IU (1) concentrated cod liver oil drops: 2-4 drops per day (2) vitamin D2 candy: the daily 400-800IU (3) vitamin D3 injections: once a month, 20-300000 IU attention these objects must be a doctor's prescription, prescribed application. C, to the sun to vitamin D, ultraviolet radiation to the skin 7 - dehydrocholesterol into vitamin D3, to help calcium absorption, could be a day outdoors on the sun for half an hour each afternoon. 3, bile is a yellow pigment bilirubin, was it yellow staining and faeces, after bilirubin by oxidation into biliverdin, thus making green manure, the manure discharge encountered in diapers in the air oxygen, so that oxidation of bilirubin biliverdin, so that the surface of green manure.

缺钙、血钙降低，引起大脑植物性神经兴奋性增高，导致孩子夜醒、夜惊、夜间烦躁不安，睡不安稳；应给孩子补钙和维生素D并多晒太阳：A、补钙：每日元素钙200-300mg(1)复合钙冲剂：每日2-3包(每包含钙100mg)(2)活性钙冲剂：每日4-6包(每片含钙50mg)B、补D：每日400-800IU(1)浓缩鱼肝油滴剂：每日2-4滴(2)维生素D2糖丸：每日400-800IU(3)维生素D3针剂：每月一次，20-30万IU注意上述物均须医生处方，遵医嘱应用。C、向太阳要维生素D，紫外线要将皮下7-脱氢胆固醇转变成维生素D3，帮助钙的吸收，可每天户外上下午各晒半小时。3、胆汁中胆红素是一种黄色色素，被它染色及粪便呈黄色，胆红素经氧化过后变为胆绿素，从而使粪便呈绿色，排出的粪便在尿布中遇到空气中的氧，胆红素使氧化成胆绿素，使粪便的表面呈绿色。

It has been put forward that there exists single Ball point and double Ball points on the symmetrical connecting-rod curves of equilateral mechanisms.

从鲍尔点的形成原理出发，分析对称连杆曲线上鲍尔点的产生条件，提出等边机构的对称连杆曲线上有单鲍尔点和双鲍尔点。

The factory affiliated to the Group primarily manufactures multiple-purpose pincers, baking kits, knives, scissors, kitchenware, gardening tools and beauty care kits as well as other hardware tools, the annual production value of which reaches US$ 30 million dollars.

集团所属工厂主要生产多用钳、烤具、刀具、剪刀、厨具、花园工具、美容套等五金产品，年生产总值3000万美元，产品价廉物美、选料上乘、质量保证，深受国内外客户的青睐