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Cancer of the larynx and hypopharynx is one of the most common head and neck malignancy. Squamous cell carcinoma remains the most common pathologic type, accounting for more than 90% cases. Although therapeutic modality has improved gradually, there is no sign that the 5-year survival rate has improved during the past 20 years. The metastasis of tumor cells represents the primary source of clinical morbidity and mortality in the large majority of laryngeal and hypopharyngeal tumors.

中文摘要喉癌及下咽癌是常见的头颈部癌症之一,它的组织病理学诊断百分之九十以上为鳞状上皮细胞癌,虽然医学逐渐进步,但此疾病患者的存活率在最近20年内并没有增加的现象,对咽喉癌病患的治疗而言,是否出现淋巴结及远隔转移对预后具有决定性的影响。

Objective To observe the biological changes of primary human nasopharyngeal epithelial cells in the early stage of immortalization.

目的我们利用与鼻咽癌密切相关的EB病毒和TPA的协同作用,观察原代人胚鼻咽上皮细胞逃避老化期后其生物学特性的变化。

These results revealed that overexpression of c-Myc upregulated telomerase, which in turn resulted in immortalization of laryngeal squamous cells, and this mechanism existed not only in the initiation of laryngeal carcinogenesis but also in the whole process of cancer development.

这些研究表明,喉癌的发生可能是由于c-Myc的过度表达使端粒酶表达上调,从而使喉鳞状上皮细胞达到永生化,这一机制不仅存在于喉癌发生的早期,而且贯穿于喉癌的发展过程。

By immunochemistry staining, both epithelium marker and mesenchyme marker were positive, which made further support for diagnosis.

3免疫组织化学标记,出现上皮性抗体和间叶性抗体的同时表达,进一步支持诊断。

Rethods The expression of ER and PR in 60 cases of uterine cervix cancer,41 cases of CIN and 40 cases of chronic cervicitis was detected by immunochemistry.

用免疫组化方法对60例宫颈癌、41例宫颈上皮不典型增生及40例慢性宫颈炎组织的石蜡标本进行ER、PR检测。

It was the first time that using 〓 cell line and normal immunity inbreed line BalB/c mice as objects to construct the subcutaneous transplanting ovarian cancer model. Histopathological experiment proved that the transplanting tumor was undifferentiated cancer. The ovarian tumor marker 〓 and the epithelial tumor marker 〓 were positive in the transplanting tumors. According to these proofs we thought we had already succeeded in constructing animal tumor model.

本实验首次采用卵巢癌〓细胞系,以正常免疫力的雌性近交系BalB/c小鼠为对象,建立了卵巢癌皮下移植瘤模型,经病理组织学检查证实移植瘤为低分化癌,经免疫组化检测证实移植瘤组织中卵巢肿瘤标志物〓和上皮性肿瘤标志物〓表达阳性,据此我们认为动物肿瘤模型建立成功。

Results: Expression of PAI-1, TIMP-1 and FN in kidney of diabetic rats began to increase in the second week of diabetes; PAI-1 and TIMP-1 mainly expressed in intracytoplasm of renal tubular epithelial cells, while FN mainly expressed in renal tubule matrix and glomerului; Their expression showed a tendency of increasing along with the progression of diabetes.

结果:PAI-1、TIMP-1及FN表达从糖尿病2周起较正常对照组明显增多(P.01),PAI-1和TIMP-1阳性表达主要位于肾小管上皮细胞胞浆内,FN阳性表达主要位于肾小管间质和肾小球,且随着糖尿病进展有逐渐增加的趋势。

Our results are similar to those observed with probiotic strains and suggest that indole could be important in the intestinal epithelial cells response to gastrointestinal tract pathogens.

我们的研究结果与对益生菌菌株的研究结果类似,这表明吲哚在肠道上皮细胞对胃肠道病原体的反应中可能起着重要的作用。

The liver oval cell line WB-F344 derives from adult normal Fisher 344 male rats. It's a kind of liver infantine epithelial cells and has characteristics of liver stem cells. Many studies in vitro and in vivo imply that it has the role as precursor cells of hepatocacinoma, which indicates its function in hepatocarcinogenesis.

肝卵圆细胞株WB-F344来自正常成年同基因Fisher344雄性大鼠,是一种肝脏幼稚上皮细胞,具有肝脏干细胞的性质,体内、外的许多研究表明,它与肝癌的发生有一定的关系,具有肝癌前体细胞的作用。

The liver oval cell line WB-F344 derives from adult normal Fisher 344 malerats.It's a kind of liver infantine epithelial cells and has characteristics of liver stemcells.Many studies in vitro and in vivo imply that it has the role as precursor cells ofhepatocacinoma,which indicates its function in hepatocarcinogenesis.

肝卵圆细胞株WB-F344来自正常成年同基因Fisher344雄性大鼠,是一种肝脏幼稚上皮细胞,具有肝脏干细胞的性质,体内、外的许多研究表明,它与肝癌的发生有一定的关系,具有肝癌前体细胞的作用。

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