上电

He told me about an opening in the model shop as a model gluer trainee -- building and gluing models together that are seen by the public, everything from a Ronald McDonald that may be in a McDonald's or an Indiana Jones life-size model.

他知道我那时候不快乐，不喜欢我所在的环境，所以就告诉我，乐高公司有一个职缺，是负责替模型上胶的实习生。那些模型就像麦当劳叔叔差不多大，或者是跟电影印地安琼斯的真人版模型一样。

Single-voxel 1H-MRS was carried on the precentral gyri in 110 patients with ALS and 24 patients with lower motor neuron syndrome compared with 89 healthy controls.

虽然一些影像技术如MRI已用于ALS研究，但其上运动神经元受损的范围和程度仍难以用传统神经影像或电生理方法定量化测量。

The PSCA_3 fragment was selected for its superior expression level in eukaryotic cells.Then the sig-PSCA_3-Fc-GPI genetic fragment was cloned into pVAX1-neo-IRES-GM/B7 vector to construct the final immunological inhanced DNA vaccine pVAX1-PSCA_3-FcGB. Immunofluorescence and flow cytometry were used to confirm the expression of PSCA_3 fragment by transfected into Cos7 cell.Finally,the anti-tumor effect of pVAX1-PSCA_3-FcGB was tested in murine prostate cancer model generated by RM-1 cell line.The animal was immunized with pVAX1-PSCA_3-FcGB DNA vaccine by intramuscular injection plus electroporation,pVAX1 and pVAX1-PSCA_1-FcGB plasmid were used as control.The inhibitory effect of tumor was investigated by observion of forming time,volume and inhibition ratio of tumor.Results:DNA sequencing conformed that the heterological PSCA fusion antigen fragment which was synchronized by overlapping-extending-PCR,was consistent to design.Enzyme digestion analysis showed that the 1 to 4 copies heterological PSCA fusion antigen fragments were constructed successfully.

方法(1)检索GenBank，选择包含人主要T细胞抗原表位序列的人PSCA基因片段，应用异种化抗原设计技术，保留人T细胞抗原表位，设计异种化PSCA融合抗原片段；(2)根据核酸序列按中心模板法设计引物，应用重叠延伸PCR技术拼接合成异种化PSCA融合抗原片段基因，以PCR、限制性酶切和DNA序列测定法进行鉴定：(3)利用DNA限制性内切酶BssHⅡ和MluⅠ酶切后粘端互补的特点，采用同尾酶法构建1—4拷贝异种化PSCA融合抗原片段(PNCA_1-PSCA_4)，并将上述片段分别插入真核表达载体pCI-neo-Fc-GPI中，转染293T细胞，借助免疫荧光+流式细胞术考察插入片段表达效率，最终选定PSCA_3片段进行下一步研究；(4)将sig-PSCA_3-Fc-GPI基因片段自pCI-PSCA_3-Fc-GPI质粒上切下，插入pVAX1-neo-IRES—GM/B7载体中，构建免疫增效DNA疫苗pVAX1-PSCA_3-FcGB，并应用转染Cos7细胞+免疫荧光/流式细胞术方法鉴定其在真核细胞中的表达情况；(5)给8周龄雄性C57BL/6小鼠皮下种植RM-1细胞，制备小鼠前列腺癌模型，并采用股四头肌肌肉注射+电脉冲法(Electroporation，EP)接种DNA疫苗质粒pVAX1-PSCA_3-FcGB，同时接种pVAX1空载体质粒和pVAX1-PSCA_1-FcGB质粒作为对照，通过观察计算免疫动物的成瘤时间、肿瘤体积和抑瘤率，来评价该DNA疫苗在小鼠体内的抑瘤效果。

On the basis of the preparation of special-grade sodium hypophosphite by one-step method in the high-speed reactor,the produced sodium hypophosphite and the byproduct calcium phosphite were further converted to higher-purity hypophosphorous acid and phosphorous acid products by ionic membrane electrodialysis.

在作者开发的高速反应器一步法制专用级次磷酸钠的基础上进一步将生成的次磷酸钠和副产的亚磷酸氢钙用离子膜电渗析法制成纯度更高的次磷酸及亚磷酸产品，产品质量升级，回收利用了副产亚磷酸氢钙，并减少了原料烧碱的用量。

Based on selection of patients,electric coagulation,separation and cutting was used for intramural myoma,broad ligament myoma,cervical myoma and subserous myoma respectively.It suggests that laparoscopic hysteromyomectomy is safe and feasible with little blood loss and short recovery in treating myoma.

在选择好病例的基础上，于腹腔镜下行子宫肌瘤剔除术，对壁间肌瘤、阔韧带肌瘤、宫颈管肌瘤、浆膜下肌瘤分别采用电凝、分离、切割等术式。

Through adding biased voltage VGS to gate of the nc-Si/c-Si heterojunction MAGFET, the inequipotential potentials are compensated by adjusting equivalent resistance values of conducting channels.

通过在纳米硅/单晶硅异质结MAGFET栅极上外加偏置电压VGS，调整导电沟道等效电阻阻值进行不等位电势补偿。

Composed of long takes of indirect gazes and oppressively alienated temps morts (where an eclectic assembly of anonymous residents alternately stare out the window, smoke a cigarette, handle their rifle, voyeuristically peep, awkwardly flirt, become inebriated, and even mischievously set on fire laundry that has been hanging on a clothesline), the fragmented, collage-like portraits of the tenants are interstitially connected through the recurring image of the building's dimly lit hallways, a visual metaphor for a culture adrift and in transition - a conduit to an undefined destination.

塔尔齐名的东欧诗电影导演，他的大部分电影都没有对白，只用近乎静默的长镜头去探索人物内心的孤独和绝望，展现后共产主义的东欧社会巨变下人们精神世界的空虚，94年的《走廊》无疑是这种风格的代表作，其中的长镜头堪称能与同年那部赫赫有名的《撒旦探戈》相媲美。巴塔斯与俄罗斯的安德烈。索科洛夫，匈牙利的贝拉。塔尔共同被称为继承塔尔科夫斯基诗电影风格的当代重要导演，他们的作品或多或少都受到了塔尔科夫斯基的影响，无论是在电影艺术语言还是在作品的主题上，但三人之间又有所不同。

Isoelectric focusing electrophoresis, which is one of the most important technologies, is used broadly in characterization of protein composition.

等电聚焦电泳作为蛋白质组分分析的重要手段，被广泛的运用到对蛋白质性质的分析上。

This PH is termed isoelectric point, at which proteins show their minimum solubility.

这个PH称为等电点，蛋白质在这一点上溶解度最

You run a small sample of your fraction , now very reduced in volume and quite clear on an isoelectric focusing gel.

你在等电聚焦胶上，跑少量的非常透明的所得样品。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。