上电

To study the expression, purification and bioactivity of human augmenter of liver regeneration in Pichia Pastoris, the expression plasmid pPICZαA- ALR was constructed and transformed into P. Pastoris by the method of electroporation transformation. Induced with 0.5% methanol, the 30 kD protein in the culture supernatant of recombinant P. Pastoris was confirmed to be rhALR by SDS-PAGE and Western blot. Quantitative analysis showed that the target protein was in a level of 66% of the total protein of the culture supernatant, with a yield of 40mg/L.we had performed DEAE anion exchange chromatography two times with excessive and regular adsorption quantity consecutively, and then the rhALR above 95% purity and 52% protein recovery could be obtained by G75 molecular sieve chromatography at last step. The bioactivity assay of the purified product showed that rhALR could stimulate the proliferation of HepG2, SMMC-7721 and NIH-3T3 in vitro.

为在毕赤酵母中分泌表达人肝再生增强因子，以色谱法分离纯化后进行体外活性研究，构建表达载体pPICZαA- ALR，经电穿孔转入毕赤酵母中，用0.5％甲醇诱导表达；重组酵母培养上清经SDS-PAGE电泳和western blot鉴定后表明， rhALR以分子量为30kD的二聚体为主；定量分析结果表明，重组酵母培养上清中rhALR约占总蛋白的66％，表达量约为40mg/L；经DEAE柱和G75柱纯化后，获得的rhALR纯度大于95％，得率为52％；体外生物学活性实验表明，rhALR能明显促进HepG2、SMMC-7721和NIH-3T3细胞的增殖。

Another research topic is evaluated lightning induced voltages on nearby overhead-lines. Lightning is a transient, high-current discharge whose path length is measured in kilometers. During flash, neighbor electromagnetic field change violently, that would induce voltages on close-by metal.

另一研究课题为，非直击雷害造成邻近区域内架空线上的感应电压的探讨，雷击闪络可视为长距离的放电暂态现象，过程中会使得雷击周遭的电、磁场剧烈变动，将会在邻近的金属物件上感应出电压造成二次的伤害。

The invention relates to an amplifier circuit containing a high-frequency transistor (1) and having a circuit (2) for adjusting the operating point, which provides a base current for the high-frequency transistor (1) according to the voltage released at a resistor (3) which is connected in the collector ring of the high-frequency transistor (1). The circuit (2) has a differentiating unit (20), fed by the supply voltage, which compares the voltage released at the resistor (3) with a reference voltage.

带有高频晶体管(1)的放大器电路具有一个用于工作点调整的电路(2)，其依赖于在连接在高频晶体管(1)的集电极电路中的电阻(3)上下降的电压为高频晶体管(1)提供基极电流，电路(2)具有一个由供电电压馈电的差分节(20)，其把在电阻(3)上下降的电压与基准电压比较。

In traditional, the working probe naturally sustaining repeated bending actions and wear, absolutely needs a mechanism to prevent it from being damaged, caused by the change of the probe's geometry and the increase of the contact resistance. Therefore, in this study, we develop a CMOS-based chip in connection with the electroformed probes to detect each of their counterforce, contact resistance to evaluate if their contact force and coplanarity are controlled within safe range.

传统上，探针常因为弯曲作动和磨耗，使几何形状产生改变，进而电阻值提升，使过大的电流通过探针而烧毁，或因共面度不佳部分探针未能确实接触IC焊垫误判成断路，故晶片的制作重点在於监测探针卡上个别探针的回馈力量，当探针量测IC时，可进行即时探针反作用力、接触电阻的检测，进而了解探针卡工作时受力是否均匀、平坦度是否符合要求等性质。

Uses completely imports the thermostable nylon (PA66) raw material to add the fire protection textile fiber fine note to become again, has does not plate the electricity, artistic, thermostable and the antiseptic property is good, can when welds and the wave front welds the body had guaranteed that does not distort on machine the board not crookedly, quality assurance, reasonable price!

全部采用进口耐高温尼龙（PA66）原料再加防火纤维精注而成，具有不镀电、美观、耐高温及防腐性能好，能在焊接及过波锋焊时体保证在机板上不变形不歪斜，固定在PCB板上！质量保证，价格合理！

Normally closed solenoid valve, coil power, the GATE VALVES armature under the action of electromagnetic force driving the first Vice-valve valve Seti, the main valve on the valve cup of fluid flow out through the Vice-valve, reducing the role of the main valve pressure on the valve cup, when the main valve valve to reduce pressure on the cup to a certain value, the armature driving the main valve valve cup, and use pressure Ershi main valve valve cup opening, medium flow.

常闭电磁阀，线圈通电后，衔铁在电磁力作用下先带动副阀阀塞提起，主阀阀杯上的流体经副阀流走，减少了作用在主阀阀杯上的压力，当主阀阀杯上的压力减少到一定值时，衔铁带动主阀阀杯，并利用压差而使主阀阀杯开启，介质流通。

The rules of fluid rate with different technical parameters: the increase of electromagnetic force causes the increases of the fluid rates at S/L interface and free surface, the decrease of skin depth, the increase of electric current density which will make the fluid at free surface flowing faster, while it doesnt affect the fluid rate at S/L interface. When the height of melt increases, the both of fluid rates will increase too. The increase of the width-thickness ratio augments the fluid rates along the wider edges at S/L interface evidently, while the rates along the narrow edges will have little change, and the rate at free surface has little change too. The increase of overheat degree minishes the range of mush zone, while the fluid rates at S/L interface and free surface will increase evidently.

流速的大小随各种不同工艺因素的变化呈现出一定的变化规律：随电磁力的增大，固液界面以及自由表面处的流速都会增大；集肤层渗入深度变薄，电流密度增大，会导致自由表面上流动的加强，但对固液界面处的流动速度影响不大；熔区高度的增加，也会使固液界面及自由表面处的流动增强；宽厚比增大时，固液界面上沿宽面方向的流动显著增强，但窄面方向流速变化不大，同时自由表面上流速变化也较小；过热度及温度梯度的增大，使糊状区减小，导致固液界面及自由表面处的流速显著增大。

When scanning, from light transmitter beams shines on the bar code on the basis of the photoelectric detector from bar code on the binvalidation of the reflected light intensity to respond to that, when scanning a white spot sweep to the surface or in two in the space between the black line, the reflection of light intensity, high current output a detector; when the scan-to-black lines, reflected light, detector output UN-effectual, on the basis of narrow-striped width or length of time made in response to changes in light and shade with bar code and converted to a little different current signal, after a post to the bezze codec.

扫描时，从平发射器发不入的平束照在条码上时，平电检测器根据平束从条形码上不直射返来的平不不弱度息不入回答，当扫描平不背扫到白纸背上或处于两条暗线之间的空缺处时，不直射平不不弱，检测器输不入一个不小电流；当扫描至暗线条洋时，不直射平不弱，检测器输不入小电流，并根据暗线宽或广息不入工夫不幼短差别的反响，随着条形码明暗的改变变化为人小差别的电流暗记，经过放不小后运输到泽码器洋去。

Scanning electron microscope and atomic force microscope revealed that ruthenium oxide particles incorporated onto the nafion polymer film. RuO/Nf/GCE film modified electrode showed excellent electrocatalytic activity for the oxidation of dopamine and ascorbic acid.

本研究主要是使用循环伏安法使氧化钌稳定沉积在Nafion修饰电极上，形成氧化钌/Nafion 薄膜修饰电极，利用电化学石英晶体微天平观察其在电极上的成长情形，旋转环碟电极法则是用来研究其电催化反应，并用扫描式电子显微镜观察氧化钌结合在Nafion聚合薄膜上的表面现象。

What's more,there are abundant energy on the Mars:such as the wind energy is much more than that on the earth; the geothermal energy on the Mars;the methane fuel produced by carbon dioxide and hydrogen;the nuclear power generation with heavy hydrogen and so on.

火星上有丰富的能源。如风能比地球上要丰富得多；火星上有地热能；还可利用二氧化碳和氢制造甲烷燃料；也可用重氢进行核发电等等。

But we don't care about Battlegrounds.

但我们并不在乎沙场中的显露。

Ah! don't mention it, the butcher's shop is a horror.

啊！不用提了。提到肉，真是糟透了。