上标

We optimized reaction system for labelling-antibody in which the optimal amount of the purified anti-Stx2B IgG conjugated with colloidal gold beads was 60μg/mL, the purified antibody for E.coli O157 was 57μg/mL, the optimal pH was 8.2, the size of colloidal gold partical was 20nm, the optimization of stabilizing agent was BSA, the optimization buffer was boracic acid buffer with pH 8.2, the optimization of preserving fluid and eluant was boracic acid buffer with pH 8.2 including 5mM NaCl-1%BSA, confining liquid for NC membrane was 0.01% PBST with 3%BSA, the amount of polyclonal antibody against E.coli O157 and Stx2 conjugated with colloidal gold beads for conjugate pad was 3μg respectively, the amount of anti-Stx monoclonal antibody for test line was 0.1μg and 1μg for E.coli O157, the amount of goat anti rabbit IgG for control line of both GICA were 1μg.

测定了胶体金颗粒的最优化反应体系：胶体金颗粒的大小为20nm；抗体与胶体金溶液结合的最佳pH约为8.2；最佳蛋白结合量分别为抗大肠杆菌O157 IgG为57μg/mL，抗重组Stx2B IgG为60μg/mL；最佳稳定剂为BSA；最佳缓冲液为pH8.2硼酸溶液；最佳金标保存液和洗涤液为5mM NaCl-1%BSA的pH 8.2的硼酸缓冲液；NC膜的封闭液为3%BSA的0.01mol/L PBST；Stx2试纸条和大肠杆菌O157试纸条的质控线上的羊抗兔IgG的多克隆抗体最佳点样量均为1μg，其检测线的抗Stx2的单克隆抗体的点样量为0.1μg、抗大肠杆菌O157的单克隆抗体的点样量为1μg，结合垫上的金标抗大肠杆菌O157和重组Stx2B多克隆抗体点样量均为3μg。

A calibration model was established by introducing a planar target that could move freely with three collinear and known mutual location points. The calibration point coordinates in a camera coordinate system could be calculated by using the imaging information of three points and the light stripes on image plane of the camera.

引入一个可自由移动的平面靶标，靶标上只需要共线且相互位置确定的3个特征点，利用共线三点建立三点透视数学模型，根据3个特征点以及光条纹在摄像机像面的成像信息，获取了光平面上标定点在摄像机坐标系下的坐标。

The strap of a horse's harness that connects the girth to the noseband and is designed to prevent the horse from throwing back its head.

马颔缰一种马具，连接马的肚带和鼻羁的绳带，用以防止马向后甩头每一条线都联结一个标上3和一个标上4的顶点。这根管子连接下水道。

When you only need to detach from the backing sheet, just one click, you can stick to a variety of substrate surface, or use the labeller an automatic labeling on the production line.

应用时只需从底纸上剥离，轻轻一按，即可贴到各种基材的表面，也可使用贴标机在生产线上自动贴标。

When applied to the sample pad, the liquid sample migrates by capillary diffusion through the conjugate pad, rehydrating the gold conjugate and allowing the interaction of the sample analyte with the conjugate.

当液体样品用在样品垫上，液体由毛细作用力扩散通过金标垫，使金标复水并使得样品中的分析物特异性作用于金标。

AL500 Wrap Around Labeling Machine is suitable for many variety of bottle labeling. When labeling, the bottle will automatically rotate to allow the labeling device to apply the pressure for labeling.

详细信息： AL500全自动圆瓶贴标机适用于各类瓶子的贴标，贴标时，瓶子会自动旋转以让标签机将标签平均的黏贴在瓶子上。

KOAs that have clinical symptoms were "primary symptoms even and second symptoms acute", and in treatment the primary symptoms and second symptoms shoud be relieved at the same time, the first important is to relieve the second symptoms, then take an eye on primary symptoms.

导师许书亮教授认为膝OA为&本虚标实&、&本痿标痹&之证，有症状的膝OA 仍&标急本缓&，治疗上应标本兼顾，以治标为主但始终兼顾其本。

4 The above signs if it has also marked the valve plate can be re-marked.

5.4上述各项标志若已标在阀体上也可以重复标在标牌上。

4 The above signs if it has also marked the valve plate can be re-marked.

5.4上述各项标志若已经标在阀体上也能够反复标在标牌上。

Both of angular velocity of shoulder angle S(subscript S' and S(subscript F') or elbow angle E(subscript S' and E(subscript F') in frontal and sagittal view was having similar change pattern in second pull phase of snatch.

发力期肩关节矢状面角速度S(下标 S'与肩关节额状面角速度S(下标 F'有著相同的变化趋势，肘关节矢状面与额状面角速度E(下标 S'， E在变化上都有著相同的变化趋势。

In the negative and interrogative forms, of course, this is identical to the non-emphatic forms.

。但是，在否定句或疑问句里，这种带有"do"的方法表达的效果却没有什么强调的意思。

Go down on one's knees;kneel down

屈膝跪下。。。下跪祈祷