上切

Since Watson and Crick have brought forward the model of DNA helix structure and explain about it's reproduce mechanism in molecule level, research work concerning DNA has been continuously lucubrated. However, whether translating inheritance information and rebuilding DNA molecule via menstruating DNA sequence, or to date the most compelling gene project, chromosome protract et. al, there all need a sort of molecule scissors cleaving DNA, DNA cleavage reagent. At present, tool enzyme of cleaving DNA is mostly DNA restriction enzymes founded in 70 years, which only cleave DNA at specific 4-8 base pair sequences that are usually palindromic, so this restricts the at very fast speed developing of gene technology at certain extent.

自从Watson和Crick提出DNA的双螺旋结构并对其复制机制在分子水平上进行解释后，使得对DNA的研究不断深入，但无论是通过测定DNA的序列来破译遗传信息、改造DNA分子，还是目前最令人关注的基因工程、染色体绘制等，都需要一种断裂DNA分子的&剪刀&——DNA断裂试剂，目前断裂DNA的工具酶主要是上个世纪70年代发现的DNA限制性内切酶，但所知的Ⅱ型限制性内切酶的DNA序列识别长度较短（约为4～8个碱基对），而且一般要求回文结构，这就给飞速发展的基因工程技术带来一定的限制。

And theorically, the modified peaks are narrower and more symmetrical compared with the original peaks. Then the overlapped peaks were processed by middle-cutting method, and the deviation can be reduced.

这样从理论上说，经过处理的色谱峰与原始峰相比，会变得更窄更对称，这时再对重叠峰进行中切法处理，就可以在很大程度上减少简单中切法所造成的定量误差。

To raise welts or a welt on.A slash, as in a leaf or petal.

切痕深的切痕，如在树叶上或花瓣上的

Swing Beam Shear Cut the last knife in the process of arc around a fixed axis to swing through leverage, the fulcrum force is small, can increase cutting-edge life, the machine life, the whole structure is compact, and can non-polar adjust the amount of the trip on the tool carrier, greatly improving efficiency.

摆式剪板机的上刀架在剪切过程中绕一固定轴线作圆弧摆动，通过杠杆作用，支点受力小，可提高剪切刃寿命、机器寿命，整机结构紧凑，并能无极调节上刀架的行程量，大大提高工作效率。

The PCR products were examined by agarose gel electrophoresis. The target gene fragments were purified by gel extraction kit and ligated to cloning vector pMD18-T. The recombinant vectors were transformed into host strain E. coli K802 by lithium chloride method, screened and identified with PCR and restrictive enzymatic digestion. Their sequences were confirmed by DNA sequencing.(2) sTWEAK1 gene was subcloned into expression vector pProEx HTb and transformed into E. coli BL21. sTWEAK2 gene was subcloned into expression vector pMAL-C2x and transformed into E. coli TB1. The recombinant vectors were screened and identified with PCR and restrictive enzymatic digestion. The recombinant fusion proteins were induced to express with IPTG, detected by coomassie brilliant blue-stained SDS-polyacrylamide gel electrophoresis , and confirmed by Western blot analysis.(3) The sTWEAK1 fusion protein was purified with Ni-NTA Spin Kit.(4) The biological activity was assayed on transformed and tumor cells by microplate photometer after crystal violet or sulfur rodamine B staining.(5) The contents of IL-8 in the supernatant of 1990 cell cultures were determined by ELISA.(6) The morphological changes of the sensitive cells were observed by light and transmission electron microscopies.(7) The cell cycle and apoptotic rate were assayed by flow cytometry in 1990 and M85 cells.(8) The effect of fusion proteins on induction of NF-κB in 1990 and LOVO cells was detected with Dual-Luciferase Reporter Assay system.(9) The TWEAK gene was subcloned into Adeno-X Viral DNA with pShuttle vector and transfected into HEK293 cells by lipofectamine method.

(1)本研究用RT-PCR方法，从人组织细胞总RNA中扩增可溶性TWEAK胞外区（sTWEAK1和sTWEAK2）的cDNA序列及全长编码序列，用琼脂糖凝胶电泳分析PCR产物，胶回收目的基因片段，连接到pMD18-T克隆载体中，转化大肠杆菌K802，PCR和酶切筛选阳性克隆，全自动DNA测序验证序列；(2)sTWEAK1和sTWEAK2分别亚克隆到pProEx HTb和pMAL-C2x表达载体中，分别转化大肠杆菌BL21和TB1，PCR筛选和酶切鉴定，阳性克隆用IPTG诱导表达，表达产物用SDS-PAGE分析和Western blot验证融合蛋白；(3)用NTA-Ni Spin试剂盒初步分离纯化sTWEAK1融合蛋白；(4)用体外培养的肿瘤细胞和正常对表达产物进行活性检测，贴壁细胞用结晶紫染色法，悬浮细胞用磺酰罗丹明B染色法，酶标仪检测OD值；(5)敏感细胞用ELISA法检测细胞培养上清中IL-8的含量；(6)用光镜和电镜观察敏感细胞死亡和细胞凋亡情况；(7)用流式细胞仪分析表达产物对敏感细胞凋亡率和细胞周期的影响；(8)用双荧光素酶报告基因检测法，测定表达产物对敏感细胞NF-κB的影响；(9)用pShuttle穿梭质粒将TWEAK重组到腺病毒载体上，用脂质体转染法转染HEK293细胞，PCR鉴定重组质粒。

The dish washer includes one washer body with inside washing tank, rotary door in the front of the washer body, and one sealing latch set between the washer body and the door to raise the sealing property of the door. The sealing latch includes one hook unit set inside the hole on the washer body side, one sealing unit exposed outside the washer body and contacting closely to one side of the door in close state, one central slot for telescoping the hook unit, and one expansion joint in the joint part to avoid concentration of stress.

本发明包括洗碗机内部具备了洗涤槽的本体，设置在本体的前面转动的门，设置在本体前面和门之间，当关闭门时提高门和本体前面的封闭性的碰销；其中碰销包括：在本体前面一侧形成的插入孔中设置的挂钩部，露出于本体外部，在门关闭时，紧贴门一侧面的密封部，为了使挂钩在插入孔内直径方向伸缩，而在其中央部位形成的切缝，其特征在于密封部上形成了与切缝相通，防止挂钩伸缩时应力集中到挂钩部和密封部之间结合部位上的伸缩槽。

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我们的产品应有一个受欢迎的销售在国内市场并出口到32多个国家和地区的所有了为XXXXX椅子主要用于婚礼，宴会厅，酒店，教堂，为XXXXX主要产品有chivari椅子上，基亚瓦里椅子，宴会家具，城堡椅，折叠椅，总统椅，切尔滕纳姆椅子上，折叠宴会桌，卡米洛特Banqeuting主席，竹子联合椅子上，椅子上的城堡，保护高兴椅子上，椅子上舞厅， chivary椅子上， chiavary椅子上，椅子上的婚礼，租赁椅子，基亚瓦里椅子上，宿舍椅子上， chavari椅子上，教堂家具

Our products deserve a popular sales in domestic market and exported to more than 32 foreign countries and regions all over the xxxxx chairs main used in the wedding party, ballroom,hotel,church, xxxxx main products are chivari chair,chiavari chair,banquet furniture,chateau chair,folding chair,president chair,cheltenham chair,banquet folding tables,Camelot Banqeuting Chair,bamboo joint chair, castle chair, protect happy chair,ballroom chair,chivary chair,chiavary chair,wedding chair,rental chair,outdoor chair,chiavari chair,outdoor furniture,hostel chair,chavari chair,church furniture

我们的产品应有一个受欢迎的销售在国内市场并出口到32多个国家和地区的所有了为XXXXX椅子主要用于婚礼，宴会厅，酒店，教堂，为XXXXX主要产品有chivari椅子上，基亚瓦里椅子，宴会家具，城堡椅，折叠椅，总统椅，切尔滕纳姆椅子上，折叠宴会桌，卡米洛特Banqeuting主席，竹子联合椅子上，椅子上的城堡，保护高兴椅子上，椅子上舞厅， chivary椅子上， chiavary椅子上，椅子上的婚礼，租赁椅子，户外椅，基亚瓦里椅子，户外家具，宿舍椅子上， chavari椅子上，教堂家具

Our products deserve a popular sales in domestic market and exported to more than 32 foreign countries and regions all over the ***** chairs main used in the wedding party, ballroom, hotel, church,***** main products are chivari chair, chiavari chair, banquet furniture, chateau chair, folding chair, president chair, cheltenham chair, banquet folding tables, Camelot Banqeuting Chair, bamboo joint chair, castle chair, protect happy chair, ballroom chair, chivary chair, chiavary chair, wedding chair, rental chair, chiavari chair, hostel chair, chavari chair, church furniture

我们的产品应有一个受欢迎的销售在国内市场并出口到32多个国家和地区的所有了*****椅子主要用于婚礼，宴会厅，酒店，教堂，*****主要产品有chivari椅子上，基亚瓦里椅子，宴会家具，城堡椅，折叠椅，总统椅，切尔滕纳姆椅子上，折叠宴会桌，卡米洛特Banqeuting主席，竹子联合椅子上，椅子上的城堡，保护高兴椅子上，椅子上舞厅， chivary椅子上， chiavary椅子上，椅子上的婚礼，租赁椅子，基亚瓦里椅子上，宿舍椅子上， chavari椅子上，教堂家具

The sectional surface of the cut products are quite plane and smooth, it can save raw material.

切下的制品断面平滑，节约制品原料，对制品的切制可在生产线上随时同步进行，也可不在生产线上随时随样切割，切割平稳、无噪音。

Finally, according to market conditions and market products this article paper analyzes the trends in the development of camera technology, and designs a color night vision camera.

最后根据市场情况和市面上产品的情况分析了摄像机技术的发展趋势，并设计了一款彩色夜视摄像机。

Only person height weeds and the fierce looks stone idles were there.

只有半人深的荒草和龇牙咧嘴的神像。