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Moreover, the actual allele frequency of most varieties deviates far from Hardy-Weinberg equilibrium. All PPB、na 、I、h、Gi and Fst have proved to be the references to elucidate that ISSR is a most powerful tool to analyze genetic diversity, compared with the RAPD marker and the allozyme marker is less strong ordinally. We could divided the 70 samples into A, B, C, D and E five groups using three methods according to genetic distance clustering. There is a bit displacement for few varieties in different clustering maps, but the most are similar to morphological analysis despite that there is still a great difference among cultivars in the same one group. The above results imply that the three methods have the different sensitivity and resolution in genetic distance analysis of close varieties. The Mantel test indicates that the results from the three kinds of markers have the significant correlation, which demonstrates that the number of the used three kinds of markers is enough to exactly detect the diversity of all 70 samples to ideal extent. And these methods can be used to evaluate the diversity of the whole group using the miscellaneous samples instead of the individual sample, of the Gerbera jamesonii are mainly from tissue culture plants. In conclusion, the above study results provide a reference for the application of three kinds of molecular markers to molecular marker-assisted breeding of flower. 2. The genetic diversity among the eight introduced cut-flower varieties of Ranunculus asiatica was analyzed by the ISSR markers. Based on the genetic clustering tree, all the colorful flower varieties are clustered into one group, and the white flower varieties into another group. Moreover, among the former group the yellow flower varieties are clustered into one sub-group, and the reddish flower varieties, such as rose color, pink, nacarat, are clusetered into another sub-group.

由三种分子标记的分析结果可以看出,等位基因平均值、观察杂合度、Fis值、Fit值皆较高,表明非洲菊等位基因较丰富,杂合基因偏多,且绝大部份品种的实际等位基因频率在品种内偏离了Hardy-Weinberg equilibrium;PP8、na、Ⅰ、h、Gi及Fst皆表明,ISSR检测遗传多样性的能力最强,其次是RAPD,等位酶最低;根据遗传距离进行聚类,三种方法都把70个品种分成A、B、C、D、E五个大组,每一组中除少数品种发生位移外,大部份品种分类结果相似,且与形态分析结果有相似性,但在每一组中,品种间的聚类差别较大,表明这三种方法在近距离品种间检测遗传变异时灵敏度及分辨力不同;Mantel检测表明,三种标记的分析结果有显著相关性,表明所用的三种分子方法的标记数量已经可以相对无偏地检测到70个品种间遗传变异;非洲菊为组培苗,三种标记的检测结果皆表明,混合样品可以作为个体样品的代表,对整个居群的遗传多样性进行评价;这些研究结果可为三种分子标记方法在花卉分子辅助育种中的进一步应用提供借鉴。

The cytoarchitectonic organization ofprojections to the insular cortex and the amygdala from the parvicellular ventroposterior nucleus of the thalamus andtheparabrachial nucleus in the rat was investigated by means of horseradish peroxidase -tracing technigue; The relationship of origins of afferents to the insular cortex and the amygdala from the PBN was studied by using the retrograde transport of two fluorescent tracers; Thesynaptic connections of axonal terminals with the neuronsof the PBN were observed by means of electron microscopy.

GI主要位于岛皮质的尾侧部,DI和AI主要位于首侧部;VPpc是位于丘脑腹后核和内侧丘系之间的带状区,所含细胞较小(平均14u),其内侧部称为腹后内侧核小细胞部,其外侧部称为腹后外侧核小细胞部;PBN位于小脑上脚周围,由三部分组成:外侧亚核、内侧亚核和Kollikor-Fuse核,其中,PBl被分为7个区、PBm被分为3-4个区;杏仁核主要由三个细胞群组成:皮质内侧核群、基底外侧核群和中央核,中央核又有内侧部、外侧部和外囊部之分。

The stereo image pair is acquired by a pair of calibrated cameras. Global disparity of foot is estimated at first. After the image pair is preprocessed automatically, using non-contact information-collecting technique and phase-correlation method, which is based on complex wavelet, surface matching with sub-pixel accuracy in the frequency domain is carried out. By taking order matching constraint, continuity constraint and correlation constraint together, 3D point cloud of foot is reconstructed and 3D model of foot is built by mosaicking point cloud at various angles automatically. After normalization, high-order moments are compared, which is useful for the subsequent shoe last design.

运用已标定的双目照相机拍摄立体图像对,进行足部整体视差估计;在对立体图像对进行自动预处理后,采用非接触式的信息采集技术,运用基于复小波的相位相关技术对足部表面进行亚像素级小区域频域匹配,考虑顺序匹配约束、连续性约束和相关性约束条件,重建足部密集三维点云信息,并自动拼接不同角度点云生成相应的足部三维模型;对其归一化后,提取其高阶矩向量进行比对,为后续的三维鞋楦设计提供必要信息。

Her life and career were re-evaluated in the years around the centennial of her birth, in three major literary works and film retrospectives.

直到黄柳霜诞辰一百周年之际,在三部主流文学作品及她的电影回顾展中,她的生平和演艺事业得到重新评价。

As the centennial of Wong's birth approached, a re-examination of her life and career took shape; three major works on the actress appeared, and comprehensive retrospectives of her films were held at both the Museum of Modern Art and the American Museum of the Moving Image in New York City.

在黄柳霜的百年诞辰来临之际,人们审视起她的生活和事业重新;关于黄柳霜有三部重要作品,在纽约的现代美术博物馆和美国电影博物馆里对她的电影事业做出了综合的的回顾。

Early Acceptance: Katie was a big fan of the first three Potter flicks, so when she got to the set, she was "really starstruck and intimidated" by the rest of the cast.

相见恨晚:凯蒂是前三部HP电影的忠实影迷,所以当她来到片场时,她对其他影星&又迷又惶恐&。

A first: fat doctor first unique "control, activation, and reinforce" Liangxue UFA, three black for the world's a capture from white to black problems.

一个首创:发大夫首创独特&控制,激活,固本&凉血乌发,三部黑发为世界上的一个攻克白发转黑难题者。

Skim paragraph a and ask a student to describe toscanini in one sentence and the three world-famous operas conducted by him.

快速浏览语段,要求学生用一句话来描述toscanini,并在文中找出toscanini指挥的三部著名的歌剧。

In the third part, we design the performance management system on the base of the combination of theory and practical situation of SG company.

三部份是在结合SG公司实际情况的基础上,结合相关理论知识,为SG公司设计了绩效管理体系。

By analyzing the history of the South and McCullers's three important novels, this article mainly discusses the reasons for people's isolation and McCullers's contributions.

本文通过分析麦卡勒斯的三部具有代表意义的小说,并将其与美国南方的历史结合起来探讨了孤独的谜底以及麦卡勒斯的贡献。

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According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm~150rpm时取得最大值,此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世,形体几无变化。

When I was in school, the rabbi explained everythingin the Bible two different ways.

当我上学的时候,老师解释《圣经》用两种不同的方法。