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The results of its intrinsic fluorescence spectroscopy and fluorescence phase diagram showed that when the guanidine hydrochloride concentration in denaturation solution was about 1.0 mol/L, there existed a partially folded intermediate of Bacillus amyloliquefaciens a-amylase during its unfolding procedure, which followed a three-state model; the result of its fluorescence probe showed that when the guanidine hydrochloride concentration in denaturation solution was about 1.0 mol/L, there existed some stable hydrophobic regions, which could interact with a hydrophobic reagent 8-anilino-1-naphthalene sulfonic acid, in the partially folded intermediate of Bacillus amyloliquefaciens a-amylase; and the results of fluorescence quenching using acrylamide and potassium iodide as quenchers showed the distribution of Trp residues in Bacillus amyloliquefaciens a-amylase in different denaturation solution, with the maximum number (8) of tryptophan residues in a partially folded intermediate Bacillus amyloliquefaciens a-amylase molecule could be quenched by potassium iodide; and the results of their protein electrophoresis and SEC showed that no aggregate or aggregate precipitation of Bacillus amyloliquefaciens a-amylase formed during the whole unfolding procedure of Bacillus amyloliquefaciens a-amylase induced by guanidine hydrochloride.

内源荧光光谱和荧光相图结果表明,当变性液中盐酸胍浓度约为1.0 mol/L时,芽孢杆菌a-淀粉酶的去折叠过程中出现一个部分折叠中间体,其去折叠过程符合&三态模型&;荧光探针结果表明,在溶液中盐酸胍浓度约为1.0 mol/L时,中间态芽孢杆菌a-淀粉酶分子中存在着能够与探针分子1-苯胺基-8-萘磺酸结合的稳定的疏水区域;荧光猝灭研究给出了不同程度变性的淀粉液化芽孢杆菌a-淀粉酶中的Trp的分布情况,结果表明中间态芽孢杆菌a-淀粉酶分子中能够被碘化钾猝灭的位于分子表面的色氨酸残基数目达到最大的8个;蛋白电泳和体积排阻色谱结果表明,在盐酸胍诱导的芽孢杆菌a-淀粉酶分子的整个去折叠过程中,不会以共价键或非共价键形式形成芽孢杆菌a-淀粉酶分子之间的集聚体或集聚体沉淀。

Aminophenyl ketones can be synthesized from different anilines and nitriles in the presence of boron trichloride, or from anilines by Friedel-Crafts reaction.

邻氨基芳香酮则可以由不同取代的苯胺与不同的腈在三氯化硼的作用下生成,或者通过傅克酰基化反应得到。

The nerve axon regeneration length was measured 2 weeks after operation. The effects of peripheral nerve regeneration were evaluated by neural electrophysiology, the recovery rate of triceps surae muscular tension and weight and histological assessment 16 weeks after operation. Results All the animals survived till the end of experiment.

术后行大体观察;术后2 周测量神经轴突生长距离;术后16 周行电生理检测,计算术侧坐骨神经运动传导速度恢复率、术侧小腿三头肌收缩力恢复率及湿重恢复率,并行移植神经吻合中段HE 和半薄切片甲苯胺蓝染色观察。

The copolymers were systematically characterized by elementary analysis, FT-IR, UV-visible spectroscopy, solution high-resolution 〓H-NMR and solid-state high-resolution 〓C-NMR spectroscopes, solubility examination; circular dichroism technique was firstly used to characterize chain structure of the copolymers.

本文通过在刚性的PD聚合物分子链中引入邻乙氧基苯胺链节,使用化学氧化聚合和电化学氧化聚合两类新型聚合方法,合成了三种PD异构体与PHT的共聚物,并进行了聚合反应、结构和性能表征的系统研究,并且比较了氨基的相对取代位置对聚合活性的影响。

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I can not make it blossom and suits me

我不能让树为我开花

When temperatures are above approximately 80 °C discolouration of the raceways or rolling elements is a frequent feature.

当温度高于 80 °C 左右时,滚道或滚动元件褪色是很常见的特征。

The lawyer's case blew up because he had no proof.

律师的辩护失败,因为他没有证据。