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In this research, swainsonine contents of three Oxytropis species were determined by the high performance liquid chromatography coupled to mass spectrometry. The population genetic diversity was also studied and the swainsonine related molecular markers were screened by ISSR. In vitro fungi cultures from the individual plant of O. glabra were performed and microbiological morphology of the colony and the conidiophore were investigated. The 5.8S rDNA/ITS sequences of fungi amplified from total DNA in both plants and fungal endophytes were studied. These sequences were analysed and compared, and they were also compared to the 5.8S rDNA/ITS sequence of fungal enduphyte Embellisia sp. L12 from the O.lambertii, O.sericea and A.mollisimus distributed in USA.

本研究采用高效液相色谱-质谱联用法测定了三种棘豆植物的苦马豆素含量;通过ISSR分子标记技术对三种棘豆种群遗传多样性及其苦马豆素相关标记进行了研究;将小花棘豆植物单株进行体外真菌培养,研究了菌落和分生孢子的微生物形态学特性,从植物总DNA和相同植株培养的真菌总DNA扩增真菌的5.8SrDNA/ITS区,进行了DNA序列分析与排序;联系单株小花棘豆的苦马豆素含量测定和内生真菌ITS序列分析结果,与从美国分布的二种棘豆(O.lambertii、O.sericea)和一种黄芪(A.mollisimus)中分离的内生真菌埃里砖格孢属Embellisia sp.L12株得到的结果进行了比较。

In this dissertation we propose a set of fast algorithms for computing the DGFT (Discrete Generalized Fourier Transforms) over the hexagon domains and the parallel dodecahedron domains and give the parallel implementation on Linux clusters.

本文在理论上的主要贡献就是利用多色排序、区域分解和多重网格的思想,设计了二维三方向平行六边形区域和三维四方向平行十二面体区域上广义离散傅立叶变换的快速算法并给出它在PC Linux机群上的并行实现。

This study firstly based on Kubelka-Munk law, and adopted the method of three stimulate value match, then established mathematical model of hectograph special color printing ink that was about tricolor ink. Moreover it presented calculation method of parameter that was necessary to answer model.

本论文首先以库贝卡--芒克定律为基础,采用三刺激值相匹配的方法,建立了以三原色油墨为基本配色墨的胶版印刷专色油墨的配色数学模型,并给出了配色模型所需参数的求解方法。

As for Ukiyo-e prints, Suzuki Harunobu reached his prime time at his late years. For example 《Romantic Voluptuous Little Pretty》,《the Eight Scenes of Beauty's Chamber》《Romantic Beauty Mirrors》, this group of three of beauties are very famous. Kitagawa Utamaro had unique aesthetic appreciation, presenting women's charming and grace. Another artist Toshusai Sharaku didn't created many works. He appeared only for a short period of time, just like a shooting start crossing the sky. Katsushika Hokusai's "the Thirty-six Scenes of Fuyue" was the beginning of the popularity of Ukiyo-e prints. His work absorbed the factors of western world. Many of his works presented grandeur in front of people. The works of Utagawa Hiroshiga were also outstanding. His works revealed a sort of " Japanese complex".

浮世绘方面,铃木春信的创作旺盛期是在晚年,《风流艳色小豆人》、《风流闺室八景》和《荡女鉴镜图》三组美人画非常著名;喜多川歌磨有着独到的审美意识,展示女性的妩媚动人和优雅神态;役者绘大师东洲斋写乐的作品不多,仅仅出现在一个极短的期间,犹如历史夜空中一闪而过的彗星;葛饰北斋的"富岳三十六景"是浮世绘风景画流行的开端,作品吸取西洋的绘画元素,大多雄伟壮丽;歌川广重的"名所绘"出类拔萃,画中流露一种"日本情结"。

In every group, rabbits were subdivided into experimental and control subgroups. 2 Rabbits were bullets injected followed by continuous injected with 13C labeled leucine, glucose, and lactic acid; 3 Blood were drewed before and 150, 160, 170, and 180 min after the initiation of isotopes injection for material analysis; 4 Exhale gas were collected every 5 min in the first 30 min followed by every 30 min there after for material analysis; 5 After centrifuge in low temp the supernatant of the blood samples were collected and went through axon and anon exchange column treatment; 6 Treated blood sample was used for 13C labeled leucine, glucose lactic acid examination through mass spectrograph; 7 The exhale gas was collected for 13CO2 exam through gas-phase mass spectrograph; 8 CO2 total production rate (V13CO2), body various substances production, oxidation speed, and substances metabolic percentage all can be calculated through equations provided by references below; 9 Data was processed through student t test.

分亮氨酸、葡萄糖和乳酸三组各取健康新西兰兔16只,每组再分为高代谢脓毒症组和对照组两部分,建模方法同第一部分;2)三组分别先静脉弹丸式推注再持续灌注13C标记的亮氨酸、葡萄糖和乳酸;3)灌注前抽取动脉血作为背景值,灌注达150,160,170和180min分别抽取动脉血2ml用于质谱分析;4)实验开始后前30min中每5min收集呼出气一次,随后每30min收集呼出气一次,用于气相质谱分析;5)血样经过低温离心取上清液,过阳离子及阴离子交换树脂,再经衍生化处理;6)处理过的血样进入气相色谱-质谱仪,测量其13C标记的亮氨酸、葡萄糖和乳酸丰度;7)实验兔的呼出气通过13CO2气相质谱分析仪测定其中的13CO2丰度(E13CO2);8)利用文献提供的公式算出CO2总生成率(V13CO2)以及机体各物质的通量、氧化速率以及物质代谢百分比等;9)数据分析处理采用t检验。

Colour Tetris changes Tetris in that when three or more tiles of the same color in a multicoloured blocks touch in any direction they eliminate themselves.

颜色为Tetris Tetris的变化在时,三个或三个以上的瓷砖相同的颜色在一个多色座触摸在任何方向,消除他们自己。

The transformation between I3F,C3F,t3F and YF of three generation quarks and leptons of Fermi type follows the hypercharge-spinor transformation,and so does that between I′3B,C′3B,t′3B and Y′B of their three generation supersymmetry companion s,antiquarks and antileptons of Bose type.

三代费米型夸克、轻子的I3F,C3F,t3F,YF间及它们的超对称伴子三代玻色型反夸克、反轻子的I′3B,C′3B,t′3B,Y′B间均满足超荷-旋量变换。

Using self-made conventional PPOP as reference, relative bioavailability and pharmacokinetic study of novel PPOP and novel SOTs were performed in six beagle dogs by three-circle crossover design, drug concentration in plasma of beagle dogs after single dose oral administered of three kinds of osmotic pumps was detected by HPLC method.

本文采用高效液相色谱法,以自制的传统型格列齐特推拉式渗透泵片为参比制剂,以自制的双面打孔格列齐特推拉式渗透泵片和异步释放格列齐特三层渗透泵片为受试制剂,对三种格列齐特渗透泵制剂进行了beagle犬体内药物动力学研究。

We collected Leishmania promastigotes,washed its with 0.01mol/LpH7.2 buffers ,smashed its,and then measured protein content and titrated work concentration.2.We tested Kala-azar patients"serum and healthy persons"serum of epidemic area by ELISA. The results of three antigensmethods were the same, work concentration of serum was the same(l :100).Results:The protein content of three antigens is, 901 strain with 1000ug/ml,801 strain with 960ug/ml and 951 strain with 1100ug/ml. Suitable work concentration is, 901 strain with 10ug/ml ,801 strain with 12ug/ml and 951 strain with 11ug/ml.

1、抗原的提取:收集新疆不同地区分离得到的利什曼原虫前鞭毛体,经培养,用0.01mol/L pH7.2的磷酸盐缓冲液漂洗,超声粉碎,比色法测定其蛋白质含量,间接ELISA法滴定出抗原的工作稀释度。2、检测:用提取的三株抗原,分别进行间接ELISA法测定黑热病病人血清和疫区健康人血清抗体,并以肺结核病人血清、非疫区健康人血清做对照,三株抗原所用检测方法一致,血清工作稀释度均为1∶100。

My clothes used to arrive twice a year, in white boxes from London; they were sensible and they never varied. In summer I wore sea island cotton undershirts and, in winter, woolen ones with sleeves. I wore long socks or woolen stockings in winter, and short cotton socks in summer. I had three kinds of shoes: stout brown lace-ups, stout brown sandals, and flat pumps, made of bronze kid, which were reserved for parties, although I went to very few parties. In summer I wore cotton frocks and cardigans Jenna Knitted; in winter I wore gray flannel pleated skirts and gray flannel jackets. I had a succession of Harris tweed overcoats with velveteen collars, all of which were identical; a succession of identical pudding basin hats that clipped under the chin with elastic. I hated the scratchy winter vests, but apart from that, I never thought about my clothes a great deal, except when I went to visit my great-aunt Maud in London.

俺滴衣服每年两次,用白色盒子的盒子装着,从伦敦送达;我可以预见他们是怎么样的,他们从来没有改变过夏天,我穿海岛棉汗衫,冬天则是有袖的羊毛衫我在冬季穿长袜子或羊毛袜,夏天穿短棉袜我有三种鞋:褐色系带短帮鞋,啡色凉鞋和派对上穿的啡色小山羊皮平底舞鞋,虽然我很少参加派对夏天,我穿着棉上衣和珍娜织的开衫;冬天,我穿灰色法兰绒打褶短裙和灰色法兰绒外套我有系列的Harris斜纹软呢大衣,他们的衣领都是仿天鹅绒的,所有的这些都是完成一样的;一致的布丁一样圆圆胖胖,下巴位置下面有弹性的帽子我讨厌那种令人觉得发痒的冬季背心,但除此之外,我从来没有想过我的衣服着装是一件多么了不起的大事,除了当我去拜访在伦敦的莫德姑婆

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However, as the name(read-only memory)implies, CD disks cannot be written onorchanged in any way.

然而,正如其名字所指出的那样,CD盘不能写,也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口,替代木托盘,免薰蒸,符合欧盟、北美各国对出口货物包装材料的法令要求;喷涂钢托盘适用于重载上货架之用,托盘表面根据需要制作成平板状、波纹状及间隔形式,满足不同的使用要求。

A single payment file can be uploaded from an ERP system to effect all pan-China RMB payments and overseas payments in all currencies.

付款指令文件可从您的 ERP 系统上传到我们的电子银行系统来只是国内及对海外各种币种付款。