三维

Homology modeling of 3-D structure of two AChE from L.entomophila were constructed using H.sapiens(1p0i:A) native BuChE structure and Drosophila melanogaster(1d×4:A) native AChE structure as templates,respectively,by SWISS-MODEL.The catalytic triad were found and denoted in the 3-D structure of AChE from L. entomophila referring to T.californica.2.2 Gene cloning ofβ-actin and mRNA expression levels of two AChE genes from L. entomophilaBecause no reference gene has ever been used in Real Time PCR for L.entomophila in GeneBank,a fragment ofβ-actin gene was cloned from L.entomophila(GenBank Accession No.: FJ041117).It consists of 822 bp encoding a protein of 273 amino acids residues.

利用蛋白质结构同源建模工具，分别以人丁酰胆碱酯酶(1p0i：A)和果蝇乙酰胆碱酯酶(1d×4：A)的蛋白晶体结构为模板，对嗜虫书虱2个AChE的三维结构进行同源建模，并在三维结构中发现了AChE的酶解活性位点，证明嗜虫书虱体内也存在2个AChE基因。2.2嗜虫书虱β-actin基因克隆及乙酰胆碱酯酶基因mRNA表达水平研究目前关于嗜虫书虱的分子生物学研究较少，在GenBank中没有可用作内参基因的序列，因此本研究从嗜虫书虱体内克隆获得β—actin基因片段(GenBank登录号：FJ041117)，该片段长度为822 bp，编码273个氨基酸残基，同源性比对分析表明该片段与其它昆虫的β—actin基因具有很高的同源性。

Several steps including ct scan, target contouring and reconstruction of 3d digital structures were taken before treatment plans were designed through a cadplan computerized system which allowed a 3d crt and 4 imrt plans with 3, 5, 7 and 9 equispaced coplanar intensity modulated beams to be produced.

选取5例直肠癌病例，进行ct扫描、靶区勾画和三维图像结构重建，分别在cadplan治疗计划系统上进行三维适形和空间等分的3、5、7、9野的调强放疗计划设计，并对每一计划作出评价。

Methods A single shot 3-D laser scanner, VIVID 910, was used to capture the craniofacial images of 227 patients. Only a top light was used during scanning. The patients sit up straight with their Frankfurt plane parallel to the horizontal plane, and the shot was 10° upper-wards. The scans were applied frontally and 80°bi-laterally, and the data were registratered, merged and processed with a general reverse engineering software. The usage time and the image quality were documented.

利用VIVID 910单镜头三维激光扫描仪扫描227例受试者的颜面三维影像，采用全室内顶置光源，受试者眶耳平面尽量与地面水平，扫描仪镜头水平抬高10°，使用14mm焦距镜头，于受试者正前方及双侧各80°位置采集数据，获得数据经通用软件拼接、二期处理，统计扫描及数据处理所需的时间及扫描结果质量。

Drug sensitive test and three-dimensional test220 strains of Pa were isolated from hospitalized patients between 2003 and 2007. K-B method was used to tested the susceptibility of 10 different antibiotics. IRPa was screened by testing the minimal inhibitory concentration of imipemem by using agar diluiion method.The susceptibility of these IRPa to the antibiotics was analysised. Three-dimensional test was used to identify the different kinds of beta lactamases from 220 strains of Pa.2.Carbarpenems hydrolytic enzyme genes and oprD2 gene were detectedamong the selected IRPa strains, PCR method was performed to detect carbapenemase genes which included GES、KPC、SPM、VIM、IMP、GIM gene and the oprD2 gene;Multiplex PCR were used to detect OXA genes and plasmid-mediated AmpC beta lactamase genes; The expression of the chromosomal AmpC beta lactamases and oprD2 genes in IRPa strains were analyzed by Real-time PCR.3.Identification and characterization of integronsIntegrase gene was detected by PCR, and the classification of integrons was performed by using restriction fragment length polymorphism.PCR was performed to detect the qacE△1-sull gene,and the gene cassetes which are located at variable region of integrons in the strains were detected to be positive.

方法1、药敏实验和三维实验收集2003～2007年临床分离的220株Pa，对这些菌株采用K-B法测定10种临床常用抗生素的药敏情况，同时采用琼脂稀释法检测亚胺培南的最低抑菌浓度(Minimal inhibitory concentration，MIC)，筛选出对亚胺培南耐药的铜绿假单胞菌，并分析其对其它抗生素的药物敏感率；采用三维实验的方法分析220株Pa产β内酰胺酶的类型。2、碳青霉烯类水解酶和oprD2蛋白的检测针对鉴定的IRPa菌株，采用普通PCR方法检测具有碳青霉烯水解作用的β内酰胺酶耐药基因(GES、KPC、SPM、VIM、IMP、GIM基因)和oprD2基因，采用多重PCR的方法检测OXA型基因和质粒携带的AmpC酶基因，用荧光定量RT-PCR方法检测oprD2蛋白基因表达情况；同时对产AmpC酶的Pa(25株，含IMP耐药和敏感株)用RT-PCR方法检测AmpC酶基因的表达量情况。3。

Our study demonstrates that cardiac cells possess the ability to reestablish 3-D tissue architecture in vitro;Simulated microgravity by HARV bioreactor facilitates the metabolism and function of the 3-D cardiac cell cultures.

体外培养的乳鼠心肌细胞具有形成具有同步自律收缩的三维组织结构的能力；模拟微重力的培养环境有利于改善心肌细胞三维组织样培养物的代谢和功能。

In this paper,a three dimensional elastic plastic hydrodynamical numerical method and the code MEPH3D using this method which can be used on high speed impact problems are introduced.

介绍一种可用于高速碰撞问题数值模拟的三维弹塑性流体动力学数值计算方法，以及应用该方法研制的应用软件MEPH3D，并给出了一些高速碰撞问题的数值计算结果，计算结果表明该程序具有计算三维高速碰撞问题的能力。

This paper will discuss in the first part how the data of borehole distances being transmitted from an ichnographic layout to the section planes employing a computer program, and a method of merging analogy soil sub-layers to a major layer with the same statistical properties to facilitate the mechanical analysis using the fuzzy clustering is introduced.

讨论了用程序编制的方法实现的数据从平面图到剖面图的钻孔距离传递，利用模糊聚类思想提出的对剖面数据进行合并的建议和利用地质三维软件实现的三维地质体模型的耦合展示，这几个问题正是目前大多勘察单位在数据控制与传递流程中可能欠缺的环节。

In this paper, the 3-D finite element relocating mesh method is used to calculate the temperature field and the thermal creep stress field of spillway dam and mid level outlet dam in certain roller compacted concrete gravity dam, sensitivity analysis have been down with different parameters, such as placing temperature, the location of cooling pipe, cooling temperature, time for water supply, ascended-layer of concrete, intermissive time and different placing time, and the commendatory construction scheme have been put forward in such analysis; according by the test result on the two scheme of concrete aggregate combination (basalt as coarse aggregate and limestone as fine aggregate, basalt as coarse aggregate and basalt as fine aggregate), simulation analysis on temperature field and thermal creep stress field of the commendatory construction scheme have been down, and analysis have also been down on temperature field and thermal stress field of the two different aggregate combination, the result indicates that the spillway dam and mid level outlet dam which adopted these two aggregate combination can both satisfy the require of design crack resistance in concrete, furthermore limestone as fine is better than basalt as fine aggregate; the effect of summer water storage and winter water storage on the temperature field and thermal stress field have been analyzed, comparing with winter water storage, in summer water storage scheme the maximum tensile stress at abnormal concrete in upstream fill is decreased by 0.1～0.3MPa and there are no evidence change of the maximum tensile stress at other parts, which indicate that summer water storage is avail for the crack resistance of upstream fill; according to the simulation calculating result and comparing temperature simulation calculating results and temperature controlling measures of some similarity projects both completed and construction, synthesis appraise have been carried to the design temperature controlling measures of dam, and the temperature controlling measures of this project have been brought forward, all these have great reference value on the design of temperature controlling measures and the optimization of the construction scheme.

本文采用三维有限元浮动网格法仿真计算程序，对某碾压混凝土重力坝的溢流坝段和中孔坝段分别进行了三维有限元温度场和温度徐变应力仿真计算分析，对浇筑温度、冷却水管的布置方式、冷却水温、通水时间、浇筑升程和间歇时间、不同开始浇筑日期等参数进行了敏感性分析，在此基础上提出了推荐的施工方案；根据两种骨料方案(玄武岩粗骨料+灰岩细骨料方案；玄武岩粗骨料+玄武岩细骨料方案)的混凝土性能试验研究成果，对推荐的施工方案进行了温度场和温度徐变应力仿真计算，并对比分析了两种不同骨料情况下的温度场和温度应力，结果表明溢流坝段及中孔坝段采用上述两种骨料均能满足混凝土的抗裂设计要求，且灰岩细骨料优于玄武岩细骨料；计算分析了夏季蓄水和冬季蓄水对坝体温度场和温度应力的影响，夏季蓄水方案与冬季蓄水方案相比，坝体上游变态混凝土区域最大拉应力小0.1～0.3MPa，其他部位最大拉应力无明显变化，夏季蓄水对坝体上游面混凝土的抗裂有利；根据仿真计算成果并通过比较国内一些已建和在建的类似工程的温度仿真计算成果及采取的温控措施，对大坝的设计温控措施进行了综合评价，并提出了该工程的温控措施，对温控设计和优化施工方案具有重要参考价值。

Methods A wet hip joint's sample from corpse was scaned by CT and all images of every cross-section hip joint's layer was obtained, and an hip joint's model was contructed with Unigraphics NX 2.0 and Solid Works 2006 SP 0.0 software. The models were assembled when simulated total hip arthroplasty after total hip joint prosthetic and ischiofemoral ligament model had been constructured. Analysis was done on von Mises stress distribution and amount of total hip component when simulated seated leg-crossing manuver. The bone and metal components were meshed as rigid bodies, composed of three-dimensional, all-quadrilateral rigid body elements, and hip joint capsule ligament was fully three-dimensional, hexahedral continuum elements representations. Results The current form of this ischiofemoral ligament's reconstruction finite element model provided for multi-body contact, large displacement interfacial sliding, large deformation capsule ligament representation, and clearly reflected the real ischiofemoral ligansent's anatomy and biomechanical behavior. The maximum on Mises stresses at the joint interface was more than that fur an otherwise identical hardware-only construct.

选择成人尸体髋关节行CT成像得到髋关节每层横截面图像，提取边界坐标，利用有限元分析软件Unigraphics NX 2.0和Solid-Works 2006 SP0.0构建出髋关节三维有限元模型及人工髋关节假体，并模拟全髋置换术进行装配，将坐股韧带按其术中重建位置进行构建骨骼与金属模型采用三维十节点四面体实体单元进行网格划分，关节囊六面体连接单元划分，模拟坐位腿交叉动作载荷获取假体撞击过程的角活动度和相应的von Mises应力分布情况结果所构建的多体连接、大界面划移、大变形的坐股韧带重建有限元模型，客观反映坐股韧带真实解剖形态及生物力学行为，增加坐股韧带重建的模型其关节面的最大主应力值大于仅有金属的模型。

Directly reading the 210-layer of 0.65-mm-thick consecutive CT Dicom format images of human body thoracolumbar making use of 0.625-mm isotropous resolution thin layer scanning technique into the Mimics. Defining bone organization threshold, withdrawing each outline, partitioning each edge of layer picture, edited selectively and repaired by hole processing. After a redundancy data was cleaned, the thoracolumbar 3-D finite element geometry model was acquired. Then area mesh could be kept as the document Ansys named .lis as the suffix and be introduced into Ansys software directly to mesh body, and then transferred the body mesh to mimics to assignment son the basis of CT values, once again import Ansys to establish 3-D finite element model.

将以各向同性分辨率0.625 mm薄层扫描所得的层厚0.65 mm人体胸腰段连续断层210层Dicom格式CT图像，直接读入Mimics后界定骨组织阈值、提取各层面轮廓线、图像边缘分割、选择性编辑及补洞处理，去除冗余数据，三维化处理后获得胸腰段三维几何面网格模型，将其保存为后缀名。lis的Ansys文件，直接导入Ansys有限元分析软件进行体网格划分，再将体网格转入Mimics根据CT值给予赋值，再次导入Ansys生成有限元模型。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。