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Part three: An experiment study on the repair of peripheral nerve injury by NT-3 gene modified neural stem cells objective To investigate the effective treatment of peripheral nerve injury with NT-3 gene modified neural stem cells, to explore the feasibility of the treatment by means of gene transfection Methods 54 SD rats were divided evenly into 3 groups. The sciatic nerve transected model were made and sutured end to end with epineurium. The neural stem cell without NT-3 gene or with NT-3 gene modified were injected into triceps muscle one time a week, saline injected as control group. The nerve trunks of stomas and triceps muscle were acted as specimen three weeks、six weeks and nine weeks after operation.

最后,为了探讨神经营养素-3基因修饰的神经干细胞对周围神经再生修复的影响,从转基因角度探讨治疗周围神经损伤的有效方法,我们选用54只SD大鼠随机分为3组,造成坐骨神经切断损伤模型,神经外膜端端对位缝合后,于小腿三头肌每周分别注射生理盐水、未被神经营养素-3基因修饰的神经干细胞、及神经营养素-3基因修饰的神经干细胞。

Results The reconstructed results successfully reappeared three dimensional structure of fasciculus inside the sciatic nerve and showed the topographic anatomy of every nerve fascicle and its relationship in arbitrary sections, and presented patterns of complicated recombination of nerve bundles in the whole length.

结果 三维重建真实地再现坐骨神经内部各神经束的三维立体结构,并可显示坐骨神经中神经束的任意断面及其全长的解剖结构与相互关系,形象地展示坐骨神经内部神经束的复杂重组过程。

The notable proliferation was not observed by eyes in the local of injection. The infiltration of inflammation cells and mild proliferation of fibrocyte around dura mater was observed by HE stained in 4 and 8 weeks after injection. Infiltration and exudation of inflammation cells was observed by HE stained in epidural nerve root. Compared with group A, no changes of group B, C and D were observed under specific stained. Proliferation of type Ⅱ collagen fibers around dura mater was seen under immunohistochemical stained in 4 and 8 weeks after injection. There is no significant demyelination changes under LFB stained. The thickness and shape of the myelin sheath in epidural nerve root was not regular under transmission electronic microscopy in 4 and 8 weeks after injection. Fibroblast was also seen there. In nerve endometrium, macrophage could be seen under TEM, myelinated nerve fiber changed significantly, but nonmyelinated nerve fiber changed mildly. When 8 weeks, the changes of group D is smaller than the group B and C.

给药局部肉眼观察未见明显的纤维组织增生;HE染色可见B、C、D三组给药后四周及八周时硬膜内外均有炎细胞浸润,纤维细胞轻度增生,硬膜外神经根内有炎细胞浸润及炎性渗出;特殊染色B、C、D三组同A组相比未见有脊髓及神经根的改变;免疫组化染色,给药后四周及八周时,硬膜内外均有Ⅱ型胶原纤维增生;固兰染色B、C、D三组未见有明显脱髓鞘改变,与A组相比无明显异常改变;电镜观察B、C、D三组在给药后的四周及八周时,表现为硬膜外神经根内髓鞘厚薄不一,形状不规则,可见成纤维细胞,神经内膜中可见有巨噬细胞;粗大的有髓神经纤维变化明显,无髓神经纤维受累较轻;八周时电镜下D组改变较B、C两组为轻。

At first, with the low-frequency data measured, three of the LRE fault detection systems for the real-time condition are proposed using the nonlinear identification technology of the BP neural network, the state estimation technology of the dynamic neural network, and the pattern recognition technology of the fuzzy hypersphere neural network. The learning algorithms of the BP and dynamic neural network are researched at the same time. Furthermore, while the engine operation is divided into the start and steady-state processes, the real time ability, the response time, the accuracy, the robustness, the sensitivity and the monitoring parameter optimization are studied. In the test data analyses of the YF-75 engine, the detection system correctly carried out for all normal tests, and in the three abnormal tests the engine faults were accurately forecasted.

首先,基于低频测量数据,采用BP神经网络的非线性辨识技术、动态神经网络的状态估计技术及模糊超球神经网络的模式识别技术,提出了三种发动机故障实时检测系统;同时研究了BP神经网络和动态神经网络的学习算法;另外还把发动机工作分为启动过程和稳态过程,分别讨论了神经网络故障检测系统的实时性、及时性、准确性、鲁棒性、敏感性及参数优化问题;在YF-75发动机试车数据分析中,不仅全部正确地监测了正常试车过程,而且准确地预报了三次异常试车中的发动机故障。

The other cancers with shorter follow up had three years overall and event free survival as follow: 75% and 62% for Medulloblastoma, 74% and 67% for Non-Hodgkin Lymphoma, 100% and 91% for extracranial Germ Cell Tumour, 30% and 20% for advanced neuroblastoma, 68% and 52% for Ewing's Sarcoma, 87% and 79% for Wilms' Tumour, 82% and 80% for Osteosarcoma, 56% and 45% for Rhabdomyosarcoma, and 60% and 50% for Hepatoblastoma respectively.

其他癌症经三年的短期随访,总存活率及无并发症存活率分别是:成神经管细胞瘤为 75%及 62%;非何杰金氏淋巴瘤为 74%及 67%;颅外的胚细胞瘤为 100%及 91%;已播散成神经细胞瘤为 30%及 20%;尤因氏肉瘤为 68%及 52%;威尔姆斯氏瘤为 87%及 79%;骨肉瘤为 82%及80%;横纹肌肉瘤为 56%及 45%;肝母细胞瘤为 60%及 50%。

In three-valued neural networks model, the energy function of two-valued neural networks model can not be applied, so the energy function need to be find for the three -valued neural networks model, and here two kind model algorithms are compared in this paper.

在三值神经网络模型中,二值模型的能量函数不能适用于三值模型,因此需要求解三值模型的能量函数,在此本文对三值神经网络测试生成算法与二值神经网络测试生成算法进行比较。

High voltage stimulation was applied to elicit MEP and record latent period of MEP at all segments and the motor nerve conduction velocity of the infraclavicular segment.

采用尺神经近段MEP、锁骨下近段MNCV及全程MEP法检测,三方法的阳性率分别为94.4%、83.3%、55.6%。说明近段MEP检出百分率高于锁骨下近段MNCV的检出率,高于全程MEP潜伏期的检出率。

Specifically, Muscat's work -- peer reviewed and completed according to a specific protocol under the WTR -- identified a near tripling in the risk neuroepithelial tumors and a correlation between the side of the head where the phones were used and the side of the head where the tumor was located that were both statistically significant.

具体来说,马斯喀特的工作-同行审查并完成了根据具体的协议下WTR -确定了近三倍的风险和神经肿瘤之间有关联一侧的脑部出现的手机被用来和一侧的脑部出现肿瘤位于这都是统计学意义。

The system makes use of cooperation of artificial circuit and digital circuit to produce tristate neural stimulating signal and achieve signal collec-tion and LCD screen real time display of stimulating .

利用模拟电路和数字电路配合产生了三态的神经刺激信号。基于DSP控制成功的实现了信号采集和刺激的液晶屏实时显示。

The SG showed highest FXPRLamide peptides titer in neural organs. Using an antiserum against H. armigera PBAN, PBAN-like immunoreactivity was detected in the SG and TG of Antheraea yamamai by whole-mount immunocytochemistry, and there were three clusters cells in the SG which shows positive PBAN-like immunoreactivity. The titers of FXPRLamide peptides immunoreactivity in the hemolymph kept a steady level. When pupation, the titer increased promptly, but decreased to a low level after early pupal stage.

这证明了天蚕可能存在FXPRLamide家族神经肽中的DH-PBAN基因;天蚕的咽下神经节中FXPRLamide神经肽含量最高,咽下神经节有三簇细胞合成FXPRLamide神经肽,在胸神经节和腹神经节也有明显的阳性细胞;天蚕5龄幼虫的血淋巴中FXPRLamide神经肽含量比较稳定,化蛹前含量升高,蛹期含量降低。

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