三核的

Barley seeds were irradiated with five different doses of 60 Co-γ ray.There were many kinds of chromosome aberration in pollen mother cells,such as bridge,laggards in anaphase I and anaphase II,asymmetrical division in anaphase I and asynchronous division in anaphase II,ring quadrivalent in metaphase I,three pole division and micro-nuclei in telophase I,micro-nuclei in tetrad and some micro-nucleus separates from the tetrad to form polyad.

大麦种子经5种不同剂量γ射线辐照后， M1植株的花粉母细胞出现多种染色体畸变类型，例如后Ⅰ、后Ⅱ中出现桥、落后，后Ⅰ中出现不对称分裂，后Ⅱ中出现不同步分裂，中期Ⅰ出现环状四价体，末期Ⅰ中有三极分裂和微核，四分体中出现微核且有的微核独立于四分体，从而形成多分体。

In chapter III,we consider the wavelet method for the approximation of the nu-merical Bergman kernel.It is very clear that the Bergman kernel plays an importantrole in the complex analysis and elliptic equations.The Bergman kernel has a closedrelation with the Riemann mapping,Green function,Neumann kernel and the Szeg〓kernel etc.

第三章研究了数值Bergman核数值逼近的小波方法，Bergman核在复分析和椭圆型方程中占有重要地位，它与Riemann映射、Green函数、Neumann函数、核等有着密切联系。

The results proclaimed that nucleator C caused the thinner spherulites of POM and a greater growth rate, which was a three dimensional heterogenous growth process, and the crystal system of POM was not changed, but the crys-tallinity of POM decreased, the crystallite size of POM increased.

实验果表明，成核剂C的加入，使POM球晶细化，结晶速度加快，结晶过程为异相成核三维生长过程，且不改变POM的结晶晶型但结晶度略有下降，微晶尺pp成核剂寸增大。

When EMS was applied, it was found that 2.0% EMS resulted the highest microkernel rate (15.6‰). Treating the materials with 2.0% EMS for 4 hours could lead to semi-lethal. Cytological observations found that chromosome aberration phenomena such as single microkernel, double microkernel, three microkernel, chromosome bridge, resort chromosome and syncytium were presented in the mutants.

EMS诱变途径中，浓度为2.0 %处理时间为4 h的组合获得最高的微核率（15.6‰），EMS浓度为2.0 %处理时间为4 h的组合使材料达到半致死；细胞学的观察看到了单微核、双微核、三微核、染色体桥、滞留染色体和合胞体等染色体畸变现象，再生苗中发现了1棵变异株。60Co辐射的诱变途径中，20Gy的剂量使不定芽半致死，再生苗中发现了1棵变异株。

The invention concerns an artificial block comprising a central core (1) having first (11) and second (12) opposite surfaces each provided with an anvil-shaped side leg (21, 22) with a table (21A, 22A), two sloping sides (21E, 21G; 22E, 22G) and two side surfaces (21B, 21C; 22B, 22C), the tables of said side legs being arranged parallel to said first and second opposite surfaces of the core, the inclined sides of said side legs resting on third (13) and fourth (14) exposed opposite surfaces of said core, said core having fifth (15) and sixth (16) surfaces each provided with side legs and front legs shaped like a truncated pyramid with four side surfaces and one front surface (33) parallel to said fifth and sixth surfaces of the core and to the side surfaces of the side legs.

一种保护层块料，包括一个中心核体(1)，有相对的第一面(11)及第二面(12)，每一面上各有一个砧状侧凸起(21，22)，这两个侧凸起各有一个台面(21A；22A)、两个斜面(21E，21G；22E，22G)和两个侧面(21B，21C；22B，22C)，所述台面相互平行，而且平行于所述中心核体的相对的第一面及第二面，所述侧凸起的斜面支撑于所述核体的相对的可见的第三(13)和第四面(14)。所述核体还有相对的第五(15)和第六面(16)，其上各有一个锥台状的前凸起，该前凸起有四个侧面和一个前表面(33)，该前表面平行于所述核体的第五和第六面，还平行于侧凸起的侧面。

We are satisfied that thefive main components were pure and plenty enough for NMR study. The structure and configuration of key component of urushiol weresuccessfully determined by using 〓 and 〓 conventioanl method,DEPTtechnique,homo- and heteronuclear chemical shift correlation 2D-NMR,2D-J resolved NMR,heteronuclear relayed coherence transfer 2D-NMR,and relaxation time measurement.The assignment of resonances for 〓and 〓 were confirmed.

对中国生漆含量最大的关键漆酚组分，在〓常规NMR谱和DEPT编辑谱的基础上，采用同核及异核化学位移相关二维谱，同核J分辨谱和异核中继相干传递二维谱等2D—NMR方法，并结合弛豫时间〓测定技术等，完全确定了占中国生漆总量60%左右的有两双键共轭的三烯漆酚的结构与构型。

Methods Lymphocytes with different MTHFR C677T genotypes from the donors were cultured for 9 days in media with different concentrations of folic acid and the frequencies of micronucleated binucleated cell was evaluated by cytokinesisblock micronucleus cytome assay.

采用9天淋巴细胞长期培养辅以胞质阻断微核细胞组分析，分析不同浓度的叶酸对三种MTHFR C677T基因型乳腺癌人群和健康人群双核细胞微核率的影响与差异。

The catalytic activity of the mono- and bi-nuclear complexes, and unsymetric complex 〓〓 MeNTB =tris ( N-methylbenzimidazol-2-ylmethyl amine and tetranuclear complex 〓L = N, N, N', N'-tetrakis (N-methylbenzimidazol-2-ylmethyl-1,4-diaminobutane for oxidation of hexane was examined. Thedi-iron complexes with one exchangeable monodentate ligand and unsym-metric(μ-carboxylato) diiron complex exhibit high catalytic ac-tivity, while mononuclear Fe complex are inactive.

这些单核、双核化合物、不对称化合物〓-三氨和四核化合物〓四-1，4-丁二胺催化氧化环己烷的催化活性被测试，结果表明含有可交换配体氧桥联双核Fe化合物和不对称双核Fe化合物表现很高的催化活性，而单核的Fe化合物是非催化活性的。

By use of site mutation strategy and PCR technology, we obtained the gene P12X3C that includes full length P1, 2A, 3C and a part of 2B and 3B and the gene P12X3C3D that includes full length P1, 2A, 3C, 3D and a part of 2B and 3B. After being digested by restriction enzyme respectively, the gene P12X3C and the gene P12X3C3D were cloned into the pcDNA3. 1 and pTARGET expression vector that were digested by the same enzyme. Recombinant plasmids were checked by restriction enzyme analysis and nucleic acid sequencing. Further more, recombinant plasmids were transfected into BHK-21 cells by using lipoid. The proteins of foot-and-mouth disease virus , which were expressed in BHK-21 cells, were confirmed by sandwich-ELISA and fluoroscopy, and the capsid of FMDV was tested by electron microscope. In order to evaluate enhanced immune response of guinea pigs against FMDV, DNA vaccines which were designed to produce viral capsids lacking infectious viral nucleic acid and contained the gene P12X3C and the gene P12X3C3D were injected respectively with FMDV 3D protein which was expressed in Pichia Pastoris Secreted expression System and purified or with pcDNA3. 1/IFN which includes the gene IFN-α of cattle. Subsequently, Recombinant plasmids were injected to cattles with or without pcDNA3. 1/IFN. Anti-FMDV antibodies were detected by ELISA, and the T lymphocyte proliferation response was tested by MTT assay, neutralization antibodies titers were analyzed by micro-neutralization assay.

为研制带有O型口蹄疫病毒(Foot-and-Mouth Disease Virus，FMDV)China99株结构蛋白基因及多个非结构蛋白基因的DNA疫苗，本研究通过定点突变方法和PCR扩增方法，获得包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C以及部分2B、3B编码基因的片段P12X3C和包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C、3D以及部分2B、3B编码基因的片段P12X3C3D，将获得的基因片段直接/酶切后与同样处理的真核表达质粒连接，分别得到重组质粒pcDNA3.1/P12X3C和pcDNA3.1/P12X3C3D、pTARGET/P12X3C3D；对重组质粒进行序列测定、分析，并将重组质粒分别转染BHK-21细胞，通过双抗体夹心ELISA方法和间接免疫荧光标记方法检测细胞中FMDV抗原的表达，用电子显微镜观察病毒空衣壳的组装；为评价重组质粒作为DNA疫苗对实验动物及本动物的免疫效果，将重组质粒经肌肉注射方法接种豚鼠，并与酵母表达的纯化FMDV China99株3D蛋白及带有牛α干扰素的真核表达质粒pcDNA3.1/IFN分别/同时免疫，第二次免疫后第三周豚鼠攻以1OOID〓或1000ID〓的O型FMDV China99株；随后将质粒pcDNA3.1/P12X3C、pcDNA3.1/P12X3C3D与带有牛α干扰素的真核表达质粒pcDNA3.1/IFN同时免疫牛，三周后经牛舌皮攻以10〓ID〓的O型FMDV China99株。

By use of site mutation strategy and PCR technology, we obtained the gene P12X3C that includes full length PI, 2A, 3C and a part of 2B and 3B and the gene P12X3C3D that includes full length PI, 2A, 3C, 3D and a part of 2B and 3B. After being digested by restriction enzyme respectively, the gene P12X3C and the gene P12X3C3D were cloned into the pcDNA3.1 and pTARGET expression vector that were digested by the same enzyme. Recombinant plasmids were checked by restriction enzyme analysis and nucleic acid sequencing. Further more, recombinant plasmids were transfected into BHK-21 cells by using lipoid. The proteins of foot-and-mouth disease virus, which were expressed in BHK-21 cells, were confirmed by sandwich-ELlSA and fluoroscopy, and the capsid of FMDV was tested by electron microscope. In order to evaluate enhanced immune response of guinea pigs against FMDV, DNA vaccines which were designed to produce viral capsids lacking infectious viral nucleic acid and contained the gene P12X3C and the gene P 12X3C3D were injected respectively with FMDV 3D protein which was expressed in Pichia Pastoris Secreted expression System and purified or with pcDNA3.1/lFN which includes the gene IFN-a of cattle. Subsequently, Recombinant plasmids were injected to catties with or without pcDNA3.1/IFN. Anti-FMDV antibodies were detected by ELISA, and the T lymphocyte proliferation response was tested by MTT assay, neutralization antibodies liters were analyzed by micro-neutralization assay.

为研制带有O型口蹄疫病毒(Foot-and-Mouth Disease Virus，FMDV)China99株结构蛋白基因及多个非结构蛋白基因的DNA疫曲，本研究通过定点突变方法和PCR扩增方法，获得包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C以及部分2B、3B编码基因的片段P12X3C和包含有FMDV China99株结构蛋白P1、非结构蛋白2A、3C、3D以及部分2B、3B编码基因的片段P12X3C3D，将获得的基因片段直接／酶切后与同样处理的真核表达质粒连接，分别得到重组质粒pcDNA3.1／P12X3C和pcDNA3.1／P12X3C3D、pTARGET／P12X3C3D；对重组质粒进行序列测定、分析，并将重组质粒分别转染BHK-21细胞，通过双抗体夹心ELISA方法和间接免疫荧光标记方法检测细胞中FMDV抗原的表达，用电子显微镜观察病毒空衣壳的组装；为评价重组质粒作为DNA疫苗对实验动物及本动物的免疫效果，将重组质粒经肌肉注射方法接种豚鼠，并与酵母表达的纯化FMDV China99株3D蛋白及带有牛α干扰素的真核表达质粒pcDNA3.1／IFN分别／同时免疫，第二次免疫后第三周豚鼠攻以100ID_(50)或1000ID_(50)的O型FMDV China99株：随后将质粒pcDNA3.1／P12X3C、pcDNA3.1／P12X3C3D与带有牛α干扰素的真核表达质粒pcDNA3.1／IFN同时免疫牛，三周后经牛舌皮攻以10~4ID_(50)的O型FMDV China99株。

She gently rebuff ed him, but agreed that they could be friends

她婉言拒绝了，但同意作为朋友相处。

If in the penal farm, you were sure to be criticized.

要是在劳改农场，你等着挨绳子吧！