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MSCs were cultured in a conditional medium in subculture including: dexamethasone, vitamin C, and β-glycerophosphate. At the same time, we constructed the eukaryotic expression vector containing VEGF165gene. VEGF165 gene was transfected into MSCs by means of LipofectAMINE?2000. The stably gene expressive cells were screened with G-418 for 14 days.

在脂质体介导下将构建成功的真核表达载体pcDNA3.1- VEGF165转染到诱导培养的MSCs,G-418筛选阳性细胞克隆,将阳性克隆细胞和未转染的MSCs共同接种于β-磷酸三钙上,体外培养7天后,植入原大鼠肌肉内。

Design of strength calibration application program for pump shaft parts;2. The calibration of parameters is a key step in the application of IWA Activated Sludge Models.

对国际水协的活性污泥法动力学模型在应用中遇到的参数校核问题进行研究,总结了目前常用的三种方法:专家法、灵敏度分析法和过程工程法。

In this study aldylencdithiomethylene Malonic acid acts as the main coordination agents and compound iron ion have been synthesized by the means of solution, As a result ,the 1D polymer is obtained.

本论文采用常规的溶液合成方法,首先设计合成了一个以含有二硫缩烯酮结构的丙二酸类配体和过渡金属铁离子的单核配合物,再以此单核配合物作为配体设计合成了三个异多核配合物和配位聚合物。

For raising the accuracy of the diagnasis ancl treatinemt of ALH We realised of 86 patients with diagnosis of Autoimmune liver disease, strictly defined by the criteria for diagnosis of autoimmune hepatitis or the results of liver histology, and tested its biochemical, serological, histological results. AIH patients were main women female 75 cases, male 11 cases, main in 40 - 60 years old ,AIH characterized by a flare of ALT, AST, elevated immunoglobulin G,γ-immunoglobulin: ALP, IgG, and presence of antinuclear antibody (81.5%); moderate or severe periportal or periseptal lymphocytic piecemeal necrosis.

为提高对自身免疫性肝炎的诊治水平,采用国际自身免疫性肝炎小组新修订的描述性诊断标准和计分系统,对符合诊断的患者进行临床、生化、病理特点分析。86例AIH患者,女75例,男11例,高发年龄为40~60岁,其中Ⅰ型80例(93.020k),Ⅱ型3例(3.49%);Ⅲ型3例(3.49%);肝功能生化检查表现为肝炎样改变;实验室检查主要表现为高γ球蛋白血症,高IgG、ALP、γ-GT及甘油三酯增高,81.5%患者抗核抗体阳性:病理改变以界板性肝炎,浆细胞浸润为主,78%的患者对免疫抑制剂治疗效佳。

The first 3D heterometal phosphonate based on polynuclear cluster with left-handed and right-handed channels has been synthesized, and the gas adsorption ability and the thermal stabilization are also investigated.

合成出首例基于多核簇的三维异金属膦酸有机框架,在此结构中具有交替的左手和右手螺旋孔道,并且研究了此有机框架的气体吸附能力以及热稳定性。

Novel rod like phenylene thiophene based polyhydroxy amphiphiles, derivatives of gallic acid combining three hydrophilic 2,3-dihydroxypropyloxy groups and one alkyl chain via central aromatic linking units, have been synthesized by using Ni, Pd(0) catalyzed coupling reaction as key steps.

以Ni, Pd (0)催化的C-C偶合反应为关键步骤合成了基于苯联噻吩的棒状型多羟基两亲性分子,它们是以棒状芳香核为中心部分,一端带三重亲水的2,3-二羟基链,另一端为带一条烷基链的没食子酸衍生物。

This work is mainly concerned with molecular magnetism of polynuclear complexes and supramolecular crystal engineering.

本论文围绕多核配合物的分子磁学和超分子晶体工程这两个最为活跃的前沿研究领域,选用大环配体tacn(1,4,7-三氮杂环壬烷)和含有外延桥的双草胺。。。

Relative contents of four main cellular proteins corresponding to MALDI-TOF-MS peaks m/z 4466, m/z 6455, m/z 6514, and m/z 7772 were all found to be close correlated with the concentration of melamine, although their up- and/or down-regulations showed different patterns when the concentration of melamine changed. Support vector regression leave-one-out method, with the optimized polynomial kernel function and a poly-degree of 3, was chose to evaluate the correlation between the alteration of Tetrahymena pyriformis main proteins'relative contents and the concentration of melamine in the culture medium.

支持向量机软件中支持向量回归support vector regressi(来源:A3cBC17论b1文网www.abclunwen.comon, SVR留一法预报结果表明,选用幂指数为3的多项式核函数进行建模,相对误差的平均值为7.238%,说明梨形四膜虫主要蛋白质相对含量的变化与培养基中三聚氰胺浓度变化相关。

Through the observation via scanning electron microscope,we can discover that chips mainly have three types of micro-shapes like acicular,massive and squamose,its formation corresponds to PBX macro-fracture which caused by the nucleation and growth of crack and mainly embodied as transgranular fracture of explosive particles.

利用SEM观测发现PBX切屑主要有针状、块状和鳞片状三种细观形态,切屑的形成体现为裂纹的成核扩展导致的宏观断裂,并主要表现为炸药颗粒的穿晶断裂。

Using the hypoxic brain model, we expect this three-year project will give us answer about the value of Tc-99m HL 91 as a hypoxic marker, and will provide us better understanding of the uptake mechanism of Tc-99m HL 91 at the tissue, cell, and subcell level.

因此吾人预计此三年计画的研究成果将可以藉由脑部缺氧模式来证实及确认Tc-99m HL 91是否为一个具有潜力的侦测缺氧核医药物;除此之外,更希望对於Tc-99m HL 91在组织、细胞及次细胞层次的作用机制能够加以明了。

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However, as the name(read-only memory)implies, CD disks cannot be written onorchanged in any way.

然而,正如其名字所指出的那样,CD盘不能写,也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口,替代木托盘,免薰蒸,符合欧盟、北美各国对出口货物包装材料的法令要求;喷涂钢托盘适用于重载上货架之用,托盘表面根据需要制作成平板状、波纹状及间隔形式,满足不同的使用要求。

A single payment file can be uploaded from an ERP system to effect all pan-China RMB payments and overseas payments in all currencies.

付款指令文件可从您的 ERP 系统上传到我们的电子银行系统来只是国内及对海外各种币种付款。