三度法

This experiment with dilute alkali swelling on collagen extraction and separation, then purified by gel from collagen, and then glutaraldehyde, carbodiimide, has three types of di-isocyanate cross-linking agent on the plastic recombinant protein molecules cross-linked, the final test of infrared spectroscopy analysis of the results of their cross-linking to determine the extent of the effectiveness of cross-linking cross-linking agent to have an initial understanding.

本实验用稀碱溶胀法对胶原蛋白进行提取与分离，接下来用凝胶层析纯化所得胶原蛋白，然后用戊二醛、碳化二亚胺、己二异氰酸酯三种交联剂对胶原蛋白分子进行重组交联，最后通过对红外光谱测试结果的分析，对其交联程度进行判断，对交联剂的交联功效有一个初步的了解。，谢谢。

There are a weight index , physique percentage mainly and spend three kinds fatly to point out the present fat diagnostic standard; The fat endangers the middle-aged and old health seriously, many kinds of diseases relate to the fact that fat ; The fat reason to produce has inherent cause, physiological factor, psychological factor, social environmental factor, bad eating habit factor and factor related to movement,etc. mainly; Have probe into advantage that oxygen keeps fit through sports of having, has combined the middle-aged and old characteristic and proposed the middle-aged and old weight-reducing movement prescription , put forward the nutrition with method of weight-reducing of oxygen movements to intervene at the same time .

指出目前肥胖的诊断标准主要有体重指数、体质百分数和肥胖度三种；肥胖严重危害中老年的健康，多种疾病与肥胖有关；肥胖产生的原因主要有遗传因素、生理因素、心理因素、社会环境因素、不良饮食习惯因素及与运动有关的因素等；探讨了有氧运动减肥的优点，并结合中老年特点提出了中老年减肥运动处方，同时提出了有氧运动减肥法的营养干预。

We chose 30 type milli-needle as tools after routine sterilization. Usually we pick levelly 0.5-0.8 inch at Baihui, 1. 5 inch at Jiaji, 1-1. 5 inch inclinedly to the nose tip at Fengchi, 1-1. 5 inch inclined at Jianjin, 1-1. 5 inch at Shousanli. After obtaining gas by rotating method, we used moxibustion at Baihui by lighting the moxa strip, and put the acupuncture and moxibustion therapeutic instrument (made in shanghai, G6805-Ⅰ) at other acupuncture points. We chose thin and dense wave and adjust the current strength according to endurance of the patients, and 20 minutes each time, one time every other day, 15 times a course.

操作：患者俯卧位或坐位，选30号毫针，常规消毒，管筒进针，进针后百会穴平刺0.5～0.8寸，夹脊穴直刺1.5寸，风池穴针尖微下，向鼻尖斜刺1～1.5寸，肩井斜刺1～1.5寸，手三里直刺1～1.5寸，行捻转法得气后，将艾条点燃后于百会穴上行温和灸，其余各穴接上海产G6805-Ⅰ型针灸治疗仪，用疏密波，电流强度以病人能耐受为度（其中风池穴的电流强度以偏小为宜），每次20分钟。

Then, nanoparticles were prepared with a reprecipitation method and tested. We found that the nanoparticles with small size and lower poly-dispersion index can be prepared though shortening deposited time, decreasing concentration, increasing deposited temperature and adding surfactant.

利用二次沉淀法制备出三种有机分子不同粒径的纳米微粒，通过改变时间、浓度、温度、表面活性剂等实验条件分析这些因素对纳米微粒粒度的影响，发现缩短放置时间、减少有机稀溶液的注入量、升高放置温度、加入表面活性剂可以得到小粒度，低分散度的纳米微粒。

According to the results of the "Sociometric Method", the number of those autistic children being welcomed by their classmates did not increase; on the contrary, the number of those being rejected did increase.

三、根据「双向度社交计量法」所得之结果，自闭症儿童在班上被喜爱的人数未增加，被拒绝的人数增加，其在班上的社会地位未提升。

EDTA was used as masking agent, and trisodium citrate as total ion strength adjustment buffer was added to stabilize the ionic activity coefficient. The pH was controlled at about 5, the results were directly obtained by Gran graphing method.

以EDTA做掩蔽剂，加入柠檬酸三钠为总离子强度调节剂，使离子活度系数相对稳定，pH值控制在5左右，用Gran氏作图法直接查得结果。

Elastic modulus of specimen processed by PPT method and HP method are obtained by adopting three-point bend test and tensile test of unnotched specimen respectively.

采用无缺口试件的三点弯曲试验，得出PPT法制备的试件的弹性模量；通过柔度变化率曲线上的第一转折点得出基体的破坏应变，并由此得到基体的强度。

Some referential conclusions were drawn that the subsystems of forest resources and environment lie in weak sustainable development state expect the economy subsystem stands in a below-medium sustainable development, and the coordination development degree of the three subsystems is obviously low. So the countermeasures were proposed for Jiangsu province.

本文应用基于模糊一致矩阵的层次分析法得出了如下结论：江苏省FEES处于弱可持续发展状态，除了经济子系统处于中等偏弱的持续发展状态外，森林资源子系统、环境子系统也处于弱持续发展水平，三个子系统之间的协调发展度也明显不足。

Based on the thorough study of sources and characteristics of the three errors in laser collimation technology, an error correction method by integrating multi-point detection, multi-variant polynomial step-by-step regression analysis and adaptive noise-elimination is proposed. The influences of beam drift, bending and whipping are taken into consideration to enhance the stability of plumb datum line.

在深入分析激光准直技术中三项主要误差因素来源及其特性的基础上，提出了利用多点监测、多元多项式逐步回归分析法与自适应除噪技术相结合的综合修正方案，建立铅垂基准线的修正模型，综合考虑了激光漂移、光线弯曲以及随机抖动等因素的影响，从而有效提高了铅垂基准线的稳定度。

Depend on the amount treat quest;Filter a spoon and put a screen the small dish of the program;Cup set;Sugar server;Milk Zhong bottle;The 3 F dessert dish;Teaspoon;(put of teaspoon exactitude the method be to become 45 degrees Cape with cup)Seven 吋 personal dessert dish;Tea knife;Eat the fork of cake;Put the bowl of tea residue;Table napkin;A fresh flowers;Cozy;The wood drag along dish.

视招待客人的数量而定；滤匙及放筛检程式的小碟子；杯具组；糖罐；奶盅瓶；三层点心盘；茶匙（茶匙正确的摆法是与杯子成45度角）；七吋个人点心盘；茶刀；吃蛋糕的叉子；放茶渣的碗；餐巾；一盆鲜花；保温罩；木头拖盘。

The reasons of iron ions content overproof in grade Ⅱ desalting water system,such as variation water quality,contamination of regenerant , operation adjustment of pretreatment system and switching operation of bed were discussed.

对二级脱盐水系统中铁离子含量超标的原因，如来水水质发生波动、再生剂受到污染、预处理系统操作调整、床体运行切换等进行了论述。

You were hired to drum up new business, so go and do it.

公司雇你招徕新业务，你就做你的事好了。