三体细胞

The three types of reconstructed embryos(reconstructed embryo of goat-rabbit, goat-bovine and goat-goat) were produced respectively by nuclear transfer using goat ear firbroblast cells as donors and rabbit, bovine and goat ooctyes as recipients, and the factors of cytoplast environments influencing cloned embryo development in vitro, cytoplast effect in nuclear transfer, the methods of transferring cloned embryo and the effects of pregnancy factor on implantation of cloned embryo were studied in this experiment, in order to supply basis for solving the difficulty of implantation of inter-species cloned embryo in recipient and improving the efficiency of cloning.

本试验以波尔山羊耳成纤维细胞为供体，分别以牛、羊、兔的卵母细胞为受体进行体细胞核移植，构建了山羊-兔、山羊-牛和山羊-山羊三种重构胚。研究了不同的胞质环境对重构胚体外发育的影响与核移植中的胞质效应，并对异种克隆胚胎采用了去透明带移植和配种后再移植的方法，探讨移植方法和妊娠因子对异种克隆胚胎在受体动物体内发育的影响，旨在为解决异种克隆胚移植不易着床发育的难题和为提高动物克隆效率提供理论依据。

Seed explants of Eleutherococcus senticosus produced somatic embryos directly ongrowth regulator-free medium. The results were showed that use low temperature stressmethod to relieve the Eleutherococcus senticosus seed conformation dormancy andphysiological dormancy, to induce seed germinate. Proliferation of Eleutherococcus senticosussomatic embryo without any plant growth regulathors was better in liquid medium containing1/3MS+sucrose 3%, and better in solid medium containing 1/3MS+sucrose 1%.Eleutherococcus senticosus secondary embryo was stick to matrix through espial of cytology.Eleutherococcus senticosus somatic embryo was cultivated some times to form immature frondin liquid medium, then transfer into solid medium to form full plant. The experiment wasindicated that much more Eleutherococcus senticosus immature frond can be cultured inbioreactor, so large-scale production in bioreactor culture can be done.

研究结果表明：低温胁迫处理刺五加种子可以打破种子的形态休眠和生理休眠，促使种子发芽：在不添加任何植物激素的培养基中诱导刺五加体胚形成，其中，1／3MS+3％蔗糖的培养基搭配有利于液体培养的刺五加体胚增值，1／3MS+1.0％蔗糖的培养基搭配有利于固体培养的刺五加体胚增值；在进行体细胞胚增殖时，体细胞胚继代三周后表面开始形成很多次生胚，对刺五加次生胚进行细胞学观察，发现刺五加次生胚与母体只是粘贴在一起；刺五加体胚需在液体培养基中培养一段时间形成幼植物体，实验表明可以通过生物反应器培养大量的刺五加幼植物体，然后转入固体培养基中形成完整植株，因此可以通过生物反应器培养进行规模化生产。

One of two cylindrical structures that are composed of nine triplet microtubules and the asters during mitosis .

由九个三联微管组成的两个圆柱体细胞组织之一，在有丝分裂中形成星状

We also analysed the SCs of 30 spermatocytes at pachytenes, showing 20 autosomal bivalents, a quadrivalent 11; 18 and a sexual bivalent.

病例4为受孕浪费、生育过一例具有21三体特征患儿的患者，体细胞染色体分析表明，其核型为45，XY，-21，-21，+rob（21q；21q）。

One of two cylindrical cellular structures that are composed of nine triplet microtubules and form the ''.

中心体由九个三联体微管组成的两个圆柱体细胞组织之一，在有丝分裂中形成星状体

One of two cylindrical cellular that are composed of nine triplet microtubules and the asters during mitosis .

中心由九个三联微管组成的两个圆柱体细胞组织之一，在有丝分裂中形成星状

One of two cylindrical cellular structures that are composed of nine triplet microtubules and form the asters during mitosis.

中心体由九个三联体微管组成的两个圆柱体细胞组织之一，在有丝分裂中形成星状体

Studies on the Photosynthesis of Several Trisomics in Chinese Cabbage Brassica campestris ssp. pekinensis (Rupr. Olsson;3. The karyotype of triplo 6 of indica IR36 trisomic is analysized, and the additional chromosome is inentified.

本研究以籼稻IR36第六号三体（triplo6）为材料，进行了水稻体细胞核型分析，同时鉴定验证了三体材料，并为水稻标准核型图的建立提供了一些依据。

So in this paper,firstly the law of somaclonal variation of Lilium concolor,L.regale and L.henryi were investigated systematically from three ways, including Morphology,cytology and molecular biology.Secondly,breeding heat-resistant cultivars for Lilium regale was carried out.

因此本研究从体细胞无性系变异规律入手，选取了耐热性较好的原生种渥丹、岷江百合和湖北百合，从形态学、细胞学和分子生物学三方面进行了较为系统的研究，并在此基础上对岷江百合开展了体细胞无性系耐热选育工作。

This experiment selecetd apex , flower bud and radicle of Camellia sinensis cv. Longjing 43 as research objects picked from the tea garden of Anhui Agricultural University. In this experiment ,three kinds of Chromosomal techniques were used ,including conventional sheeting method , disassociating cell wall hypoosmotic method and macrocoils method.

本试验以安徽农业大学茶园中龙井 43 的芽尖、花蕾以及其茶籽培养的幼根为研究对象，采用传统的压片法、去壁低渗法、风油精法三种制片方法，进行系统的比较和分析，这三种方法均能找到茶树体细胞有丝分裂相。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失