三代的

Profess signal element and prevented the decline in efficiency that so commonly spoiled the fortunes of family firms in the second and third generation after the energetic founders.

这种效率的降低在精力充沛的创业者之后的第二代和第三代人的时候，经常会毁掉那些家族公司的财富。

The change met the technical requirements of the new age by engaging a large profess signal element and prevented the decline in efficiency that so commonly spoiled the fortunes of family firms founders.

这种效率的降低在精力充沛的创业者之后的第二代和第三代人的时候，经常会毁掉那些家族公司的财富。

The new age by engaging a large profess signal element and prevented the decline in efficiency that so commonly spoiled the fortunes of family firms in the second and third generation after the energetic founders.

这种效率的降低在精力充沛的创业者之后的第二代和第三代人的时候，经常会毁掉那些家族公司的财富。

The change met the technical requirements of the new age by engaging a large profess signal element and prevented the decline in efficiency that so commonly spoiled the fortunes of family firms second founders.

这种 效率的降低在精力充沛的创业者之后的第二代和第三代人的时候，经常会毁掉那些家族公司的财富。

The change met the technical requirements of the new age by engaging a large profess signal element and prevented the decline in efficiency that so commonly spoiled the fortunes of family firms the founders.

这种效率的降低在精力充沛的创业者之后的第二代和第三代人的时候，经常会毁掉那些家族公司的财富。

Methods: A 5ml bone marrow was extracted from the lilac of human volunteers. By Percoll fluid and density gradient centrifugation, the MSC was obtained; after the cells filled the bottom of vessel, subcultured them, when they subculture in third generation, redigested them, 500 R/min centrifugate, alter the completed medium to chemical definition medium, examined the form change and prolifration of cells by invert microscope, toluidine blue stain、immunocytochemical stain and RT-PCR to test the type Ⅱ collagen mRNA and proteoglycan.

取健康成人髂后上棘处骨髓5ml，经percoll液分离后密度梯度离心，〓／ml密度接种培养，观察原代细胞的贴壁、增殖状况，细胞长满瓶底后进行传代培养；传至第三代细胞，重新消化后以500转／分钟轻度离心5分钟，〓／ml接种，改用化学限定培养基代替完全培养基培养，倒置显微镜观察细胞生长情况及形态变化，甲苯胺蓝染色观察诱导细胞合成细胞外基质中的蛋白多糖，免疫细胞化学染色检测ECM中Ⅱ胶原的蛋白合成，RT-PCR鉴定诱导细胞Ⅱ胶原mRNA的表达。

The aim are:lTo examine the proliferation ability and potential chondroblast differentiation ;2To find an ideal condition stimulated BMSC differentiate into chondroblast;3To examine the chondroblast proliferation in porous scaffolds and to explore the interaction between cells and materials;4To examine the release of cytokine in vitro.

取健康成人略后上棘处骨髓 5ml，经 percoll液分离后密度梯度离心，10'砌l密度接种培养，观察原代细胞的贴壁、增殖状况，细胞长满瓶底后进行传代培养；传至第三代细胞，重新消化后以500转／分钟轻度离心 5分钟，10V加 l接种，改用化学限定培养基代替完全培养基培养，倒置显微镜观察细胞生长情况及形态变化，甲苯胺蓝染色观察诱导细胞合成细胞外基质QCM）中的蛋白多糖，免疫细胞化学染色检测ECM中＊胶原的蛋白合成，RT干CR鉴定诱导细胞＊胶原mRNA的表达。

Naphthalene superplasticizer compared with other second-generation, third-generation POLYCARBOXYLIC superplasticizer With the improvement of quality of performance and quality.

与萘系等第二代减水剂相比，第三代聚羧酸系高效减水剂的性能与质量有了质的提高，基本能够满足高性能混凝土对减水剂的性能要求，该类产品基本具备了取代萘系减水剂的技术性能优势与经济条件。

Naphthalene superplasticizer compared with other second-generation, thellord-generation POLYCARBOXYLIC superplasticizer With the improvement of quality of performance and quality.

与萘系品级二代减水剂比拟，第三代聚羧酸系高效减水剂的机能与质量有了质的提高，基本能够餍足高机能混凝土对于减水剂的机能要求，该类产物基本具有了代替萘系减水剂的技术机能优势与经济前提。

To have the level of fourth-generation technology, thanks to good fortune by the German-made automatic winding machine AC338 for example, with the third-generation type of comparison, the practical winding speed of nearly 30 percent, most can be as high as 2 000 m / min;筒纱quality increased yarn Uster CV value of the two machines are basically the same though, but the quality of yarn, such as details of 1000 meters from 14.5 to 12, 17.24 percent lower; km thick section from 134.3 to 121.7 , 9.38% lower; Neps kilometers from 121.6 to 115.5, lower 5%; 3 mm in length Hairiness (months / 10m) from 135 down to 102, reduced 24.4%; 14.36% increase in production; production efficiency 8.5%, not筒纱the use of high-quality, the following procedures brought about by economic interests.

以具有第四代技术水平的，由德国赐来福公司制造的AC338型自动络筒机为例，与第三代型机比较，实用卷绕速度提高了近30%，最可高达2 000 m/min；筒纱质量大幅度提高，乌斯特条干CV值两机虽然基本相同，但纱的质量如千米细节由14.5降至12，降低了17.24%；千米粗节由134.3降至121.7，降低了9.38%；千米棉结由121.6降至115.5，降低了5%；3 mm长度毛羽(个/10m)也由135降到102，降低了24.4%；产量提高14.36%；生产效率提高8.5%，这还不算使用高质量的筒纱后，后道工序所带来的经济利益。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。