丁酸

To elucidate the possible mechanism of differentiation and/or apoptosis in NB4, K562 cells induced by tributyrin, a histone deacetylase inhibitor , the level of acetylated histone H3 was detected by Western blot, p21(superscript WAF1) expression was detected by semi-quantitative RT-PCR.

为了探讨组蛋白去乙酰化酶抑制剂三丁酸甘油酯(tributyrin， TB)诱导NB4、K562白血病细胞分化和凋亡的作用机制，利用Western blot方法及逆转录聚合酶链反应检测TB作用前后NB4、K562细胞组蛋白H3乙酰化水平以及p21(上标 WAF1)表达量的改变。

The activity of lipasc from S78- pVT102U/a -lip was up to 20U/ml after 72h fermentation; The product of S78-pVT102U/a-L-lip07 was little, only formed the halo on the tributyrin plate.

其中，重组酵母S78-pVT102U／α-lip摇瓶发酵72h，酶活可达20U／ml；S78-pVT102U／α-L-lip07表达的脂肪酶很少，表达产物仅在三丁酸甘油酯平板上形成透明圈。

The recombinant pET-Lip vector was transformed into E.coliBL21 and induced to express by 1mmol/L IPTG at 37℃. An expected 80kDa fusion proteinwas expressed. SDS-PAGE analysis showed the fusion protein located in the supernatant ofbacteria lysate by sonication. The fusion protein was purified by HisTrap~ HP Kit and coulddegrade tributyrin.

该菌株经IPTG诱导可表达分子量约80 kDa的融合蛋白，SDS-PAGE分析表明融合蛋白位于菌体超声裂解后的上清中，用蛋白纯化试剂盒HisTrap~ HP纯化后得到单一的目的蛋白，约80 kDa，且纯化的融合蛋白具有脂酶活性，可以分解三丁酸甘油酯。

One integrative transformant was obtained through PCR and phenotype identification, designated as A.oryzae ONL1. The 7-day fermentation broth of A.oryzae ONL1 could hydrolyze tributyrin verified by forming transparent cleared zone on tributyrin plate, and the enzyme activity reached 2.5 U/mL. The SDS-PAGE electrophoretic map of the fermentation broth demonstrated that there was a typical RML band at 32.5 kDa.

其培养7天的培养液上清在以三丁酸甘油酯为底物的平板上形成清晰的水解透明圈，碱滴定法酶活测定表明培养液酶活可达2.5 U/mL，培养液上清的SDS-PAGE图谱在32.5 kDa处有RML的特征条带。

The results were summarized by variance analysis and correlation analysis, the diversity reached significant positively correlated level of varieties carotene, polysaccharide,γ-am1nobutyric and crude fibre content, but the diversity bad- not reached significant positively correlated level of varieties Zn, Cr and Trigonelline content.

对11个品种肉用印度南瓜主要果实性状进行调查，并对果肉的8种营养成分测定；通过方差和相关分析得出结论：胡萝卜素、多糖、淀粉、γ-氨基丁酸、粗纤维含量在各个品种间差异均达到极显著，Zn、Cr、葫芦巴碱的含量在各个品种间差异不显著。

With regard to bioactive components, rutin, quercetin and γ-aminobutyic acid were detected in husked buckwheat bread and unhusked buckwheat bread whereas those components were not found in white bread.

在机能性成分方面，带壳荞麦土司及去壳荞麦土司皆测出芸香苷、槲皮素及γ-胺基丁酸之成分，而白土司并不含有此三种成分。

Objective:To study the changes of excitatory and inhibitory amino acid content in hippocampus of pentetrazole-induced epileptic rats treated with volatile oil of Valeriana officinalis L,and explore the possible antiepiletic mechanism.

谷氨酸是中枢神经系统主要的兴奋性氨基酸之一，可以导致神经细胞兴奋性增加和细胞损害，在癫痫的发病机制中有着重要作用，γ-氨基丁酸则是主要的抑制性神经递质。

A method for the direct determination of auxins, indole acet ic acid,indole-butyric acid and naphthylacetic acid, by gas c hromatography with wide-bore capillary column is described.

建立了一种采用530 μm大口径毛细管色谱柱、不经衍生化处理而直接测定吲哚乙酸、吲哚丁酸和萘乙酸等植物生长素的气相色谱分析方法。

The structure inside the cells of trains A2 and A5 changed clearly in different cultural conditions. PHB(poly β hydioxybutyric acid) was convolved in A2 and this material has poteintial value in the future.

经JEOL 1 0 0CX透射电镜观察，A2、A5菌在不同培养条件下细胞内部结构发生了明显改变。A2菌细胞核中含有的聚β羟基丁酸，具有巨大的应用价值和开发潜力

There were 4 replicates of 8 pigs in every replicate.

丁酸钠的添加浓度分别为0、1和2 g/kg。

Do you know, i need you to come back

你知道吗，我需要你回来

Yang yinshu、Wang xiangsheng、Li decang,The first discovery of haemaphysalis conicinna.

1〕 杨银书，王祥生，李德昌。安徽省首次发现嗜群血蜱。