丁酯酶

Objective The aim was to study the feasibility of catalyzing the transesterification of 3-N-butylphthalide by lipase in nonaqueous media.

研究在非水相介质中脂肪酶催化丁基苯酞的转酯化可行性。

The most common methods used for screening of lipase-producing strains are observation of transparent circle on tributyrin agar plate and color-changing circle on olive oil agar plate.

在脂肪酶产生菌的筛选中，最常用的方法是三丁酸甘油酯琼脂平板透明圈法和橄榄油琼脂平板变色圈法。

The recombinant pET-Lip vector was transformed into E.coliBL21 and induced to express by 1mmol/L IPTG at 37℃. An expected 80kDa fusion proteinwas expressed. SDS-PAGE analysis showed the fusion protein located in the supernatant ofbacteria lysate by sonication. The fusion protein was purified by HisTrap~ HP Kit and coulddegrade tributyrin.

该菌株经IPTG诱导可表达分子量约80 kDa的融合蛋白，SDS-PAGE分析表明融合蛋白位于菌体超声裂解后的上清中，用蛋白纯化试剂盒HisTrap~ HP纯化后得到单一的目的蛋白，约80 kDa，且纯化的融合蛋白具有脂酶活性，可以分解三丁酸甘油酯。

The preparation of vanillin with clove oil as the substrate and soybean lipoxygenase as the bio-catalyst has been carried out in a silicon rubber membrane bioreactor.

在硅橡胶膜生物反应器系统中用丁香油为原料和大豆酯氧化酶为催化剂，进行了生物转化制备香草醛的实验研究。

The esterification reaction kinetics of hexanoic acid with butanol, catalyzed by Novozym 435 lipase, was studied in water-in-oil microemulsions formed by alkyl polyglucoside C10G1.54/n-butanollcyclohexane/water buffer solution.

研究了Novozym435脂肪酶在烷基聚葡糖苷形成的W/O微乳液体系中，催化正丁醇与正己酸合成酯的反应动力学。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。