丁酯酶

Enzymatic synthesis of isoamyl valerate in nonaqueous medium

由根霉PW 358经固态发酵生产脂肪酶，以此酶为催化剂，在非水介质中合成了丁酸异戊酯。

Aim To design and synthesize a series of novel γ-butyrolactone derivatives with FAS inhibition activities, C75 as the lead compound.

摘要目的以C75为先导化合物，设计、合成γ-丁内酯类衍生物，并研究其脂肪酸合成酶（fatty acid synthase， FAS）的抑制活性，以期发现新颖的FAS抑制剂。

Eight lipase-producing bacterium strains were screened from 40 cold-adapted strains, these strains isolated from the mud of Parece Vela sea basin in the Pacific Ocean of 5010m deep, of which two similar strains have the highest lipolytic activity and are positive to olive oil.

通过三丁酸甘油酯平板法从太平洋帕里西维拉海盆5010m的底泥中共筛出八株可产脂肪酶的菌株，其中的两株生理生化特征极其相近的菌脂肪酶活性最高，并且对橄榄油平板产生荧光。

The aim was to study the feasibility of catalyzing the transesterification of 3-N-butylphthalide by lipase in nonaqueous media.

［目的］研究在非水相介质中脂肪酶催化丁基苯酞的转酯化可行性。

However, the recombinant yeast could not grow on the traditional tributyrin plate because this kind of medium is lack of essential nutrients for yeast growth.

而传统的三丁酸甘油酯平板缺乏有效营养成分，重组酵母无法生长；橄榄油平板灵敏度较低，低脂肪酶活力的重组酵母不能产生变色圈，这给产脂肪酶基因工程菌的鉴定带来了困难。

To elucidate the possible mechanism of differentiation and /or apoptosis in NB4, K562 cells induced by tributyrin, a histone deacetylase inhibitor, the level of acetylated histone H3 was detected by Western blot, p21~ WAF1expression was detected by semi-quantitative RT-PCR.

为了探讨组蛋白去乙酰化酶抑制剂三丁酸甘油酯(tributyrin，TB)诱导NB4、K562白血病细胞分化和凋亡的作用机制，利用Westernblot方法及逆转录聚合酶链反应检测TB作用前后NB4、K562细胞组蛋白H3乙酰化水平以及p21WAF1表达量的改变。

To elucidate the possible mechanism of differentiation and/or apoptosis in NB4, K562 cells induced by tributyrin, a histone deacetylase inhibitor , the level of acetylated histone H3 was detected by Western blot, p21(superscript WAF1) expression was detected by semi-quantitative RT-PCR.

为了探讨组蛋白去乙酰化酶抑制剂三丁酸甘油酯(tributyrin， TB)诱导NB4、K562白血病细胞分化和凋亡的作用机制，利用Western blot方法及逆转录聚合酶链反应检测TB作用前后NB4、K562细胞组蛋白H3乙酰化水平以及p21(上标 WAF1)表达量的改变。

The activity of lipasc from S78- pVT102U/a -lip was up to 20U/ml after 72h fermentation; The product of S78-pVT102U/a-L-lip07 was little, only formed the halo on the tributyrin plate.

其中，重组酵母S78-pVT102U／α-lip摇瓶发酵72h，酶活可达20U／ml；S78-pVT102U／α-L-lip07表达的脂肪酶很少，表达产物仅在三丁酸甘油酯平板上形成透明圈。

One integrative transformant was obtained through PCR and phenotype identification, designated as A.oryzae ONL1. The 7-day fermentation broth of A.oryzae ONL1 could hydrolyze tributyrin verified by forming transparent cleared zone on tributyrin plate, and the enzyme activity reached 2.5 U/mL. The SDS-PAGE electrophoretic map of the fermentation broth demonstrated that there was a typical RML band at 32.5 kDa.

其培养7天的培养液上清在以三丁酸甘油酯为底物的平板上形成清晰的水解透明圈，碱滴定法酶活测定表明培养液酶活可达2.5 U/mL，培养液上清的SDS-PAGE图谱在32.5 kDa处有RML的特征条带。

The esterification reaction and itsinfluencing factors of hexanoic acid with n-butanol, catalyzed by lipaseNovozym100T, were studied in water-in-oil microemulsions formed by alkylpolyglucoside C_(12)G_(1.50)/n-butanol/cyclohexane/water system.

研究发现，十二烷基聚葡糖苷C_(12G_(1.50)／正丁醇／环己烷／H_2O体系形成的油包水微乳液中，脂肪酶Novozym100T催化正己酸和正丁醇的酯合成反应，反应初始速率V_0随正己酸的初始浓度C_~0增大而增大；随正丁醇的初始浓度C_~0的增大而减小，后V_0进入一&坪区&。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。