一转

RESULTS A eukaryotic expression system for high expression humanmutantCD59 were successfully set up : The recombinant PALTER-MAX plasmid containing human mutantCD59 cDNA and PCDNA plasmid were co-transfected into CHO cell by cation lipoid mediating method ;and the cells were grown in F12 medium containing 400ug/ml G418 for 14 days, positive clones were grown in RPMI1640 medium to get stable expressing cell lines . Highly expressing clones were selected by flow cytometry ,and were named PALTER-CD59-CHO1PALTER-CD59-CHO2 . Flow cytometry indicated that expression rates of PALTER-CD59-CHO1 and PALTER-CD59-CHO2 were 53.7%and 54.5%. Further more, Stable highly expressing CHO cell lines were more detected by immunocytochemistry and immunofluorescence technology . PALTER-CD59 -CHO1 and PALTER-CD59-CHO2 were grown in RPMI1640 to get a large of cells . CD59 protein were obtained by spalling PALTER- CD59- CHO1 and PALTER - CD59 - CHO2 cells . Stable highly expressing cells were further validated by SDS-PAGE, immunoblot analysis and solid enzyme immunoassay . PALTER - CD59 - CHO1 and PALTER - CD59 - CHO2 were glycated in RPMI1640 of 50mM ribose for 72 hours .BCECF releasing test indicted that the releasing rate of PALTER-CD59-CHO1 or PALTER-CD59-CHO2 was less high than PALTER-CHO ,and the releasing rate of glycated PALTER-CD59 -CHOI or PALTER-CD59-CHO2 was higher than unglycated ones . PALTER -CD59-CHO1 and PALTER -CD59 -CHO2 were glycated in RPMI1640 of 50mM ribose for 72 hours .BCECF releasing test indicted that the releasing rate of PALTER - CD59 - CHOI or PALTER - CD59 - CHO2 was less high than PALTER-CHO ,and the releasing rate of glycated PALTER-CD59-CHO1 or PALTER - CD5 9-CHO2 was higher than unglycated ones .

结果 成功构建突变人CD59的真核细胞表达系统：运用阳离子脂质体介导法将含有突变人CD59的PALTER—MAX重组质粒与PCDNA共转染入CHO细胞：用含有400ug／mlG418的F12培养基培养14天，筛选出稳定阳性表达克隆，RPMI1640培养基扩增获得稳定表达细胞株，并用流式细胞术进一步筛选出高效表达细胞株分别命名为PALTER—CD59—CH01、PALTER—CD59—CH02，表达率分别为53.7％、54.5％；应用免疫组化方法、免疫荧光技术进一步鉴定阳性细胞株；RPMI1640培养基大量扩增PALTER—CD59—CH01、PALTER—CD59—CH02细胞株，裂解细胞得到CD59蛋白质；通过SDS—PAGE凝胶电泳技术、免疫印迹技术、固相酶联免疫吸附试验验证了这两中文摘要个阳性细胞株CO59蛋白的高效表达；50mM核糖培养72小时，获得突变人CD59糖化细胞株，BCECF染料释放试验结果显示，PALTER一CD59一CHOI、pALTER一CD59一CHOZ细胞较PALTER一CHO细胞染料释放率低，未糖化PALTER一CD59一CHOI、PALTER一CD59一CHOZ细胞比较糖化后细胞染料释放率低。

Gaits and movements performed at this level include collected and extended walk, trot, and canter; trot and canter half-pass ( a movement where the horse travels on a diagonal line keeping its body almost parallel with the arena wall while making bothe forward and sideways steps in each stride); passage ( a slow-motion trot); piaffe (an approach to "trot in place"); one and two tempi changes (where the horse changes from one lead to the other in the canter); and pirouettes (a 360-degree circle that is almost in place).

这一级别比赛所要表现的马的步法和动作包括自然而专注的慢步、快步和跑步；快步和跑步的半斜横步（这一动作要求马在每一跨步完成向前方和向侧方两步的同时沿一条对角线行走，还要保持身体几乎和马场的围栏平行）；巴沙基斜横部；琵阿斐步法（一种接近"原地快步"的步法）；一至两个步调变化（要求马在跑步中从一种节奏变为另一种）；以及皮鲁埃特急转（几乎原地做一个突然起立以后腿为轴心的360°旋转）。

However, there are still several safety concerns about the use of a DNA vaccine, which include the possibility of integration into the host genome, adverse immunopathology, and anti-DNA autoantibody induction.Attempting to solve some of these problems, eukaryotic expression plasmids pTL-8/ AMA-1 and pTL-8/ AMA-1 which express trans-activator or reverse trans-activator, respectively, and AMA-1 gene of Plasmodium falciparum were constructed by using tetracycline regulable system.

首先构建了恶性疟原虫顶第四军医大学硕士学位论文端膜抗原1(AMA一1)基因和反式活化因子基因的真核表达质粒pTL一8/灿认一l和PTL一8/灿认一1，并大量制备这两种质粒及表达转录抑制子的质粒pUHS6一1。

Late Qing scholar Ouyang Yu, a contenporary of Hu Xueyan, recorded in detail of the bitter spectacle of this business war in Trivial Stories: As soon as fresh silk came into season, Hu immediately sent men to buy in, none left for foreign merchants; Being utterly hopeless, foreign merchants told Hu that they would like to add 10 million tael to buy all the silk, but Hu sticked to 12 million tael, they refused.

与胡雪岩同时代的晚清学者欧阳昱在《见闻琐录》中详细记录了这场商战的惨烈景象：其年新丝一出，胡即派人大量收购，无一漏脱，外商想买一斤一两而莫得，无可奈何。向胡说愿加利一千万两，如数转买此丝，胡非要一千二百万两不可。

Gaits and movements performed at this level include collected and extended walk, trot, and canter; trot and canter half-pass ( a movement where the horse travels on a diagonal line keeping its body almost parallel with the arena wall while making bothe forward and sideways steps in each stride); passage ( a slow-motion trot); piaffe (an approach to "trot in place"); one and two tempi changes (where the horse changes from one lead to the other in the canter); and pirouettes (a 360-degree circle that is almost in place).

这一级别比赛所要表现的马的步法和动作包括自然而专注的慢步、快步和跑步；快步和跑步的半斜横步（这一动作要求马在每一跨步完成向前方和向侧方两步的同时沿一条对角线行走，还要保持身体几乎和马场的围栏平行）；巴沙基斜横部；琵阿斐步法（一种接近&原地快步&的步法）；一至两个步调变化（要求马在跑步中从一种节奏变为另一种）；以及皮鲁埃特急转（几乎原地做一个突然起立以后腿为轴心的360°旋转）。

Photocopying with a good first tattoo patterns on the Transfer of paper did not ink the side, iron-on transfers other side of the bottom of a White Fang.

先用复印好的纹身图案纸放在转印纸没油墨一侧，转印纸另一侧下方放一白纸。

Detecting overrepresented known transcription factor binding motifs in a set of promoter sequences of co-regulated genes has become an important approach to deciphering transcriptional regulatory mechanisms.

在一组共调控基因的启动子序列中检测过代表的已知转录因子结合motifs已经成为破译转录调控机制的重要方式。

We precompute the test patterns to make it to be encoded more easily by alternation and run-length codes. The result shows that the effect is obvious after precomputating.3. Aiming at reseeding issue in the classic fold counter technology, this paper presents a method, it doesn\'t need reseeding.

针对传统的折叠计数器技术需要重新播种的问题，本文给出了一种方法，它不需要进行重新播种，当一个折叠集生成完毕之后，可以通过在若干个中间折叠集的跳转跳转到下一个折叠集。

In this example, the pilot went for two flat spin rotations but the trick can be performed with only one.

在这个例子中，放飞者走出了两圈平转但是这个技巧是允许只转一圈的。

Based on the views on metonymy held by Jakobson, Pankhurst and Gibbs, this paper mainly attempts to explore the function of conceptual metonymy in fictional narration and its interpretation from the following three aspects: characterization, description of characters, and description of scene.

人们对转喻的认识发生了彻底的改变：转喻不只是语言和修辞现象，更是认知和思维现象，它作为一种概念现象和认知过程普遍存在于人们的日常语言和思维过程中。

For a big chunk of credit-card losses; the number of filings (and thus charge-off rates) would be rising again, whether

年美国个人破产法的一个改动使得破产登记急速下降，而后引起了信用卡大规模的亏损。

Eph. 4:23 And that you be renewed in the spirit of your mind

弗四23 而在你们心思的灵里得以更新