一转

Vector to raster: Inside point diffuse method, diffuse from internal seeds within polygon to neighbor points, as far as to the boundary of polygon; Pluralism integral arithmetic, according to the close boundary of polygon, makes uncertain point calculate pluralism integral; Radial and scanning arithmetic, from a uncertain point which located outside of the figure to draw radial, according to the junction of radial and polygon to judge the internal and outside relationship; Boundary algebra filling, a vector convert to raster arithmetic which based on integral thought, suit for polygon vector convert which recorded topological relationship.

矢量转栅格：内部点扩散法，即由多边形内部种子点向周围邻点扩散，直至到达各边界为止；复数积分算法，即由待判别点对多边形的封闭边界计算复数积分，来判断两者关系；射线算法和扫描算法，即由图外某点向待判点引射线，通过射线与多边形边界交点数来判断内外关系；边界代数算法，是一种基于积分思想的矢量转栅格算法，适合于记

This paper presents the geostrophic and gradient wind appropriate calculation formulas used on facsimile surface weather chart, puts forward a new method of sea surface wind estimating from the theoretical wind speed by establishment of the wind speed correction database. Also the application characteristics and advantages of the gradient wind calculation method on severe weather system are discussed.

为了深入探讨海面风估算方法，给出了传真地面天气图上地转风、梯度风的近似计算公式，介绍了用传真图实时计算地转风与梯度风的实现方法，提出了通过建立风速订正值库由风速理论值实时估测平均海面风速的一种新方法，并深入探讨了梯度风测风方法在强天气测风中的应用特点和优势。

Is the last year that asset write-down reversals were allowed. This paper will do some special research in the implementation of it.

另外，由于2006年是允许长期资产减值转回的最后一年，本文将对我国上市公司2006年的资产减值转回情况作特别分析。

A cogging torque measurement system was proposed, which was composed of stepping motor, torque sensor, etc.

提出了一种由步进电机、转矩传感器等元件组成的齿槽转矩测试系统。

Daier that discontenting experiencing people to this nobleman family, one fine day may be done , he is unwilling to be that this family bears this responsibility , he is turned to a bourgeoisie by nobleman class, fairly more different than exploiting class turns to a proletariat.

代尔那感受到人们对这个贵族家庭的不满，总有一天会完蛋，他不愿为这个家庭承担这个责任，他由贵族阶级转到资产阶级，还不同于剥削阶级转到无产阶级。

In the benchmark model, the acceptability of debit cards is exogenously given, while it is endogenized as determined by agents' decision to acquire information.

在我们模型中，一开始我们假设接受转帐卡的机率是外生给定的，接著再由人们决定是否要成为有能力区分转帐卡的人，此部份是内生决定的。

The PSCA_3 fragment was selected for its superior expression level in eukaryotic cells.Then the sig-PSCA_3-Fc-GPI genetic fragment was cloned into pVAX1-neo-IRES-GM/B7 vector to construct the final immunological inhanced DNA vaccine pVAX1-PSCA_3-FcGB. Immunofluorescence and flow cytometry were used to confirm the expression of PSCA_3 fragment by transfected into Cos7 cell.Finally,the anti-tumor effect of pVAX1-PSCA_3-FcGB was tested in murine prostate cancer model generated by RM-1 cell line.The animal was immunized with pVAX1-PSCA_3-FcGB DNA vaccine by intramuscular injection plus electroporation,pVAX1 and pVAX1-PSCA_1-FcGB plasmid were used as control.The inhibitory effect of tumor was investigated by observion of forming time,volume and inhibition ratio of tumor.Results:DNA sequencing conformed that the heterological PSCA fusion antigen fragment which was synchronized by overlapping-extending-PCR,was consistent to design.Enzyme digestion analysis showed that the 1 to 4 copies heterological PSCA fusion antigen fragments were constructed successfully.

方法(1)检索GenBank，选择包含人主要T细胞抗原表位序列的人PSCA基因片段，应用异种化抗原设计技术，保留人T细胞抗原表位，设计异种化PSCA融合抗原片段；(2)根据核酸序列按中心模板法设计引物，应用重叠延伸PCR技术拼接合成异种化PSCA融合抗原片段基因，以PCR、限制性酶切和DNA序列测定法进行鉴定：(3)利用DNA限制性内切酶BssHⅡ和MluⅠ酶切后粘端互补的特点，采用同尾酶法构建1—4拷贝异种化PSCA融合抗原片段(PNCA_1-PSCA_4)，并将上述片段分别插入真核表达载体pCI-neo-Fc-GPI中，转染293T细胞，借助免疫荧光+流式细胞术考察插入片段表达效率，最终选定PSCA_3片段进行下一步研究；(4)将sig-PSCA_3-Fc-GPI基因片段自pCI-PSCA_3-Fc-GPI质粒上切下，插入pVAX1-neo-IRES—GM/B7载体中，构建免疫增效DNA疫苗pVAX1-PSCA_3-FcGB，并应用转染Cos7细胞+免疫荧光/流式细胞术方法鉴定其在真核细胞中的表达情况；(5)给8周龄雄性C57BL/6小鼠皮下种植RM-1细胞，制备小鼠前列腺癌模型，并采用股四头肌肌肉注射+电脉冲法(Electroporation，EP)接种DNA疫苗质粒pVAX1-PSCA_3-FcGB，同时接种pVAX1空载体质粒和pVAX1-PSCA_1-FcGB质粒作为对照，通过观察计算免疫动物的成瘤时间、肿瘤体积和抑瘤率，来评价该DNA疫苗在小鼠体内的抑瘤效果。

The CCAAT binding factor of brewer's yeast Saccharomyces cerevisiae has been shown previously to be a heteromeric complex containing HAP2, HAP3, HAP4 and HAP5 subunits. This factor can bind specifically to CCAAT-containing upstream sites within the promoters of numerous yeast genes, and then activate the transcription.

HAP转录因子(HAP2/HAP3/HAP4/HAP5)是存在于酿酒酵母中的一种异源多聚蛋白，它能与酵母中许多启动子上游的CCAAT盒专一性结合，以增强基因的转录。

The CCAAT binding factor of brewers yeast Saccharomyces cerevisiae has been shown previously to be a heteromeric complex containing HAP2, HAP3, HAP4 and HAP5 subunits. This factor can bind specifically to CCAAT-containing upstream sites within the promoters of numerous yeast genes, and then activate the transcription.

HAP转录因子(HAP2/HAP3/HAP4/HAP5)是存在于酿酒酵母中的一种异源多聚蛋白，它能与酵母中许多启动子上游的CCAAT盒专一性结合，以增强基因的转录。

Cells were transfected with lamin A/C siRNA using the transfection reagents indicated, according to the manufacturer's protocol.

RNAiFect 是一种基于脂质的转染试剂，专门用于 siRNA 转染，确保没有 RNAse 活性。

For a big chunk of credit-card losses; the number of filings (and thus charge-off rates) would be rising again, whether

年美国个人破产法的一个改动使得破产登记急速下降，而后引起了信用卡大规模的亏损。

Eph. 4:23 And that you be renewed in the spirit of your mind

弗四23 而在你们心思的灵里得以更新