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Primers used in PCR were designed based on the sequence of mitochondrial 16S rDNA (AJ250642 and AF312718) and beta-actin (AY910691) of Eriocheir sinensis which had been deposited in GenBank. PCR analysis using total DNA from muscle, branchia, testis and sperm indicated that 16S rDNA exhibited different expression in the four samples. Beta-actin gene was detected plentiful in all the four tissues/cells and exhibited the same expression pattern. 16S rDNA gene amplification was detected at high levels in muscle and branchia, appreciable low in testis and very low in sperm. The PCR products of 16S rDNA gene were sequenced and aligned with the sequence of 16S rDNA from GenBank (AJ250642 and AF3 12718), and the alignment result showed there was no difference between them.

通过GenBank中的中华绒鳌蟹线粒体16S rDNA序列设计引物,利用PCR扩增方法,以beta-actin做内参,检测了中华绒螯蟹肌肉、鳃、精巢和精子中线粒体16S rDNA扩增情况,发现各组织或细胞beta-actin扩增片段大小、条带宽度和亮度基本一致,表明各模板DNA量基本一致;而在同一DNA浓度下,16S rDNA的扩增片段长度虽一致,但其产物量存在明显差异,精子16s rDNA产物量显著低于其他3种组织,其条带宽度和亮度很弱;16S rDNA扩增产物经测序分析及比对证明与已有序列完全吻合。

This passeage discusses the sequence of cauchy criterion function limit, the convergence of cauchy criterion, the convergence of the series, the function of cauchy criterion listed uniform convergence of cauchy criterion function series, uniform convergence of cauchy criterion, plane of cauchy criterion, some abnormal integral parameter uniform convergence of cauchy criterion and summarized and proof, and through a lot of sample reflected their status and role.

马上要交论文了,摘要不晓得如何翻译,现在请诸位高人帮助翻译1下,不能在线翻译哦,高悬赏~~~~~~感谢本文主要论述了数列地柯西收敛准则,函数极限存在的柯西准则,级数收敛的柯西准则,函数列一致收敛的柯西准则,函数项级数一致收敛的柯西准则,平面点列的柯西准则,含参量非正常积分一致收敛的柯西准则的应用并进行了总结和证明,并通过大量的例题体现了它们的地位和作用。

The vapour and plasma ignition times of the target are got based on the equation of heat conduction and cascade model. We suppose that the plasma is ignited when 210 generations of new electrons are born in vapour generation time. The influence of temperature on the thermodynamics and optics parameters of material also have been considered in the model. The resulting theory has good agreement with the experiment and overseas report. A blade method to measure the laser spot is given in this paper with validation and error analysis. The plasma threshold of metals in atmosphere and water ambients both are diagnosed with the light deflection and piezoelectric transducer. A Q-switched pulsed Nd:YAG laser operating at infrared (1064nm), visible(532nm) and ultraviolet (355nm) wavelengths has been used. Al、Fe、Cu are used as targets and get a similar results with both method. Theoretical and experimental analyses are applied on the influence of wavelength on the threshold have been done, both of which have shown that the plasma threshold of metals decrease as the laser wavelength increases; The plasma threshold of metals are higher in water than in air and the pressure of the shock wave in water is five times higher than in air.

从热传导方程和雪崩电离机制出发,假设当电子增值2~(10)倍时,考虑了温度对材料热力学和光学参数的影响时,得到了气化和等离子体点燃的时间,利用该模型进行计算得到的结果与国内外报道及自行通过实验测得的阈值基本一致;提出了利用刀刃法测量激光光斑面积的方法,并通过实验进行了验证和误差分析;利用光偏转装置和压电换能器分别对空气和水中金属等离子体点燃阈值进行了实验诊断,激光器均为调Q—YAG激光器(波长1064nm,532nm,355nm,脉宽10ns),靶材分别为Al、Fe和Cu,两种测试方法得到的等离子体点燃阈值基本一致;本文从实验和理论计算两个方面讨论了波长对等离子体点燃阈值的影响,均得到了等离子体点燃阈值随着波长的增加而减小的结论;对空气中和水中不同环境下金属等离子体的点燃阈值进行了比较研究,得到了金属在水中的等离子体点燃阈值比空气中的大,且水中产生的冲击波的压强是空气中的5倍左右的结论。

In this paper a decision method of uniform convergence for function sequence is given, and a few of properties for the uniformly convergent function-sequence are obtained by this method, which is used in the construction of a new uniformly convergent function-sequence by others known.

给出了一个定理用于判定函数列的一致收敛,通过此判定定理,又得出了由一致收敛函数列构造新的一致收敛函数列的几个性质。

It has the following characteristics: 2D drawings can be maintained with a high degree of consistency of 3D model; CAD /CAPP /CAM are integrated by the generation of comp lete manufacturing information and the integrated digital p roduction line of shell, outfitting and painting is imp lemented basically; the generation of comp lete manufacturing and management information of shell, outfitting and painting lays the foundation for the integration of design, manufacturing and management; the entity and parametrized technology is used to ensure the establishment of ship p roductmodelwith comp lete topology relations; and the data interfaces for the heterogeneous CAD data and information integration ensure the collaborative design for the factories and the institutes.

SPD系统具有如下特点:能保持三维模型与二维图纸的高度一致;通过生成完整的加工制造信息实现CAD/CAPP/CAM集成,基本打通&壳、舾、涂一体化&的数字化生产线;通过生成壳、舾、涂完整的制造和管理信息,为实现船舶&设计、制造、管理一体化&奠定基础;通过实体和参数化技术为建立具有完整拓扑关系的船舶产品模型提供有力手段;并通过与其他系统的数据接口实现异构CAD的数据和信息集成,为厂、所协同设计创造技术条件等。

The 352 valid samples were analyzed by SPSS. The results showed there was no distinct corresponding relationship between the product attributes and reaction time. The different key attributes from questionnaire importance rating and the shortest reaction time standards were used to regressively analyze the results of customers' overall rating (such as overall satisfaction,objective quality, recommend intention).The results indicated that the coefficiency of regression of the special attributes chosen from reaction time to overall rating was distinct, while the coefficiency of the special attributes chosen from importance rating to overall rating was not. The main conclusions are: 1. Regarded attributes can be obtained by the reaction time of brand performance rating.

本实验的有效样本为352个,通过SPSS软件进行分析统计,数据统计结果显示,在品牌表现评价反应时与其重要性问卷评定结果的相关系数的检验中,并未发现品牌表现评价反应时与品牌属性重要性评定结果之间的显著相关;将由问卷重要性评价最高标准所得到的关键属性与通过品牌表现评价反应时最短的标准所选取的关键属性的品牌表现分别对消费者的总体评价结果(如&总体满意度&、&主观质量&、&推荐意向&)进行回归分析,发现通过品牌表现评价反应时方法选取的特殊关键属性(与问卷重要性评定法选取不一致的关键属性)的品牌表现对总体评价结果的回归系数显著,而重要性评定问卷法选取的特殊关键属性(与品牌表现评价反应时法选取的不一致的关键属性)的品牌表现对总体评价结果回归系数不显著。

Ostwald ripening is a key mechanism in the destabilization of emulsions (for example, by creaming and sedimentation).

同时最近几年,Banfiled又提出了一种新的晶体生长机制也能形成单晶结构,oriented attachment,多个取向不一致的单晶纳米颗,通过粒子的旋转,使得晶格取向一致,向后通过定向附着生长使这些小单晶生长成为一个大单晶,当然定向附着的过程出难免会出现一些位错和缺陷,这种生长机理形成的单晶的特点同Ostwald ripening不同,OR形成的单晶大多是规则的,给材料本身晶体结构相关,而OA形成的单晶结构在形貌上则没有限制,任何形状和结构的单晶材料都能通过此机理形成。

In the early research, HSV color space is considered to be uniform with human's vision model. Along with the analysis of HSV space, we find the uniform is limited and propose a new quantization method, which is more reasonable to human's vision model. Without decreasing the retrieval performance, the scheme non-uniformly quantizes the color space into 36 bins. Because the quantization result has only 36 bins, the computational complexity is decreased effectively while the performance increases. Relevance feedback is an interactive technique in the process of CBIR.

在以前的研究中,一直认为HSV色彩空间与人的视觉感知是一致的,本文通过对HSV色彩空间非等间隔量化的研究,发现这种一致是有限的,通过对HSV色彩空间的分析,本文对相似色区域的划分提出了从视觉上更合理的不等间隔的量化方法,使其更符合人的主观视觉模型,并且在不降低检索性能的前提下,使色彩量化为36种颜色,有效地降低了算法复杂度,提高了检索效率。

The plasma ignition threshold of metal is got through experimental and numerical study. The vapour and plasma ignition times of the target are got based on the equation of heat conduction and cascade model. We suppose that the plasma is ignited when generations of new electrons are born in vapour generation time. The influence of temperature on the thermodynamics and optics parameters of material also have been considered in the model. The resulting theory has good agreement with the experiment and overseas report. A blade method to measure the laser spot is given in this paper with validation and error analysis. The plasma threshold of metals in atmosphere and water ambients both are diagnosed with the light deflection and piezoelectric transducer. A Q-switched pulsed Nd:YAG laser operating at infrared (1064nm), visible(532nm) and ultraviolet (355nm) wavelengths has been used. Al、Fe、Cu are used as targets and get a similar results with both method. Theoretical and experimental analyses are applied on the influence of wavelength on the threshold have been done, both of which have shown that the plasma threshold of metals decrease as the laser wavelength increases; The plasma threshold of metals are higher in water than in air and the pressure of the shock wave in water is five times higher than in air .

从热传导方程和雪崩电离机制出发,假设当电子增值210倍时,考虑了温度对材料热力学和光学参数的影响时,得到了气化和等离子体点燃的时间,利用该模型进行计算得到的结果与国内外报道及自行通过实验测得的阈值基本一致;提出了利用刀刃法测量激光光斑面积的方法,并通过实验进行了验证和误差分析;利用光偏转装置和压电换能器分别对空气和水中金属等离子体点燃阈值进行了实验诊断,激光器均为调Q-YAG激光器(波长1064nm,532nm,355nm,脉宽10ns),靶材分别为Al、Fe和Cu,两种测试方法得到的等离子体点燃阈值基本一致;本文从实验和理论计算两个方面讨论了波长对等离子体点燃阈值的影响,均得到了等离子体点燃阈值随著波长的增加而减小的结论;对空气中和水中不同环境下金属等离子体的点燃阈值进行了比较研究,得到了金属在水中的等离子体点燃阈值比空气中的大,且水中产生的冲击波的压强是空气中的5倍左右的结论。

The method to configure RED parameters based on network properties is also presented. Simulations show that the modified Random Early Detection algorithm really performs better than the original Random Early Detection algorithm and can work for a much wider range of traffic. Finally, whether the assured service mechanisms i. e. an edge router tags every arriving packet of a TCP flow based service profile and core router drops packets differently at congestion using active queue management scheme is possible to provide a throughput consistent with target rate, is investigated under different network scenarios. Different factors including target rate tagged algorithm, RIO algorithm and TCP congestion control mechanism how to affect the throughput of an assured service TCP flow are examined. Based on a fluid model. we then derive a model of an assured service TCP flow, which is the function of round trip delay, packet loss rate and token bucket parameters. Based on this model, we observe that in some cases it is possible to regulate the token bucket parameters to gain a consistent throughput with the target rate, while in other cases there exist ranges of values of the achieved rate for which the token parameters have no influence. With the latter, some modified TCP congestion control mechanisms to assure TCP throughput are proposed.

最后,在区别服务网络结构内,研究了当前提出的一种边缘路由器根据服务合同标记、分类数据包,结合内部路由器采用主动队列缓冲管理机制区别处理数据包的确信服务机制是否可在不同网络情况下使TCP连接获得同目标速率相一致的、公平的吞吐量问题;指出影响TCP连接获得同目标速率相一致吞吐量的主要因素是目标速率大小、标记算法参数和TCP连接的拥塞控制算法;对此,基于流模型假设,首次推导出一个以端到端时延、数据包丢失率、漏桶参数为变量的确信服务TCP连接吞吐量模型;利用这个模型进行分析,得到主要结论是在一些情况下可通过设置合适的漏桶参数得到同目标速率更一致的吞吐量,而在另一些网络情况下不管如何设置漏桶参数都不能使TCP连接获得同目标速率一致的吞叶量;对于后一些情况,还初步讨论了改进TCP拥塞控制算法来改善TCP连接性能的方法。

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推荐网络例句

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。

There is no differences of cell proliferation vitality between labeled and unlabeled NSCs.

双标记神经干细胞的增殖、分化活力与未标记神经干细胞相比无改变。