一致通过

Mycorrhizal spores in most rhizospheric soil of weed species, except Vida cracca, TrfoHum npens and Oxalis cornculata, decreased significantly. 2 Influence of coexisting of various plant species on AMF colonization in Pb polluted soil Comparing with monoculture,the results showed mat diversity did not affect on die AMF colonization of weed plants planted in soil at the concentration (334.5251mg/kgsoil or nonpolluted soilsignificandy.In the experiment of species combination with Trfolium repens, Lolium perenne, Poa annua, Plan tago virginica, the infection rate of all species increased in Pb treatment except Poa annua, implying that diversified plant species coexistence may alleviate the impacts of Pb on mycorrhizal colonization.

铅污染土壤条件下不同植物物种互作对菌根的影响通过盆栽加铅模拟试验，在铅污染(334.5251mg／kg土)的土壤中，黑麦草、早熟禾、白车轴草和北美车前无论是对照还是铅处理，多植物物种共存组合中的杂草菌根侵染率与单个物种相差不大，AMF菌根侵染率的处理间差异的变化趋势在铅污染的土壤和对照土壤条件下表现一致，但铅处理与对照相比，除了早熟禾外，菌根侵染率均升高，说明杂草多样性对菌根有一定的保护作用。

In this experiment, we screen the major protective antigen gene-SOD gene of M. paratuberculosisin order to study the sensitive, specific diagnostic reagent and prophylaxis preparation, especially theDNA vaccine. The SOD gene was amplified from Mycobacterium paratuberculosis C-2 chromosomalDNA by using the PCR technique and cloned into pMD18-T Vector System. We gained a SOD gene of624bp.The recombinant clone was identified byα-complementarity, enzyme digestion and PCRidentification. The result indicated that the recombinant plasmid pMD18-T-SOD was successfullyconstructed. Moreover, through sequential determination and DNASTAR analysis between the clonedSOD gene of M. paratuberculosis C-2 and that of the M.paratuberculosis K-10 strain, the sequentialhomogeneity reached 99%, and the amino acid homogeneity reached 99.5%. The preceding analysisindicated that the SOD gene was very conservative in M. paratuberculosis.

为了研制副结核病敏感、特异的诊断试剂和新型、高效的预防制剂，尤其是DNA疫苗，本研究筛选了M.paratuberculosis主要保护性抗原SOD基因，以M.paratuberculosis C-2染色体DNA为模板，以SOD基因的特异性引物进行PCR扩增，获得了624bp的SOD基因，通过T-A克隆技术，将PCR产物克隆至pMD18-T Vector中，以质粒大小、酶切分析、PCR扩增及序列分析鉴定重组克隆，成功地构建出克隆质粒pMD18-T-SOD，序列测定及DNASTAR分析表明，所获得的M.paratuberculosis C-2 SOD基因与Gen Bank中M.paratuberculosis K-10 SOD基因的大小完全一致，两者核苷酸序列的同源性为99％，氨基酸序列的同源性为99.5％，表明该基因在副结核分枝杆菌中是高度保守的。

It also can be used as alternative antigen of newgeneration vaccine.In this experiment,we screen the major protective antigen hsp65 gene of MAP in order to developnew vaccine especially the DNA vaccine for the prevention of paratuberculosis disease.The hsp65 genewas amplified from MAP C-2 chromosomal DNA by using the PCR technique.We gained a hsp65 gene of 1 626bp.Then PCR product was cloned into pGEM-T vector by T-A clone technique and therecombinant clone was identified by plasmid size,enzyme digestion and PCR identification.The cloneplasmid of pGEM-T- hsp65 was successfully constructed.The nucleotide sequence and deduced aminoacid sequence ofclone gene was analyzed by DNASTAR software.The result indicated that the size ofhsp65 gene consist with M.paratuberculosis K-10 strain in GenBank and the sequential homogeneityreached 99.1%,the amino acid homogeneity reached 99.3%.The preceding analysis indicated that thehsp65 gene was very conservative in M.paratuberculosis.

为了研发预防副结核病的新型疫苗尤其是DNA疫苗及相关蛋白功能，本研究选择了MAP的主要保护性抗原Hsp65蛋白，以副结核分枝杆菌C-2株染色体DNA为模板，以hsp65基因的特异性引物进行PCR扩增，获得了1 626bp的hsp65基因，通过T-A克隆技术，将PCR产物克隆至pGEM-T Vector中，以质粒大小、酶切分析、PCR扩增及序列分析鉴定重组克隆，成功地构建出克隆质粒pGEM-T-hsp65，以DNASTAR软件分析了所克隆基因的核苷酸序列和推导的氨基酸序列，结果表明，所获得的hsp65基因与GenBank中MAPK-10株该基因核苷酸大小完全一致，两者核苷酸序列的同源性为99.1％，氨基酸序列的同源性为99.3％，表明该基因在副结核分枝杆菌中高度保守。

Five development planning, the same medicine uphold the overall strategic thinking, based in Shenzhen, south China focus, unswervingly follow the research and development, pharmaceutical, distribution, logistics, retail integration path of development through a comprehensive The implementation of intensive, information-based and scale of operations, as soon as possible to become Guoyao holding in the Southern District of the Chinese pharmaceutical distribution, logistics, production, research and development and investment centre, build China's first Southern Chinese medicine brands.

五发展规划中，一致药业秉承全局战略思路，立足深圳、着眼华南，坚定不移地走研发、制药、分销、物流、零售一体化发展道路，通过全面实施集约化、信息化和规模化经营，尽快成为国药控股在中国南区的医药分销、物流、生产、研发和投资中心，打造中国南区第一医药品牌。

In normal years, the onset time of summer monsoon over the SCS is approximately in the fourth pentad of May.

通过对各种指标确定夏季风爆发时间一致年份的大气环流特征分析表明，延伸到南海北岸附近地区的海上锋区对流加热和孟加拉湾北部陆地上的对流加热、沿东亚近海向西太平洋推进的冷空气是控制南海夏季风爆发的主要因素。

With this doublecheck, the percentage of error did not exceed 0.5 percent, and there was not a single report of incompatibility during the course of the war.

通过这种重复实验，误差不超过0.5%，并且战争期间没有哪份报告是不一致的。

The synthesis scheme we developed can deal with changes in visibility, produce perspectively correct views and handle the distortion around the circumferences in the synthesized views. In our scheme, the area without corresponding points was first determined by using Fourier transformation in order to deal with distortion problem, within the mutual overlapped area the z-buffer values of matched key points were computed so that the visibility in novel views can be handle correctly, lastly the common points in new views are rendered through epipolar geometry constraint.

算法的步骤为：利用傅立叶变换方法确定相邻源图像无对应点的区域；然后，利用尺度空间和相位一致原理在共同区域提取及匹配边缘上的关键点，计算其深度值，并在新图像中由该值判别虚拟图像中的可见性；再由关键点恢复极线约束，通过极线间的位置和颜色插值生成虚拟图像中有对应区域的一般点；在确定极线位于无对应点区域段后，利用加权平均或放大插值的方法实现了源图像无对应点区域在虚拟图像中的自然过渡。

Stopped running in the system, the entire network piezometer head has reached the same trend, while reducing the differential pressure control valve through the opening best to maintain the original pressure has remained basically unchanged until the differential pressure control valve closed.

在系统停止运行时，整个网路的测压管水头有达到一致的趋势，而压差控制阀则通过减小开度竭力维持原有的压差基本不变，直至压差控制阀关闭。

The velocity and planarity of the shock wave were measured. The radiation temperature measured by soft X-ray spectrometer was consistent with the result by optical streak camera.

通过冲击波法测得的腔内辐射温度与软X光能谱仪的测量结果一致，验证了冲击波速度测量的可信度。

Based on the principle of PEC, surface cracks mainly affect the high frequency components and subsurface crack mainly affect the low frequency components, so the PEC signals can be analyzed in frequency domain, which can eliminate the influence of noises during measurement. A method of frequency spectrum separating point is proposed for flaw classification, which enhances the accuracy in classification of defects. The agreement between theory and experiment shows that the present method is valid and applicable.

通过对脉冲涡流的理论分析发现，表面裂纹主要影响脉冲频谱中的高频成分，而表面下裂纹主要影响脉冲频谱中的低频成分，因此可以将时域中易受噪声影响的脉冲涡流信号变换到频域分析，提出了"频谱分离点"的方法来对裂纹进行分类识别，提高了缺陷分类识别的正确率，理论分析和实验结果相一致，证明了该方法的正确性和有效性。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。