一种杆菌的

in order to establish a rapid, sensitive and specific detection method for identification of four kinds of food-borne pathogenic bacteria including salmonellla spp, listeria monocytogenes, staphylococcus aureus and bacillus cereus by using multiplex pcr, according to invasion protein a geneof salmonellla spp, transcriptional regulatory protein gene of listeria monocytogenes, autolysin gene ofstaphylococcus aureus and gyrase b subunit gene of bacillus cereus, four pairs of specific primers were designed for multiplex pcr amplification.

小 摘要：为建立一种利用多重pcr技术检测和鉴定沙门氏菌、单核细胞增生李斯特菌、金黄色葡萄球菌和蜡样芽孢杆菌的方法，根据沙门氏菌侵袭力蛋白a基因、单核细胞增生李斯特菌蛋白转录调控基因、金黄色葡萄球菌自溶素基因、蜡样芽孢杆菌的促旋酶b亚单位基因设计引物，进行多重pcr扩增，并对多重pcr反应体系进行优化。

The invention discloses a preparing method of bacillus alcaligenes strain Alcaligenes sp., CCTCC No.M206121 of degraded o-nitrophenol, which comprises the following steps: allocating inorganic salt culture medium with dibasic sodium phosphate, monobasic potassium phosphate, manganese sulfate, copperas, addex-magnesium, calcii chloridum, blue copperas, white copperas and hydrogen dioxide solution; adjusting pH value; sterilizing under high temperature; enriching bacterial; sampling from sludge; allocating suspending liquid with soil sample and inorganic salt; seeding into ortho-nitrophenol fresh organic salt culture medium with finite volume seeding quantity; keeping the temperature; culturing on shaker; getting enriched culture; separating and purifying enriched culture in LB solid culture medium.

本发明公开了一种降解邻硝基酚的产碱杆菌菌株及制备方法，产碱杆菌为Alcaligenes sp。，CCTCC No.M206121。其制备步骤是：首先是配制无机盐培养基，成分为磷酸氢二钠、磷酸二氢钾、硫酸锰、硫酸亚铁、硫酸镁、氯化钙、硫酸铜、硫酸锌和双蒸水，调pH值高压灭菌；其次是菌种的富集，从污泥中取样，将土样与无机盐培养基配制成悬浮液，将悬浮液以一定体积接种量接种到含邻硝基酚的新鲜无机盐培养基中，恒温，摇床培养，获富集培养物；第三是将富集培养物在LB固体培养基中分离和纯化。

Proteus is a gram-negative, rod-shaped bacteria of the genus Proteus, certain species of which are associated with human enteritis and urinary tract infections.

变形杆菌，一种革兰式阴性的杆状细菌，某些变形杆菌与人类的肠道和尿道感染有关。

The Studies on Growth and Sporulation Characteristics of Bacillus popilliae DING Ying, WANG Ruo-han, WANG Jin-feng (School of Pharmaceutical Science and Technology, Tianjin University, Tianjin 300072, China) Abstract: The spores of Bacillus popilliae which are specialized pathogens of the scarabaeid beetles are difficult to be reproduced in vitro.

日本金龟子芽孢杆菌生长和孢子生成阶段的特性研究丁莹，王若菡，王劲峰(天津大学药物科学与技术学院，天津 300072)摘要：日本金龟子芽孢杆菌是一种在活有机体外很难产生孢子的昆虫病原体。

The aim of this research is to find out the experimental parameters that affecting the results when analyzing bacteria by MALDI TOF MS, and to set up a general sample preparation protocol for establishing mass database of Bacillus anthracis and Yersinia pestis. It will play an important role in rapid identification of bacteria, and tracing its origin in the scenario of bioterrorism and outbreak of infectious disease for microbial forensic analysis. Methods:As for the universalness of sample preparation protocol for bacterial analysis by MALDI TOF MS, bacteria being investigated included: gram-positive bacteria including B.

本研究通过对MALDI TOF MS分析细菌的方法进行系统的研究，阐明影响MALDI分析的重要实验因素的作用，并建立MALDI TOF MS分析细菌的标准实验方法，从而实现不同菌种和同一种内不同菌株的鉴定，为建立炭疽芽孢杆菌和鼠疫耶尔森氏菌的质量图谱数据库奠定基础，从而能够快速准确地检测和鉴定我国不同来源的炭疽芽孢杆菌和鼠疫耶尔森氏菌，在生物恐怖或传染性疾病爆发时，作为微生物法医学的一种重要技术，快速、准确地鉴定细菌，并追踪其来源。

The production of Feeding Bacillus Microbiological- Candy Peptide Element is a kind of additive with high-quality , high-efficient , and without public nuisance. It is consist of some active substance as feeding bacillus and so on, and it is refined by the technology of contemporary cell, gene and the regulation of zymolysis.

项目简介：饲用芽孢杆菌微生态复合制剂-"糖肽素"产品是一种优质高效无公害添加剂预混合饲料，是由饲用芽胞杆菌等活性物质组成的微生态复合制剂，经现代细胞和基因工程及代谢调控发酵技术精制而成。

These results indicate that PCR assay is a rapid, specific and sensitive detection method for Proteus mirabilis, and it is suitable for daily monitoring and rapid diagnosis of Proteus mirabilis.

建立了一种快速、准确检测奇异变形杆菌的PCR方法，适合奇异变形杆菌日常监测和快速诊断的需要。

In the generalization of contact lens develops day by day, on the issue associated with corneal infection due to incorrect habit of contact lens is put much emphasis. Pseudomonas aeruginosa is one of the most important infectious pathogens, it will adhere on the cornea and destroy the corneal tissue opportunistically with its quite violent virulence. The infected corneas will be attacked to necrosis by one to two days with very fast destructive speed. It is very important to diagnosis the infectious pathogen in time.

在隐形眼镜愈来愈普及的趋势下，因隐形眼镜配戴习惯不正确造成角膜感染的议题也逐渐受到重视，而绿脓杆菌正是其中最重要的一种感染病菌，其会附著在角膜上，然后伺机地破坏角膜组织，绿脓杆菌毒性相当猛烈，故其破坏的效率相当地快，角膜大概一至二天就会被摧残溃烂，因此即时诊断出角膜感染的病原体是相当重要的。

A polypeptide antibiotic, produced by the soil microorganism Bacillus brevis, that is a major constituent of tyrothricin.

短杆菌酪素一种多肽抗生素，由土壤中的微生物土壤杆菌产生，是混合短杆肽菌的重要组成部分

A polypeptide antibiotic,produced by the soil microorganism Bacillus brevis,that is a major constituent of tyrothricin.

短杆菌酪素一种多肽抗生素，由土壤中的微生物土壤杆菌产生，是混合短杆肽菌的重要组成部分收藏反馈

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。