一盒

One was the pqsA' positive expression plasmid constructed by cloning the pqsA'-lacZ fusion digested from pYHP441 into miniCTX-1, whose sequence was then integrated into wild type PAO-1 chromosome by biparental mating procedure. The other was pqsA-E operon knock-out plasmid whose sequence between pqsD and pqsE operon was inserted by tetracycline cassette by the site specific insertion mutagenesis strategy and the mutant constructed by biparental mating of S17-1 that harbored the plasmid and pqsA' positive expression mutant.

一种是酶切质粒pYHP441获得pqsA'-lacZ片段后，亚克隆入质粒miniCTX-1中，构建成PqsA'的阳性表达质粒，随后将构建的质粒，通过双亲交配过程整合入野生型铜绿假单胞菌株PAO-1染色体组中；另一种是通过点特异插入诱变策略，将四环素基因盒插入启动子pqsD和pqsE之间，构建的阴性质粒转化入大肠杆菌S17-1株后，和上述pqsA'阳性表达突变株进行双亲交配过程。

Now our workshop area is about 100, 000 square meters and our main production equipment is two sets of German MAN Roland R904-7B type outsize four color offset press which is the biggest paper range(1.6m*1.2m) of offset printing machine in the world, one set of Heidelberg speed master four color sheet-fed offset press, one set of Roybi four color offset press, two sets of automatic folder gluer, two sets of film blowing machines, one set of UV machine and nine sets of die-cutting and creasing machines.

现在我们的车间面积约10万平方米，主要生产设备有两套德国男子罗兰r904-106型outsize四色胶印机，是最大的纸张范围(1.6m*1.2m)胶印机在世界上一部海德堡速霸四色单张纸胶印机，一套roybi四色胶印机，两套糊盒机，两套吹塑机，一台机器紫外线和9套模切和压纹机器。

What I want to tell you is that this event led to more than ten deaths of citizens and one death of police in Lasa, five young girls were burnt to death alive on the second floor of a rent house!

我想告诉你们的是这次事件导致十多名西藏市民的死亡盒一名武警的死亡，5名花季少女在一件出租屋的二楼被活活烧死！

What s even creepier is that it houses two displays insides its glass tummy that animates ever so often changing between being a chronometer, magnifying glass, mirror, compass, news box, phone, media player, and TV which frankly looks a little funny considering all the action takes place with a mysterious dark background that tends to steal all the focus away from the screen itself!

更加神奇的是，它的两个显示屏都隐藏在玻璃表面之下。那些玻璃经常能发挥不同的功能，比如计时计、放大镜、镜子、指南针、消息盒、手机、媒体播放器、以及一个电视。一想到所有的情节都在一个神奇的黑色背景下播放，而这个背景本身又将通过其屏幕，吸引所有的注意力，就觉得这看起来是一件十分好笑的事情。

At noon yesterday she traipsed out of the Carrefour shopping mall clutching bags chockfull of the little things that she adores: a pink chiffon skirt, a canister of salty barbecue-flavored potato chips, three boxes of mouth-watering Japanese cookies, and several cans of tangy purple grape juice.

昨日午时，她自家乐福商场出来，怀中搂着一个大纸袋，里面装着一条粉色的雪纺纱连衣裙，一筒烧烤味的薯片，三盒日本味的饼干，及几罐紫色的葡萄汁。

Ling 100 is a bird of the background skin care brand, has been very far away from us, and only an old hand, you can once again揭去silver tin box that layer of platinum, with the index finger of a small point of white dipping凝脂, then抹匀palm, yellow painted all over our memory - It is that in those days of make-up the most classic way to make the kind of lingering flavor氤氲kept in our hearts, and let us yellow memory always maintained adequate moisture, will never be a result of a long era chapped and dry.

百雀羚是一个护肤品牌的背影，离我们已经很远了，惟有一只怀旧的手，还可以再一次揭去盒内那层银色锡铂，用食指蘸上一小点乳白色凝脂，然后在掌心抹匀，涂遍我们发黄的记忆——正是这种在那个年代最经典的化妆手法，才使那种挥之不去的氤氲气息一直保留在我们心底，并让我们发黄的记忆始终保持着足够的水分，永远不因年代的久远而干燥皲裂。

The CD box holder comprises a substantially flat base plate.

一种CD盒保持架包括一基本平坦的基板。

Brush grip structure pattern: It is 2 20 ° tilt of the brush box symmetry with a crossbar to connect into a whole pairs of inclined constant pressure brush holder.

刷握的结构型式：是两只20°倾斜的刷盒对称用一横梁连接成一整体的双斜式恒压刷握。

Generally speaking, as long as you are a good measure of the total width of the window and the job can calculate the required amount of cloth, but if the measure on its own, then have to pay attention to some details, such as the window of a high degree of measurement from the start Chuang Lianhe To outweigh the lower edge of the window will not be emptied of its width is also probably have to pay more on the side of 5-10 cm, but if they are Banyao have to pay attention to whether there is a window sills, to be done within the window frame, then have to carefully Measure may not yield more than half a centimeter card does not go in and a half centimeters or less emptied of the situation, the track can be installed on the wall or ceiling should also note that some tracks can be linked to the ceiling can also be linked to the walls, some not I suggest you bring home a good-size windows notes, the regional states, take good pictures of each window to the stores, they will suggest you how to use the track and practice

一般来说只要您丈量好窗户的总宽和总高就可以计算所需的布量，但如果自行丈量的话有些细节得注意，比如说窗的高度得从窗帘盒内开始丈量到盖过窗的下缘才不会走光，宽度也是，大概一边都得多加个5-10公分，但如果是半腰窗就得注意是否有窗台，要做在窗框内的话就得仔细丈量不然有可能产多了半公分卡不进去或是少了半公分走光的状况，轨道可以安装的墙面或是天花板也要注意，有些轨道是可以挂天花板也可以挂墙壁的，有些不行，建议你把家里的窗户尺寸做好一份笔记，写明区域，拍好每一窗的照片给店家看，他们会建议您用怎样的轨道和做法

Some users used to direct hand box back plate, which drives the drive gear is a kind of damage, it is proposed to overcome this bad habit of users.

有些用户习惯用手直接推回盘盒，这对光驱的传动齿轮是一种损害，建议用户克服这一不良习惯。

Singer Leona Lewis and former Led Zeppelin guitarist Jimmy Page emerged as the bus transformed into a grass-covered carnival float, and the pair combined for a rendition of "Whole Lotta Love".

歌手leona刘易斯和前率领的飞艇的吉他手吉米页出现巴士转化为基层所涵盖的嘉年华花车，和一双合并为一移交&整个lotta爱&。

This is Kate, and that's Erin.

这是凯特，那个是爱朗。