一滴

METHODS: Bone of limbs were obtained under sterile condition, washed by PBS, centrifugalization, and dropwise into centrifuge tube containing 1.073 g/mL Percoll separating medium, centrifugalization, and then adding L-DMEM containing 10% fetal bovine serum, benzylpenicillin sodium and phytomycin, cultured in a incubator at 37 ℃ with 5% saturated humidity. The culture medium was renewed after 48 hours, and non-adhesive cells were removed.

无菌条件下截取流产胎儿双侧四肢骨，PBS冲洗髓腔，离心去脂肪及上清液，L-DMEM重悬细胞，沿管壁缓慢滴加至底部有等量1.073 g/mL Percoll分离液的离心管中，离心后吸取中间界面白膜层，加入含体积分数为10%的胎牛血清、青霉素钠、链霉素的L-DMEM完全培养基，接种后置于37 ℃、体积分数为5%的CO2饱和湿度孵箱内培养。48 h后换液，去除未贴壁细胞，以后每2 d换液一次。

The puerperant and her relatives all signed informed consents. METHODS: Bone of limbs were obtained under sterile condition, washed by PBS, centrifugalization, and dropwise into centrifuge tube containing 1.073 g/mL Percoll separating medium, centrifugalization, and then adding L-DMEM containing 10% fetal bovine serum, benzylpenicillin sodium and phytomycin, cultured in a incubator at 37 ℃ with 5% saturated humidity.

无菌条件下截取流产胎儿双侧四肢骨，PBS冲洗髓腔，离心去脂肪及上清液，L-DMEM重悬细胞，沿管壁缓慢滴加至底部有等量1.073 g/mL Percoll分离液的离心管中，离心后吸取中间界面白膜层，加入含体积分数为10%的胎牛血清、青霉素钠、链霉素的L-DMEM完全培养基，接种后置于37 ℃、体积分数为5%的CO2饱和湿度孵箱内培养。48 h后换液，去除未贴壁细胞，以后每2 d换液一次。

The Antiviral substance was induced from blastulae embryonic cell line of crucian carp by ultra violet inactivated grass carp reovirus.

用紫外线灭活的草鱼呼肠孤病毒诱导鲫鱼囊胚细胞能产生一种高滴度的抗病毒物质。

We observed changes of GV chromatin configurations in porcine, caprine and bovine oocytes from follicles of different sizes and degrees of atresia and during maturation in vivo and in vitro, using a new method for preparation of GV samples.

本项目发明了一种特殊的卵母细胞生发泡标本制备技术，能克服脂肪滴等对生发泡染色质构型观察的阻碍；经Hoechst或CMA3染色后可清楚地观察染色质构型的变化。

So the conductometric titration method can be used for measuring the composition of water-soluble copolymer containing ion monomer through complex reaction between polyelectrolyte and surfactant.

利用电导率的这一转折性变化，建立起了复合作用电导滴定法测定共聚物P(AM- co -NaAMPS)分子链中磺酸盐单体NaAMPS含量的新方法。

In order to simulate the liquid fuel atomization in supersonic crossflow, a hybrid spray atomization model was developed.

为了模拟超声速气流中液体射流的雾化，发展了一种液滴破碎混合模型。

To simulate the steady removal VOC waste gas degradation parallel plat plates theoretical model, the result shows that the toluene removal efficiency curse can track the dentiform and sine wave reference curves and five-step predictive control can track reference curve closely compared with one step predictive control.

并对稳态的生物膜滴滤床降解甲苯净化效率的平行平板通道理论模型进行了多步预测控制的仿真研究，得到结果是，基于神经网络的预测控制能使得甲苯降解效率跟踪参考的齿形轨迹和正弦轨迹，五步预测控制较一步预测控制更能较好的逼近跟踪轨迹。

Originally techniques dropped formaldehyde into the mixture of phosphorous acid and monosodium of imino diacetic acid in high temperature.

原工艺采用的是在酸性条件下高温时将甲醛滴加到亚磷酸与亚氨基二乙酸一钠盐混合溶液中进行反应，考虑到工业上亚磷酸的合成一般采用三氯化磷水解、提浓、结晶制得，直接将三氯化磷用于双甘膦的合成，不仅可以简化操作过程，而且对于降低生产成本和能耗有明显意义。

The components of the hydrochloric acid and the fire_retardant - an organic diacid in the waste water produced by production of the fire_retardant were analysed rapidly by acid base potentiometer.

采用酸碱电位滴定法快速简便地测定了阻燃剂生产中产生的含有盐酸和阻燃剂———一种有机二元酸的含量。

This paper is studied on a new synthesis method of amino-modified organic silicone micro-emulsion of cationic style by one step with a silane-alkyl coupling,3-(2-aminoethyl)-aminopropyl methyl dimethoxy silane while opening-cyclo polymerization of the octa-methyl cyclo tetra siloxane,thus the optimal manufacturing conditions are determined by comparing test of their properties.

采用硅烷偶联剂氨乙基氨丙基甲基二甲氧基硅烷改性，八甲基环四硅氧烷开环聚合，探索一步合成阳离子型氨基改性有机硅微乳新方法，通过其性能测试得出其最佳工艺条件：反应温度：80℃，反应时间：3 h，十六烷基三甲基溴化铵用量：5%，脂肪醇聚氧乙烯醚用量：5%，预乳液滴加时间：2 h。

With Death guitarist Schuldiner adopting vocal duties, the band made a major impact on the scene.

随着死亡的吉他手Schuldiner接受主唱的职务，乐队在现实中树立了重要的影响。

But he could still end up breakfasting on Swiss-government issue muesli because all six are accused of nicking around 45 million pounds they should have paid to FIFA.

不过他最后仍有可能沦为瑞士政府&议事餐桌&上的一道早餐，因为这所有六个人都被指控把本应支付给国际足联的大约4500万英镑骗了个精光。