一氧化氮

M. penetrans can stimulate the production of NO and inhibit mouse macrophages prolification, thus it may be an important etiological factor.

穿透支原体的脂质相关膜蛋白能诱导巨噬细胞产生一氧化氮，且能抑制巨噬细胞的增殖，因而可能与其致病性相关。

Objective To investigate the potential pathogenicity of Mycoplasma penetrans (M. penetrans), and to study nitric oxide production and cell prolification in lipid-associated membrane proteins-stimulated mouse macrophages.

目的 检测穿透支原体脂质相关膜蛋白能否诱导小鼠巨噬细胞产生一氧化氮及其对细胞增殖的影响，以了解穿透支原体潜在的致病性。

Thirdly, selective catalytic reduction of NO with propene over Cu ion-exchanged Al-MCM-41 has been studied.

在氮氧化物催化还原的活性评价实验中，考察了铜离子交换的Al-MCM-41对丙烯选择还原一氧化氮的催化活性。

We tested whether tetrahydrobiopterin (BH4) can recouple NOS and reverse preestablished advanced hypertrophy, fibrosis, and dysfunction.

我们检测四氢生物喋呤（BH4）是否能够使一氧化氮合酶恢复偶联并逆转心脏肥大、纤维化和功能障碍。

100 mice were randomly divided into high-dose group, middle dose group, low-dose group and the control group. The content of NO in liver, lung, cardiac muscle and skeletal muscle was detected after oral administration of different doses Tibetan Saussurea involucrata Kar. et Kir. water extract for treatment groups and saline water for control group in 24 days respectively.

方法］将100只小白鼠随机分为高剂量组、中剂量组、低剂量组和对照组，连续24 d分别灌服不同剂量的藏雪莲水提取物和生理盐水，然后处死小白鼠快速采取肝、肺、心肌和骨骼肌，测定各组织内一氧化氮的含量。

Methods Forty-eight healthy black and blue rabbits were chosen and divided into 6 groups at randomly. Experimental groups were performed surgery with CO2 laser sclerectomy in both eyes. Contents of nitric oxide and malonyldialohyed and the activity of Na(superscript +)-K(superscript +)-ATPase were tested with biochemical assays.

健康青紫兰兔48只，随机分为6组，实验组动物双眼均接受CO2激光外路巩膜切除术，化学比色法测定视网膜匀浆中一氧化氮、丙二醛的含量和Na-K-ATP酶的活性，进行统计学分析。

Methods The rat model of middle cerebral artery ischemia 2h/reperfusion 22h was established.The neurological scale, cerebral infracted volume, cerebral water content, activities of NOS and SOD were measured. ResultsThe average neurological scote,cerebral infracted volume,cerebral water content,activity of NOS,content of MDA in Naokangling Capsule group significantly decreased.

采用大鼠大脑中动脉缺血2 h/再灌注22 h模型，神经病学评分，脑梗塞范围及脑组织水含量变化，观察脑康灵胶囊抗脑缺血/再灌注损伤的效应；通过测定大鼠脑组织中一氧化氮合酶（nitric oxide synthase，NOS），超氧化物歧化酶（superoxide disumtase，SOD）活性及丙二醛（malondialdehyde，MDA）含量的变化以探讨药物作用的机制。

MethodsThe rat model of middle cerebral artery ischemia 2h/reperfusion 22h was established.The neurological scale, cerebral infracted volume, cerebral water content, activities of NOS and SOD were measured. ResultsThe average neurological scote,cerebral infracted volume,cerebral water content,activity of NOS,content of MDA in Naokangling Capsule group significantly decreased.

方法采用大鼠大脑中动脉缺血2 h/再灌注22 h模型，神经病学评分，脑梗塞范围及脑组织水含量变化，观察脑康灵胶囊抗脑缺血/再灌注损伤的效应；通过测定大鼠脑组织中一氧化氮合酶（nitric oxide synthase，NOS），超氧化物歧化酶（superoxide disumtase，SOD）活性及丙二醛（malondialdehyde，MDA）含量的变化以探讨药物作用的机制。

Methods:(1) Silica column chromatography and LH-20 were used in phytochemistry study.(2) Based on the extraction ratio of arctiin, the best extraction procedure was acheived under the direction of L9(34) orthogonal experimental design. From 10 types of resins, the one with the maximal binding ability for Arctii was selected and silica-column choramatograpy was used in its seperation and purification procedure.(3) The animal diabetic experimental model in Wistar rats was established with streptozotocin and many biochemical factors were used to indicate the pharmacological effects of arctiin, including glycosylated hemoglobin, serum creatinine, seralbumin, monoxide nitrogen, glycerin trilaurate, cholesterol, HDL-ch, LDL-ch, Blood urea nitrogen, creatinine, SOD, ET, MDA, total Urine protein and albuminuria.

(1)采用硅胶柱层析、聚酰胺层析、LH-20等方法对牛蒡子的化学成分进行研究；(2)采用正交实验设计方法，以牛蒡子苷为指标，确定了牛蒡子乙醇回流提取的最佳工艺；对10种类型大孔吸附树脂进行筛选，筛选出对牛蒡子苷吸附效果最佳的树脂；采用硅胶柱层析法，对牛蒡子苷进一步分离纯化；(3)建立实验性糖尿病大鼠模型，以GLU、TC、TG、HDL-ch、LDL-ch、TB、ALB、BUN、CREA、GHbAlc、NO、SOD、ET、MDA和尿液中TB、ALB为指标，研究牛蒡子苷药理活性；(4)以差速消化法纯化Wistar大鼠的主动脉内皮细胞并进行传代培养，测定牛蒡子苷对高糖条件下RAECs活性、RAECs释放LDH、MDA和NO的变化以及RAECs表达内皮型一氧化氮合酶表达的影响。

The inducible nitric oxide synthase is obviously expressed in speen of rats under the predator stress, suggesting important function for iNOS in speen physiology. However, the expression and subcellular localization for NOS in speen development and differentiation are largely unknown. In the present study, the changes in iNOS activity in spleen of rats under predator stress were studied to explore the role of iNOS in the decreased immunoactivity of the stressed rats. The changes in iNOS were observed with immunobistochemical method, and the contents of NOS and NO in spleen with biochemical method.

在捕食压力下，大鼠脾脏诱导型一氧化氮合酶明显表达，说明iNOS起到了重要的生理功能，然而，NOS的表达及在亚细胞水平的定位以及相应的差异变化等都很不明确，本试验采用捕食应激模型，利用免疫组织化学方法和生化测定，检测心理性应激对大鼠脾脏iNOS活性的影响及探讨这种影响与应激动物免疫力下降之间的关系。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。