一氧化氮

METHODS: A limb ischemia-reperfusion injury model in rats was established. The animals were randomly divided into three groups: control group, IR group and IPC group. The contents of diamide oxidase, nitric oxide, endothelin-1 (ET-1) and ratio of nitric oxide/endothelin-1 (NO/ET-1) in the plasma and the gut were measured. The leavels of myeloperoxidase, ratio of DNA chain, total nitric oxide synthase, inducible nitric oxide synthase and constitutive nitric oxide synthase in the gut were determined.

雄性Wistar大鼠18只，随机分为对照组，缺血再灌注组和缺血预适应组，每组6只，分别测定血浆和小肠组织二氨氧化酶、一氧化氮、内皮素-1(ET-1)、NO/ET-1比值的含量变化及小肠组织的髓过氧化物酶、DNA双链百分率(ratio of DNA chain %)、总一氧化氮合酶、诱导型一氧化氮合酶、结构型一氧化氮合酶的水平；免疫组化法检测小肠组织的诱导型一氧化氮合酶、内皮型一氧化氮合酶的表达。

Methods The DNA injury was determined with single cell gel electrophoresis, the contents of NO and NOS were measured by NO and NOS kits respectively and apoptosis of lymphocytes was determined by flow cytometry and gelose electrophoresis.

建立硫芥中毒淋巴细胞模型，分别用单细胞凝胶电泳法检测淋巴细胞DNA损伤；用镀铜镉还原法和一氧化氮合成酶试剂盒检测中毒后淋巴细胞培养上清液一氧化氮和一氧化氮合酶的变化；用流式细胞仪和琼脂糖凝胶电泳法检测细胞凋亡。

Although underlying mechanisms ⑴ hae not been elucidated, eidence points to the participation of the nitric oxide and cyclic guanosine 3',5'-monophosphate system.

虽然根本的机制还没有阐明，但是证据显示一氧化氮和环鸟苷酸系统参与其中。这里，我们检测到在MC3T3-E1成骨细胞的分化中基质金属蛋白酶13的mRNA，蛋白和活性的升高，诱生一氧化氮合酶和一氧化氮产物的升高。

It can promote the synthesis and release of NO and NOS and the expression of iNOS.

同时可促进一氧化氮、一氧化氮合酶的合成和释放（P.05；P.01）以及诱导型一氧化氮合酶mRNA的表达。

In addition, NOS activity decreased with the time prolonged in vitro, when oxyhemoglobin, a scavenger of NO, was applied, the NOS activity kept stable. This result above demonstrated that NO can inhibit NOS activity, and it was the inhibition of NOS activity by NO that may be an important protective mechanism to resist the neurotoxicity by Py.

另外，本实验还发现，在体外实验时，一氧化氮合酶活性可随时间的延长而逐渐下降，当向反应体系中加入氧合血红蛋白去除一氧化氮的作用后，则一氧化氮的抑制作用消失，酶活性保持稳定；以上结果表明一氧化氮对于一氧化氮合酶活性具有抑制作用，而且这种抑制作用，可能是机体对抗拟除虫菊酯毒性作用的一种重要保护机制。

The results showed that NO level and NOS activity was significantly increased in rat liver,kidney,heart and muscle after recurrent administration intraperitoneal of 50mg/kg cerous nitrate,at the same time,NO level was increased in rat liver and kidney after administration of 1mg/kg cerous nitrate.

结果显示，腹腔注射高浓度的稀土后，大鼠肝脏、心脏、肾脏、骨骼肌中一氧化氮、一氧化氮合成酶的水平明显增加，注射低浓度的稀土后，肝脏、肾脏内一氧化氮的量显著增加。

Study the activity of inducible nitric oxide synthase in the liver of rabbits infected with Eimeria stiedai, the concentration of nitric oxide in the serum、the coccidian oosysts per gram feces ,Score the pathologies according to anatomic structures of rabiits'livers and micoscopic pathological changes. So as to study the effect of exogenous NO and NOS of the rabbits infected with E.stiedai. The results showed that when the rabbits were infected with E.stiedai, the activity of iNOS in the liver and the concentration of NO in the serum gradually increased and they both reached the peak after 12 days in the control group , GTN and GSNO groups.

为了探讨外源性一氧化氮和一氧化氮合酶在家兔球虫感染时的作用，本试验以腹腔注射的途径给予感染斯氏艾美耳球虫的家兔NO供体硝酸甘油和亚硝基谷胱甘肽，对球虫感染家兔诱导型一氧化氮合酶活性、血清中NO的浓度、每克粪便的球虫卵囊数、肝的剖检和镜下病变及其病变计分进行了研究。

These sug- gested that this model has an excellent reproducibility and representativeness and is an ideal model for study of gunshot wound at maxillofacial region; Intensification of early management after damage of tissue can help achieve good curative effect.

对一氧化氮特殊生成途径—L-精氨酸/一氧化氮途径改变引起的NO生成量的变化与机体内多种生理和病理过程的关系的研究，使人们对这些过程的发生、发展的机制有了更加深入的认识；许多细胞因子也是通过对L-精氨酸/一氧化氮途径的影响而发挥调节作用。

However, because of the nonspecificity of the NOS inhibitors and the compensatory interactions among NOS isoforms, the ultimate roles of endogenous NO derived from the entire NOS system are still poorly understood.

然而，由于一氧化氮合酶抑制剂的非特异性以及一氧化氮合酶亚型(神经元型一氧化氮合酶，诱生型一氧化氮合酶，内皮型一氧化氮合酶)之间的相互代偿作用，研究人员对于体内由一氧化氮合酶系统合成的内源性一氧化氮的根本作用还不是很清楚。

Result The results indicated that the content of NO in liver, lung and skeletal muscle of high-dose group and in liver of middle dose group were significantly higher than that of control group and low-dose group (P.01). The content of NO in lung and skeletal muscle in middle dose group were higher than that of low-dose group and the control group (P.05). The content of NO in cardiac muscle in control group was higher than that of other group significantly (P.01). Tibetan Saussurea involucrata Kar.

结果］结果表明，高剂量组的肝、肺和骨骼肌和中剂量组的肝中一氧化氮含量中显著高于对照组和低剂量组，差异极显著(P.01)；中剂量组的肺和骨骼肌中一氧化氮含量高于对照和低剂量组，差异显著(P.05)；对照组的心肌中一氧化氮含量高于各药物组，差异极显著(P.01)。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。