一步法

Design another set of specific primers, of which upstream and downstream primers were located in the two sides of the cleavage site respectively, to develop the one-step RT-PCR method for detecting H5 and H9 subtypes of AIV. Identify the sensitivity of the developed one-step RT-PCR method by detecting 2 AIV strains of H5 subtype and 3 AIV strains of H9 subtype, and the specificity by detecting the RNAs of NDV, IBV and IBDV as well as analogue detection using computer software.

根据H5、H9亚型HA基因序列，分别设计一套特异性引物，其上、下游引物分别位於裂解位点两侧，应用RT-PCR一步法检测AIV H5和H9亚型，并验证所建立方法的敏感性；通过检测NDV、IBV和IBDV等RNA病毒及电脑软件模拟检测，验证其特异性。

One comprehensive processing process of coke oven gas and coking crude benzol was introduced in the paper, this process includes low and medium temperature

本文介绍了一种焦炉煤气和焦化粗苯综合加工工艺，此工艺包括低、中温加氢单元，变压吸附单元，CrystaSulf一步法硫回收单元和GT-BTX芳烃萃取蒸馏单元。

Methods A total RNA from ten cases of ovarian cancer and contral normal ovarian tissues were extracted by a single step method. The cDNA retro-transcribed from equal quantity mRNA derived from ten cases of ovarian carcinoma and normal ovarian tissues, which were labeled cDNA strand with Cy5 and Cy3 fluorescence as probes.

按一步法分别抽提10例人卵巢癌组织和对照正常卵巢组织的总RNA并纯化mRNA；分别从10例取等量的人卵巢癌组织和对照组织的mRNA分别混合后逆转录合成以Cy5和Cy3标记的cDNA一链做探针，混合后在含有8464点人类体细胞基因模板的表达谱cDNA芯片上进行杂交。

The one pace that has a design to produce per year 48 thousand tons is wet change filature to device, a classics technical reformation

现有一套设计年产4.8万吨的一步法湿法转向纺丝装置，一套经技术改造

TesTcard can be administered in one of two ways: as a single-step dip-and-read test or as a single-step pipette test.

TesTcard有两种操作方法：一步法浸入阅读测试或者一步吸取检测。

ShTat1.3 and ShTat1.5 are two variable antigen types of a cloning Anhui strain of Trypanosoma evansi.Total RNA of the two variable antigen types were extracted by acid guanidinium thiocyanate-phenol-chlorform single step method.

应用异硫氰酸胍一酚一氯仿一步法分离伊氏锥虫安徽株克隆虫体ShTatl的二个抗原变异体ShTat1.3和ShTat1.5总RNA，在含有甲醛的琼脂糖凝胶电泳后，转印到硝酸纤维膜上。

In the fourth chapter, an electrosynthesis from an iron wire gauze in KOH electrolyte is first presented for the in situ direct synthesis of the solid K_2FeO_4 with high purity by one step, and the techniques of FTIR, XRD, SEM, EDX are used to characterize the obtained sample.

本文第四章研究了一种经由一步法从KOH浓溶液中高效率低能耗直接电合成高纯度的固态K_2FeO_4的工艺方法，并使用富立叶红外、X射线衍射、扫描电镜及X射线能谱分析对所得样品进行了表征。

In this study,we examined the effects of a two-step culture system ,which use the different culture media,on early cleavage and competence of development of bovine zygotes in vitro fertilized.

结果表明，GSH与二步法培养系统结合相对于传统的一步法培养系统更适于牛体外受精卵的体外培养。

Ne-process two-for-one twister incorporating double twisting, setting and false twisting on one machine provides a new system for the production of silk-like crepe.

一步法倍捻机采用倍捻、定形、假捻3道工序在一台机器上一次完成，为生产仿真丝络线提供了一条新途径。

Liao source building glass products cover a number of types of building glass, the existing production lines, including two of the level of steel line, two lines of bending steel, a Low-E glass fiber reinforced plastic of line, two folders plastic glass production line, a hollow glass production line A GLAZE glass production lines, two bending glass production line, two one-step color folder folder wire glass production lines, three bilateral milling machine, automatic glass cutting machine Paul Quintero, many Units milling machine, positioning drilling machine, different line milling machine, Beveling machine, such as production equipment.

燎源建筑玻璃产品涵盖了多个种类的建筑用玻璃，现有生产线包括两条水平钢化线、两条弯钢化线、一条Low-E玻璃钢化线、两条夹胶玻璃生产线、一条中空玻璃生产线、一条彩釉玻生产线、两条热弯玻璃生产线、两台一步法彩夹夹丝玻璃生产线、三台双边磨边机、全自动保特罗玻璃切割机，多台磨边机、定位钻孔机、异行磨边机、斜边机等生产设备。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失