一步法

These have limited the development of furfural production. In this paper, one-stage process was reviewed briefly. Two-step process and current development of furfural production were discussed.

本文在对一步法的生产工艺进行概要介绍的基础上，重点对两步法糠醛生产工艺以及糠醛生产工艺的最新进展进行了讨论。

Application fields of sodium hexametaphosphate was summarized.Production technologies,mainly include two-step sodium dihydrogen phosphate production process and one-step phosphorus pentoxide production process of sodium hexametaphosphate,as well as advantages and disadvantages of various methods were introduced in detail.

综述了六偏磷酸钠的应用领域，并详细介绍了六偏磷酸钠的制备方法以及各种方法的优缺点，主要有磷酸二氢钠两步法和五氧化二磷一步法。

We compared the direct method of emulsigying sponge cake with the secondary method of emulsifying sponge cake .

试验对乳化海绵蛋糕制作的一步法与二步法进行了比较。

In the different process conditions, sugarcane wax was extracted from filter mud, a by-product of sugar mill, by two-step process and one-step process and can be used as the semifinished product for sugarcane wax refining and further processing.

以甘蔗糖厂副产品滤泥为原料，选定不同的工艺条件，分别以两步法和一步法提取蔗蜡，作为蔗蜡精制及深加工的中间产品。

Subsequently,with the straw and the cell freezing tube,we usedone-step and two-step method to vitrify the embryo of Lats gene knockout mice.The development ratio reached 90% 90.1 % 89.2%, 91.4% and the implantation rauo reached 50.0%, 47.1% 43.4%, 46.5% respectively after thaw.

在此基础上，用冷冻麦管、细胞冷冻管分别采用一步法、二步法对Lats基因敲除小鼠胚胎进行玻璃化冷冻，胚胎解冻后，囊胚发育率分别为：90％、90.1％、89.2％、91.4％；移植后着床率分别：50.0％、47.1％、43.4％、46.5％，差异不显著；并且与对照组相比差异也不显著。

The result indicated that, gathers in advance the emulsion water resistance and mechanics performance which in the body preparation method the one-stage process obtains is good, but two steps obtain the emulsion stability is good; The neutralizaing agent triethylamine amount used should enable to achieve neutral between x-y, to gathers in advance the body disperser and the emulsion stability only then is advantageous; The water affinity chain extendor DMPA content to the emulsion viscosity, stable and the emulsion cellophane water resistance has the remarkable influence, its amount used general control between x-y; Hard section isocyanogen diethylene glycol dinitrate primary control paint film degree of hardness, module, anti-avulsion, wear-resisting and so on performance, the soft section low-molecular polymer polyols primary control paint film elasticity, hydrolisis performance and so on bears the low temperature and bears

结果表明，预聚体制备方法中一步法得到的乳液的耐水性和力学性能较好，而两步法得到的乳液的稳定性好；中和剂三乙胺的用量应使中和度达到x-y之间，对预聚体的分散和乳液的稳定性才有利；亲水扩链剂DMPA的含量对乳液的粘度、稳定性及乳液胶膜的耐水性有着显著的影响，其用量一般控制在x-y之间；硬段异氰酸酯主要控制涂膜的硬度、模量、抗撕裂、耐磨等性能，软段低聚物多元醇主要控制涂膜的弹性、耐低温和耐水解等性能

In order to reduce the non-specific binding between oligonucleotides and bases mutation caused by the complicate secondary structure of DNA and excessive PCR amplification, a frequently phenomenon in one-step gene synthesis, we used a two-step method including assembly PCR and digestion-ligation step to synthesis Aspergillus niger lipase gene lipA.

本研究主要针对一步法长片段基因合成中复杂结构的非特异性配对和过多的PCR引入碱基错配等问题，采用二步法(组装PCR和酶切-酶连)合成了黑曲霉脂肪酶基因lipA。

In this thesis, PESA was synthesized using two different methods: the conventional one-step method and the two-step method we have developed. The corresponding products obtained by the one-step method and the two-step method are named PESA I and PESA II, respectively.

本文采用传统的一步法和非传统的两步法合成了PESA Ⅰ和PESA Ⅱ两种不同的产物，并通过单因素和正交试验确立了最佳合成路线。

The production technology of triethyl ortho - formate as medicine intermediate is reviewed.

综述了医药中间体原甲酸三乙酯的生产工艺，重点介绍了二步法、一步法、氢氰酸法、酯交换法和相转移催化法的合成原理与生产工艺，并对这几种生产工艺进行了评述。

Several methods applied widely for synthesizing uronic acid are introduced, such as methods of one-step process and two-step process.

分别就糖醛酸的几种常用合成方法进行了介绍，即直接将羟甲基氧化成羧基的一步法和先将羟甲基氧化成甲酰基、再氧化成羧基的两步法。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失