一步法

The catalyst conditions of 1,3-propanediol were researched, such as main catalyst, accelerant , solvent, etal.

针对一步法和二步法的技术路线，考察了不同催化条件对反应结果的影响，如主催化剂、膦配体、促进剂、活化剂和溶剂等。

In this paper, the catalyst and process of dimethyl ether from syngas were studied.

合成气一步法制二甲醚是近年来开发的一种新技术，相对于传统的两步法合成工艺，即先合成甲醇再脱水制二甲醚，合成气一步法合成二甲醚因其热力学上的有利性和经济上的合理性，成为C1化学研究的一个热点。

For household users to establish simple antifreeze dilution disposal ways of applying gender-controlled embryo freezingly-preserved technique by one-step vitrification dilution method,A,B,and C structure approaches of liquid layers in canaliculi were applied to investigate their survival capacity after embryos freshly-split by one-step dilution;and minimum capacity refrigerant method in specific canaliculus and above structure approach of liquid layer B were also applied to compare their survival capacity of frozen and melted embryos freshly split,and frozen and melted IVF embryos splited by one-step dilution.

为了创立在用户庭院就能应用的玻璃化保存性别控制胚胎的冷冻剂稀释处理方法，采用细管内3种不同液体层构成法，对新鲜分割胚一步法稀释后的存活能力进行了研究；其次用特定细管最小容量冷却法与上述3种不同液体层构成中效果较好的B法对玻璃化冷冻溶解的新鲜分割胚、体外受精分割胚及冷冻溶解的体外受精分割胚进行一步法稀释后的存活能力予以对比。

The flow sheet 图表 shown in FigⅠis intended to illustrate Ⅰ the one-step process.

是说明一步法的流程图。图①是说明一步法的流程图。

Soybean ; Bean pod mottle virus ; one step real-time fluorescent RT-PCR ; one step RT-PCR ; detection

大豆；菜豆荚斑驳病毒；一步法实时荧光RT-PCR ；一步法RT-PCR ；检测

All single step method and CTAB method did not work well when banana total RNA was isolated, unless modified single step method was used, in which, polysaccharide was removed by aether extraction prior to RNA precipitation.

对于富含多糖的香蕉果肉，一步法和CTAB异硫氰酸胍法的效果均不理想；改良后的一步法中增加了乙醚去除多糖并获得了较好的效果。

It has offered the information of the boiling points of the chemical compounds, and it is helpful to reutilize them after their separation by rectification. The results showed that the main compounds of the heavy cut of terpineol were longifolene and βcaryophyllene. They are from the raw material, terpinene oil.

松油醇的合成工艺方法主要有两种：一种是一步法，即松节油在酸催化作用下直接进行水合反应生成松油醇；另一种是二步法，即松节油首先在酸催化作用下水合成萜二醇，再经稀酸催化脱水生产松油醇。

Results Positive rate of Amsel was 28%, one step sialidase was 34%, Gram-stain was 30% and amines was 24%. Using Amsel as criterion, the sensitivity of one step sialidase and amines was 92.9% and 71.4%; specificity was 88.9% and 94.4%; positive predictive value was 76.5% and 83.3% negative predictive value was 97% and 89.5%, using Gram-stain method as criterion, the sensitivity of one step sialidase and amines was 93.8% and 66.7%, specificity was 92.1% and 94.3%, positive predictive value was 83.3% and 83.3%, negative predictive value was 97.2% and 86.8% respectively.

Amsel法阳性率28%，组织多胺试验阳性率24%，一步法唾液酸酶活性的检测试验阳性率34%，革兰染色细菌评分法阳性率30%；唾液酸酶活性检测法与Amsel法，革兰染色细菌评分法相比较差异无显著性；组织多胺试验与Amsel法，革兰染色细菌评分法相比较差异无显著性；以Amsel法为标准，一步法唾液酸酶活性检测法和组织多胺试验的灵敏度分别为92.9%和71.4%，特异性分别为88.9%和94.4%，阳性预期值分别为76.5%和83.3%，阴性预期值分别为97.0%和89.5%；以革兰染色细菌评分法为标准，一步法唾液酸酶活性检测法和组织多胺试验的灵敏度分别为93.8%和66.7%，特异性分别为92.1%和94.3%，阳性预期值都为83.3%，阴性预期值分别为97.2%和86.8%。

The best survuval rate of each stage thawed-embryos existed in groups of Zygote EDT3O-two-step method with I mm equilibration(66%), of Morulae EDT3O-two-step method with I mm equilibration(94%) and EDT4O-two--step method with 0.5 mm equilibration(94%), of Blastocyte EDT3O-two-step method with I mm equilibration(91%) and EDT4O-one-step method with I mm equilibration(91%).

总之，我们的研究表明，DET玻璃液冷冻方法是简单、经济而又高效的，冷冻小鼠各阶段胚胎都能获得较高的存活率，其中受精卵的EDT30二步法1分钟平衡组(66％)、桑椹胚的EDT30二步法1分钟平衡组(94％)和EDT40二步法0.5分钟平衡组(94％)、囊胚的EDT30二步法1分钟平衡组(91％)和EDT40一步法1分钟平衡组(91％)冷冻效果最好。

The synthesis of hyperbranched poly based upon 1,2-bis diallyl (methyl silyl ethane (B4) as a core molecule and diallyl silane (AB2) monomer via hydrosilylation addition have been investigated: And the hyperbranched poly were prepared using onestep without core, one-step with core and pseudo-one-step methods.

以四烯丙基硅烷(B4)为核、甲基氢二烯丙基硅烷(AB2)为单体，通过硅氢加成反应，采用无核一步法、核一步法、核多步法合成了超支化聚有机硅破烷。

As she looked at Warrington's manly face, and dark, melancholy eyes, she had settled in her mind that he must have been the victim of an unhappy attachment.

每逢看到沃林顿那刚毅的脸，那乌黑、忧郁的眼睛，她便会相信，他一定作过不幸的爱情的受害者。

Maybe they'll disappear into a pothole.

也许他们将在壶穴里消失