一次

And again, and again, and again...

一次一次又一次。。。

Move on to incline dumbbell presses. Make sure that every rep is a quality one; get a full stretch at the bottom of the movement and a strong contraction at the top.

重复上述动作，确保每一次重复都是高质量的，每一次移动到底部的时候都尽量伸展，每一次移动到顶部的时候都尽力收缩。

He missed his only shot, he turned the ball over once, but he did have one blocked shot.

是役，他唯一的一次尝试投篮失败，只有一次的传球，但同时也有一次的盖帽！

METHODS:The gene of human ET1 was synthesized according to the preferential codons of E. coli, cloned into the EcoRI and SalI sites of vector pThioHisA. The recombinant plasmid pThioHisA-ET1 was constructed , sequenced and transformed into E.coli TOP10. Induced and expressed fusion protein were identified and analysed by 12% SDS-PAGE and densitometry analyses. After the elution, denature and renature, the fusion protein Thioredoxin-ET1 was obtained by ProBondTM chromatogragraphy. The purity of Thioredoxin-ET1 was detected by HPLC. Inoculate Thioredoxin-ET1 once per mouse every 2 weeks in 25, 50 and 100μg separately on 3 groups for 4 times. 10 days after last inoculation, we obtained venipuncture blood.

根据人ET1的多肽序列合成ET1基因，将其插入到pThioHisA的EcoRI和 SalI位点，重组质粒pThioHisA-ET1进行酶切鉴定及序列测定验证后转化TOP10，IPTG诱导的重组菌经SDS-PAGE检测融合蛋白Thioredoxin-ET1的表达量；表达的融合蛋白用ProBond亲合层析纯化并经HPLC鉴测其纯度；每只小鼠按25、50、100ug/次剂量的Thioredoxin-ET1每两周免疫一次，共4次，最后一次免疫10d后制备抗血清，经Western blot和ELISA检测证明Thioredoxin-ET1融合蛋白具有ET1免疫反应原性。

To ensure success you must be willing to alter your course and keep altering it ,and to try again , as many times as it takes , to reach your destination .

确保成功，你必须愿意改变你的道路并且不断地更正，一次又一次地尝试，需要多少次就试多少次，从而达到你的目的地。

Everything was new to me and I was fairly new to that scene. When I first got into the game, I just wanted to dunk everything I could. That's the first opportunity to really show the world on an elite stage what you can do. It was the most unbelievable feeling and it just makes you want to keep getting that feeling over and over again.

我激动到不能入睡，对我来说一切都是新的，而且在我面前的是一个全新的场面，当我第一次投入到比赛中，我想尽我的全部所能扣篮，能是第一次向在世界面前展示自己所能做的，这是最不可思议的感觉，而且他使你想一次又一次的保持这样的感觉。

For example, retry="60 10 300 3" lets the consumer retry every 60 seconds for the first 10 times and then retry every 300 seconds for the next three times before stop retrying.

举个例子，retry=&60 10 300 3&就是将客户端在开始的10次里每60秒重试一次，然后在接下来的3次里每300秒重试一次，然后停止重试。

Yesterday marked the end of the most aggressive rate-cutting campaign in the modern history of the Federal Reserve; beginning in September, the central bank slashed rates at six straight meetings, and once between meetings, driving the rate from 5.25 to 2 percent.

去年9月，中央银行通过连续6次在利率会议上决定大幅将息，和一次在两次会议期间降息，使得美国利率从5.25%降到2％，开启了美国历史上最大的一次降息周期。

With advanced technology, Jeamd "container-hydrolock vertical-draining water-saving toilets" firstly create three-second dynamic super water-saving epoch concept, which is a revolutionary creation, with the super water-saving property of once water-using quantity: 1.4L /4L, revising the history of traditional toilets' once water-using quantity: 16L, 12L, 9L, or 3~6L.

佳美迪&容积水封式直排节水座便器&领先科技，首创3秒动力超节水时速概念，以一次用水量小档1.4升/次、大档4升/次的超节水特质，改写了传统座便器一次用水量16升、12升、9升、3-6升历史的革命首创；。

"Classic teaching in surgery has dictated that the use of analgesics should be withheld from children with acute abdominal pain until a surgeon establishes a definitive treatment plan," write Hannu Kokki, MD, from Kuopio University Hospital in Finland, and colleagues."It has been claimed that analgesia masks symptoms and physical findings, delays diagnosis, and leads to increased morbidity. Over the past few years, this traditional belief has been challenged."

孩童们被以随机的方式投予舌下oxycodone hydrochloride 0.1 mg/kg，或等量的生理食盐水；在第一次剂量投予前，1小时及3.5小时后，由同一位医师对参试者作身体检查、临时性诊断及临时性的病况判定；在第一次剂量的试验期中，每隔30分钟做一次疼痛指数的纪录，基线时也纪录一次，如此持续3.5小时；主要的效果指标，为疼痛程度的差异、有无腹部紧缩的状况及诊断的精确度等。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。