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The present invention relates to a molecular marker method linked with major QTL for cucumber downy mildew resistance and an application method, belonging to the field of biological technology.

本发明涉及的是一种分子生物技术领域的黄瓜霜霉病抗性主效QTL连锁的分子标记方法和应用方法。

The yield and purity of each DNA sample was tested by UV-spectrophotometer, the integrality of these DNA samples were run electrophoresis on the agarose gel. Each DNA sample was subjected to PCR amplification and hybridization using One lambada rSSO HLA-A,-B and -DRB1 commercial kit, the fluorescent intensity for positive bead and negative bead hybridized with HLA-A,-B and -DRB1 PCR products were calculated and analyzed.

使用2ml深孔板和TECAN DNA全自动工作站，从288份骨髓捐献者样本中提取基因组DNA；提取的DNA样本用紫外分光光度仪测定其浓度和纯度，并采用琼脂糖凝胶电泳检测DNA的完整性；DNA样本用One lambda rSSO HLA-A、B和DRB1基因分型试剂盒进行PCR扩增、分子杂交和Luminex流式磁珠分析仪检测，统计分析每一DNA样本HLA-A、B和DRB1基因扩增产物经DNA探针分子杂交后的阳性磁珠和阴性磁珠的荧光信号强度。

It was found that earlytransition metal monoxides ScO, TiO and VO do not form noble gas complexes, whilethe late transition metal monoxides coordinate one noble gas atom in forming thelinear NgMO(M=Cr, Mn, Fe, Co, Ni) complexes.

对于前过渡的ScO，TiO和VO分子，它们的9σ，1δ和4π价分子轨道能量很高，不能有效地与稀有气体原子形成络合物，而对于后过渡金属一氧化物则可以配位一个稀有气体原子形成直线型的NgMO(M=Cr，Mn，Fe，Co，Ni)络合物。

In this paper a new method has been proposed for computing the potential functions of internal rotations.

本文对分子内旋转问题作了以下的贡献：首先在理论上，提供了一种分子内旋转势函数的新的计算方法。

Transcription factors can control the transcription of their target genes when they bind to the DNA-binding sites on their promotors. They can transfer the messages of growth factors and so on to those effective molecules. Core binding factor al is such a kind of transcription factor, which can control the differentiation of osteoblasts peculiarly. They can induce the pro-osteoblasts or non-osteoblasts to synthesize and secrete most mineralize related proteins such as ALP, OC, OPN, BSP and so on, in order to differentiate to osteoblasts finally.

转录因子是一类可以通过与下游靶DNA结合，从而调控下游基因转录的分子，它们接受来自生长因子等方面的信号，再将这些指令具体地传递给直接执行功能的蛋白分子。cbfal是一种成骨分化特异性转录因子，可以诱导成骨前体细胞合成和分泌ALP、OC、OPN、BSP等矿化相关蛋白，向成骨细胞分化。

Many studies have been accomplished byvarious kinds of spectroscopic techniques with some important molecularparameters determined. There are, however, no wide-accepted molecular parameterstaken as a reference and no precise 〓 and 〓 obtained simultaneously so far.

人们运用各种光谱技术对NO在各波段的光谱进行了大量的研究，获得了一些重要的分子结构参数，但却没有一组公认准确统一的分子参数可供使用，也不能同时获得分子参数〓和〓。

Based on the traditional processing technology of bean curd film formation, a new type of automatic bean curd stick maker which employed the container conveyor made of glass fabric coated with polytetrafluoroethylene was developed.

0引言腐竹，又名腐皮，是蛋白质分子在变性过程中与多糖和脂肪通过分子间的相互作用而形成的具有多孔网络结构的可食性膜[1]，它是中国的一种传统大豆制品。

Construction of biologically functional bacterial plasmids in vitro . Proc. Natl. Acad. Sci. USA 70, 3240 – 3244 (1973).^ Patricia S. Thomas, Hybridization of Denatured RNA and Small DNA Fragments Transferred to Nitrocellulose PNAS 1980; 77: 5201-5205 PNAS Keith Roberts, Martin Raff, Bruce Alberts, Peter Walter, Julian Lewis and Alexander Johnson, Molecular Biology of the Cell

一个很典型的例子就是DNA分子大小的测量：在凝胶电泳出现之前，DNA分子大小是用蔗糖密度梯度沉降法来测量的，这一方法费时费力而且花费昂贵；而在梯度沉降法出现之前，使用的是黏度法。

Another conflict between palaeontologists and molecular biologists seems to have been similarly resolved. Since 1987 molecular biologists have believed on the basis of DNA evidence that modern humans— Homo sapiens —originated in Africa and then spread around the world.

古生物学家和分子生物学家之间的另一场争论似乎也得到类似的解决。1987年以来，分子生物学家一直坚信 DNA 得出的基本结论：现代人起源于非洲再遍及全球。

In addition,most of theprevious studies are focused on a very narrowly-separated doublets(e.g.,hyperfinestructure,Stark or Zeeman splittings)in a closed system.In fact,the atomic ormolecular system,especially the excited one,is an open system,but there are only afew studies for it.

另外，以往研究的多能级系统中相邻两能级的间距都很小(通常为原子的超精细结构、斯塔克分裂或塞曼分裂)，且大多数理论研究基本上都集中于封闭系统；然而，实际原子或分子特别是激发态的原子或分子体系通常为一开放系统，对于这方面的研究也很少。

The basic concept of FOP can be summarized as to further optimize effective prescription according to the standard of curative effects and with the aid of modern science and technology and theories of traditional Chinese medicine.

其基本内涵可概括为：以确有疗效的中药复方为研究对象，以现代科学技术和传统中医药理论为技术支持，以该复方所治病证的药效响应为评价标准，以优化重组疗效更优的新复方为研究目的。

Ever since our world has been a world, native forests have been indiscriminately exploited by man.

自从我们的世界一直是世界原生森林被任意剥削人。