查询词典 zebrafish

Full-length cDNA of Gibel carp pou2 gene was cloned from gastrula SMART cDNA library through RACE-PCR. It consists of 2421 bp, with an ORF of 1416 hp encoding a protein of 471 aa. The complete amino acid sequence shares 91.0% identity with zebrafish pou2 gene product.

用RACE-PCR方法从原肠期SMART文库中扩增到银鲫pou2基因的全长cDNA，其全长为2421bp，开放阅读框为1416bp，编码471个氨基酸，与斑马鱼pou2基因的氨基酸序列一致性高达91.0%。

At the one-cell stage, each embryo was injected with gradient doses of 2-8ng MO. The control morpholino, was purchased from Gene-Tools.To test the knockdown effectiveness of the morpholino, the tbx2-EGFP (enhanced green fluorescent protein) construct was generated. And coinjection of morpholino with mRNA tests its specificity of the morpholino. Overexpression of tbx2 via mRNA microinjection helps us understand gaining function of tbx2. Results The tbx2-EGFP construct tests the knockdown effectiveness of the morpholino. Zebrafish embryos lacking tbx2 function have defects in cardiac contractility, rhythm and morphology in a dose-dependent manner. The embryos with c-MO are normal in development. Coinjection of morpholino with mRNA rescue the phenotype of tbx2 knocking down. The phenotypes include pericardial edema, hypogenetic ventricle, dilation of atria, arrhythmia, bradycardia, asystole, abnormal atrioventricular canal, aberrant valve and blood regurgitation.

结果荧光蛋白融合标记实验验证了tbx2-MO可以很好得阻抑斑马鱼胚胎tbx2的表达，tbx2-MO组胚胎在心脏收缩性、节律和形态学发生上出现了异常，心脏畸形随着注射剂量的增加而出现比较一致的表型，而c-MO组胚胎没有出现明显的畸形；tbx2-mRNA和tbx2-MO的共注射减少了tbx2-MO胚胎心脏畸形的发生率及减轻了心脏畸形的程度，验证了所设计合成的tbx2-MO对tbx2基因的抑制作用具有特异性；tbx2-MO组胚胎心脏缺陷包括心室发育不良、心房扩张、房室管和瓣膜异常以及心率缓慢、心律不齐、心脏停搏、血液返流等，并根据心脏发育异常的程度分为轻、中、重度畸形组tbx2-mRNA基因过表达胚胎没有见到心腔的形成。

The use of retrovirus-mediated insertional mutagenesis in zebrafish has led to the mutation and rapid identification of hundreds of genes required for embryonic development and cell growth.

运用反转录病毒介导的插入突变技术，在脊椎动物斑马鱼中已经获得了许多影响胚胎发育和细胞生长过程的突变体，并找到了对应的基因。

The results of RT-PCR showed that zebrafish treated with TAA had adipogenetic and lipogenetic genes up-regulated on the third and 5th day, After injected pLF2.8-zfPPARγ 5-day postfertilization, treaded TAA 10 days.

由我们实验室发表的报告中，指出硫乙胺处理斑马鱼稚鱼七天，将造成脂肪肝炎。

The results of RT-PCR show that zebrafish treaded TAA 10th day made adipogenetic and lipogenetic up-regulated on the 3th and 5th day, because on TAA treated control Data show that lipogenetic gene would up-regulate at 9th day, so overexpression of PPARγ enhance TAA induced fatty liver.

反转录聚合链反应结果显示转殖PPARγ经TAA处理十天后，在第三有提高的现象，但是从硫乙胺处理控制组的结果显示第9天时，脂肪生成相关基因才会上升，故PPARγ的表现加强了硫乙胺诱导脂肪肝的形成过程。

Methods Eight STR primers were used to amplify DNA in the three outbred stock zebrafish.

方法利用5对斑马鱼STR引物和3对剑尾鱼STR引物，优化PCR条件，对不同种群斑马鱼DNA进行扩增。

The STR method is simple to operate,and it deserves to further study and lay the foundation for genetic mornitoring standard of outbred stock zebrafish.

结论鉴于该方法操作简便，通过进一步研究，可为封闭群斑马鱼的遗传质量评价打下基础。

Keywords acetochlor;heavy metals;joint toxicity;photobacterium phosphoreum;zebrafish embryos.

乙草胺；重金属；联合毒性；发光菌；斑马鱼胚胎。

Because of high reproductivity andtransparent embryos, zebrafish becomes the best vertebrate for mutagenesis.

由于具有产卵量大，胚胎透明易于观察等优点，斑马鱼成为脊椎动物中最好的诱变材料。

During somitogenesis, zebrafish FoxD3 is known of maintaining the somitic expression of myf5, and subsequently regulates myogenesis. FoxD5, another gene of the FoxD family, is also expressed in forming somites of the anterior PSM. However, the regulatory function of FoxD5 in somitogenesis is not clear.

虽然已知斑马鱼中FoxD3在体节发育时，能维持myf5在体节的表现并藉此参与於肌肉的发育；但FoxD转录因子家族中的另一员，FoxD5，在体节发育时也会表现在presomitic mesoderm前端生成中的体节处，而这个FoxD转录因子是否在体节发育的过程中扮演调控的角色目前仍不清楚。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。