查询词典 zebrafish

Zebrafish, androgenesis, female nucleus inactivation, UV, delayed fertilization

斑马鱼；雄核发育；雌核灭活；紫外线；延迟受精

Two gene fragments of DEN-1(GenBank accession number DQ886390)and DEN-2(GenBank accession number DQ886391),differentially expressed between the ocular skin tissues of normal and albino turbot,were isolated by mRNA differential display and sequenced.It was confirmed by RT-PCR method that the expression levels of both DEN-1 and DEN-2 were down-regulated in the ocular skin tissue of albino turbot.The sequence homology search of DEN-1 revealed high sequence homologies with the UGGT genes of zebrafish and cattle,and the Ugcgl1 gene of Norway rat.High sequence homologies of DEN-2 were observed with the eya4 gene(the eye absent homolog 4)from red jungle fowl,zebrafish,human,Norway rat,mouse and dog.

应用mRNA差异显示技术，对比研究正常与白化大菱鲆有眼侧皮肤组织，克隆差异表达基因，经测序，RT-PCR 验证，差异表达基因片段DEN-1(GenBank登录号：DQ886390)与DEN-2(GenBank登录号：DQ886391)均在白化大菱鲆有眼侧皮肤组织中下调表达；通过BLAST检索发现，DEN-1与GenBank中的斑马鱼和牛的类似尿苷二磷酸-葡萄糖：糖蛋白葡糖基转移酶基因、与挪威鼠类似尿苷二磷酸-葡萄糖：神经酰氨葡糖基转移酶1(Ugcgl1)基因有较高同源性；DEN-2 与原鸡、斑马鱼、人、挪威鼠、家鼠和狗的眼缺乏同源框4(eya4)基因的同源性均较高。

Objective: We try to elucidate the probable mechanism of midblastula transition in zebrafish through study the cyclin C gene which is expressed maternally and zygotically during zebrafish embryo development.

斑马鱼胚胎发育过程中cyclinC基因母型和合子型表达的初步研究目的：通过研究cyclin C基因在母型和合子型的表达研究，阐释其在中囊胚过渡时期的可能作用。

Part IIMorpholino-mediated translational inhibition and overexpression oftbx2 in zebrafish cardiogenesis Objective To elucidate the role of tbx2 in zebrafish cardiogenesis via morpholino-mediated translational inhibition and overexpression on the base of expression pattern of tbx2. Methods Gene-specific antisense morpholino oligonucleotides were designed to against ATG region of zebrafish tbx2 to knockdown endogenous tbx2 gene.

设计tbx2-MO和对照MO，在斑马鱼受精卵的单细胞期，进行不同浓度的显微注射，并通过荧光蛋白融合标记实验和tbx2-mRNA、tbx2-MO共注射拯救实验，验证tbx2-MO抑制tbx2基因表达的有效性和特异性；基因过表达是通过给斑马鱼受精卵注射体外合成的tbx2-mRNA，来研究tbx2基因功能获得对斑马鱼胚胎心脏发育的影响。

This site provides image materials on fish development, including time-lapse recordings illustrate the morphogenesis of zebrafish embryos, from early cleavage to organ rudiment formation, fish epidermal cell movies, 3-D films of zebrafish gastrulation, and much more.

该网站提供有关鱼类发育的图像材料，包括阐明斑马鱼胚胎形态发生（从早期卵裂到器官初期形成）的时间间隔录象、鱼类表皮细胞影片、斑马鱼原肠胚形成的三维影片，等等。

Based on the study on cytogenetics of zebrafish, the genomes were compared between zebrafish and human, and the zebrafish Hox gene clusters were mapped to its chromosomes.

本文在斑马鱼的细胞遗传学研究的基础上，对斑马鱼与人类基因组进行了比较研究，同时对斑马鱼Hox基因簇进行了染色体定位研究。

Objective To identify biochemical genetic markers of outbred stock zebrafish AB, LF, and ST to develop a new genetic monitoring method for different zebrafish group.

目的 通过对AB、LF、ST（short tail，本地短尾群）三个封闭群斑马鱼的研究比较，建立封闭群斑马鱼的遗传生化标记。

NaF can induce significant increase of micronucleus frequency in eripheral erythrocytes of Zebrafish. Neither transgenic CryIA cotton seed powder nor regular cotton seed powder has obvious effect on micronucleus frequency in eripheral erythrocytes of Zebrafish.

腹腔注射氟化钠可以诱导斑马鱼外周血红细胞微核率的显著升高；转CryIA基因抗虫棉棉籽粉粉和普通棉棉籽粉粉一样，对斑马鱼外周血红细胞微核率无明显影响。

The homology of the Myostatin sequences was analyzed among species. The results showed that the mature region DNA for Myostatin of Jinhua pig has the similarity of 100%% 97%, 95%, 95%, 93%, 93%, 93%, 89%, 90%, 89%, 87%, 87%, 86%, 86%, 86%, 85% and 76% compared with that of pig, horse, human, monkey, cow, hare, goat, sheep, house mouse, rat, goose, turkey, chicken, pigeon, Xiaoshan chicken, Fan duck and zebrafish respectively. The mature region DNA for Myostatin of Xiaoshan chicken has the identity of 99%, 97%, 93%, 92%, 92%, 87%, 86%, 86%, 86%, 86%, 85%, 85%, 85%, 84%, 84%, 83% and 74% compared with that of chicken, turkey, pigeon, goose. Fan duck, monkey, human, horse, pig, Jinhua pig, house mouse, rat, sheep, hare, goat, cow and zebrafish respectively. The mature region DNA for Myostatin of Fan duck has the homology of 91%, 92%, 92%, 90%, 95%, 84%, 84%, 85%, 85%, 85%, 83%, 81%, 81%, 83%, 81%, 82% and 73% compared with that of chicken, Xiaoshan chicken, turkey, pigeon, goose, monkey, human, horse, pig, Jinhua pig, house mouse, rat, sheep, hare, goat, cow and zebrafish respectively.

将测序结果与GenBank上其它物种的相应序列进行同源性分析，结果显示，金华猪Myostatin与哺乳动物猪、马、人、猴、牛、兔、山羊、绵羊、小家鼠和大鼠的同源性分别为100％、97％、95％、95％、93％、93％、93％、89％、90％和89％，与鸟类鹅、火鸡、鸡、鸽子、萧山鸡和番鸭的同源性分别为87％、87％、86％、86％、86％和85％，与鱼的同源性为76％；萧山鸡Myostatin与鸟类鸡、火鸡、鸽子、鹅和番鸭的同源性分别为99％、97％、93％、92％和92％，与哺乳动物猴、人、马、猪、金华猪、小家鼠、大鼠、绵羊、兔、山羊和牛的同源性分别为87％、86％、86％、86％、86％、85％、85％、85％、84％、84％和83％，与鱼的同源性为74％；番鸭Myostatin与鸟类鸡、火鸡、鸽子、鹅和萧山鸡的同源性分别为91％、92％、90％、95％和92％，与哺乳动物猴、人、马、猪、金华猪、小家鼠、大鼠、绵羊、兔、山羊和牛的同源性分别为84％、84％、85％、85％、85％、83％、81％、81％、83％、81％和82％，与鱼的同源性为73％。

It can be used as a versatile instrument for techniques like gel staining, western blotting, in site hybridisation on fixed tissue as well as for approaches on embyos(mouse, frog, zebrafish and many others).

它作为凝胶的技术用途广泛的器具使用，西方在固定的薄纱织品上的位置杂交和为在胚胎上的方法(老鼠、青蛙、 zebrafish 和许多其他)。

The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应，而且减少跟风的可能性。