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Gas chromatography was employed to quantify the output of metabolization,and it was found that the yield of diacetyl for the mutant UV-3 was 18.7 fold to the wild-type 10293,up to 1.045g·L~(-1).Acetoin and ethanol yields were decreased by 48.4%and 71.4%,respectively.The yield of acetate was increased by 34.6%.
利用气相色谱测定代谢产物量,分析诱变前后菌株代谢途径中碳源的流向,结果表明突变株UV-3的丁二酮产量提高18.7倍,达到1.045g/L,乙偶姻产量降低48.4%,乙醇产量降低71.4%,乙酸产量提高34.6%,且遗传性质稳定。
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During the intermissive fed-batch process, the feeding medium was added 10 times separately to make the total amount of C and N sources equal to those added in the batch process, and the yield of maduramicin was improved to 9645 μgmL^(-1), which is 15.7% higher than that obtained during the batch process. In the continuous fed-batch process, the maduramicin yield reaches 10328 μgmL^(-1), which is 6.0% higher than that obtained in the intermissive fed-batch process, and is 29.4% higher than that obtained in the batch process.
在间歇补料分批发酵工艺中,分10次补加培养基,使碳、氮源的总浓度与分批发酵相同,马杜霉素发酵单位提高到9645μgmL^(-1),比分批发酵提高了15.7%;在连续补料分批发酵工艺中,马杜霉素发酵单位可达10328μgmL^(-1),比间歇补料分批发酵提高了6.0%,比分批发酵提高了29.4%。
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The result indicated that: the amount of the excess HF formed direct ratio to the yield of phosphorus pentafluoride and the optimum amount of the excess HF used is about 35%; the trickling rate of oleum shouldnt make the temperature of hexafluorophosphoric acid exceed 25 ℃ and the amount of oleum is 110% to 125% that of stoichiometry so that the content of phosphorus pentafluoride can be achieved 90%; the time and temperature of heating hexafluorophosphoric acid influenced both the yield and the content of phosphorus pentafluoride, and the optimum time should be about 30 minutes and the proper temperature was 148 to 153℃; when alcohol used asrefrigerant, the content of phosphorus pentafluoride was 94.03%, and the content can be up to 97.38%, provided that using liquid nitrogen as refrigerant.
通过实验发现,氟化氢用量与五氟化磷的产率成正比,最佳过量百分率宜在35%左右;发烟硫酸的滴加速度不能使中间产物温度大于25℃,发烟硫酸用量大于理论量10%~25%时,得到的五氟化磷纯度高于90%;六氟磷酸的加热温度对五氟化磷的产率和纯度均有影响,宜在148~153℃的范围内,加热时间应在30分钟左右;用工业酒精做冷凝介质得到的五氟化磷纯度最高只能到达94.03%,使用液氮做冷凝介质可以使五氟化磷的纯度达97.38%以上。
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With sodium acetate as catalyst, inosine was esterified with acetic anhydride in the yield of 92% to give 2',3',5'-triacetyl inosine, which was treated with pyridine and phosphorus oxychloride to form a pyridinium solution, followed by performing an amination at 40℃ and 0.25~0.30 MPa to achieve adenosine in the yield of 67%.
肌苷在醋酸钠存在下与醋酸酐发生酯化反应以92%的收率得到2',3',5'-三乙酰肌苷,其化合物经吡啶和三氯氧磷处理形成吡啶盐溶液,未分离直接在40℃、0.25~0.30 MPa压力下进行氨解反应以67%产率制得腺苷。
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And the PLA/Chitosan/ACP barriers with additional 2 wt% chitosan still maintain 56.58 % antibacterial ability after eightieth day. The different composition of PLA/Chitosan/ACP composites was prepared by water washing solidification from NMP/water/lactic acid consolvent. After storing at room temperature for 48 hours and taking it out to obtain a shapable composites. To investigate the influence of ratio of composition with the shapable property of composites, it was analyzed by Rheometer, TGA, AFM, XRD. The result of the different APC content had different effect both water content and yield stress of composite materials. The yield stress decreased with ACP content while increases. It was derived that ions, released from ACP interact with amide groups of chitosan to form water-absorbing ionic clusters through chelating or electrostatic attraction.
另外,以聚乳酸/几丁聚醣作为基材并添加不同比例的非晶型磷酸钙,经过水洗固化后可得白色片状沉淀物,於室温下静置48小时后,复合材之体积呈现膨胀且具有可塑形之特性,以热重分析仪与流变仪分析样品之含水率与屈伏应力,结果显示不同ACP含量对复合材含水率与屈伏应力具有消长变化,而PLA/Chitosan/ACP之可塑程度与其所吸收之水份多寡有关,推测可能机制为ACP磷酸钙盐解离后之金属离子与几丁聚醣之胺基透过螯合作用或静电吸引之交互作用,形成具吸水性之离子团簇分散於疏水性之聚乳酸基材中。
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A screening and cultivation method for Bacillus thuringiensis of high-yield high temperature resisting protease includes the following steps: high-yield high temperature resisting protease Bacillus thuringiensis Bt140 is obtained through adopting casein substrate agar to identify flat primary screen and fermenting enzyme activity measuring secondary screening; Plackett-Burman design and response surface analytical method are adopted to obtain optimal fermentation zymogenic formula and fermentation condition for Bt140 quickly; finally, shaking fermentation level reaches to 918.56U/mL.
一种高产耐高温蛋白酶的苏云金芽孢杆菌筛选及培养方法,采用酪蛋白底物琼脂鉴定平板初筛与发酵酶活测定复筛的方法获得高产耐高温蛋白酶苏云金芽孢杆菌Bt140;采用Plackett-Burman设计与响应面分析法快速获得了Bt140最佳的发酵产酶配方与发酵条件,最终摇瓶发酵水平可达918.56U/mL。
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Filed experiments of 2001 showed that treatments with fungicide either 2 days before or 2 days post inoculation, tebuconazole reduced the disease index of the spikes by 80.32% and 70.64, as well as increased grain yield by 12.95% and 8.74% in comparison to the untreated control. Application of metconazole led to decreases of the disease index by 80.5% and 68.0% and increases of grain yield by 11.41% and 11.45% compared with the untreated plants. In 2002, with the serious occurrence of the head blight, tebuconazole reduced the disease index of the spikes by 76.47% and 78.67% and metconazole reduced the disease index by 82.06% and 81.89% in comparison to the untreated control.
田间药效测定结果表明:与对照相比,接种前2d喷药与接种后2d喷药,2001年,戊唑醇可使小麦赤霉病病情指数分别减少80.32%和70.64%,产量增加12.95%与8.74%;羟菌唑可使小麦赤霉病病情指数减少80.5%和68.0%,产量增加11.41%和11.45%;2002年,在小麦赤霉病严重发生的情况下,戊唑醇能使小麦赤霉病病情指数减少76.47%和78.67%,羟菌唑能使小麦赤霉病病情指数减少82.06%和81.89%,产量增加极显著。
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The factory produced high-yield PCB powerful hammer crusher, stable quality, noise, high yield, easy maintenance, especially applicable to the processing and production of high-speed rail road paving stone, expected to greatly improve the shape of the jaw crusher is expected to film With the shortcomings.
本厂生产的PCB强效高产锤式破碎机,质量稳定,噪音小,产量高,维修方便,特别适用于加工生产高速路及铁路铺路石料、出料形状大大改善了颚式破碎出料是片状的缺点。
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From the research result mentioned above, it was concluded that the selection of excellent single shoot tissue culture seedlings was an effective approach to guarantee fast growth and high yield of rattan plantation; the application of advance planting technique, such as controlling planting time, seedling number per group and spacing was very important for the purpose of minimizing unfavorable effect of rattan planting on upper trees, increasing cane yield and improving the level of economic gain; the determination of optimal harvest regime by adjusting the first harvest time, the lower limit of stem length for cutting as well as the harvest interval offered technical support to utilize resources reasonably and sufficiently and to enhance the economic benefit dramatically.
综合分析,通过组培快繁技术选择优质组培单芽藤是保证藤林速生丰产的有效途径;根据藤种的生物学特性,选择适宜的林地和上层林分,采取调控种植时间、丛栽株数和株行距等造林措施是减少棕榈藤间种对上层树种不利影响、有效增加原藤产量和提高林地增益水平的关键措施;而通过控制初始采收龄、起采茎长和采收间隔期,确定藤林最佳采收方式是促进资源充分利用、提高藤林的经济效益的有力保障。
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The result showed that the yields of spring wheat and naked oats were negatively related to the monthly average temperature and the sum of them; the yield was positively related to the monthly precipitation and their sum.; and the yield didn't have distinct relation to the monthly sunshine hours.
结果表明,武川小麦和莜麦的丰歉与生长季各月的平均气温及其总和呈负相关,与生长季各月降水量及生长季降水之和呈正相关,与日照时数关系不显著。
- 相关中文对照歌词
- Yield To Temptation
- 推荐网络例句
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She gently rebuff ed him, but agreed that they could be friends
她婉言拒绝了,但同意作为朋友相处。
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If in the penal farm, you were sure to be criticized.
要是在劳改农场,你等着挨绳子吧!
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Several theories about reigniting and extinguishing of the arc have been refered.
本文综合考虑了几种电弧重燃和熄灭理论。