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Mut+recombinants were selected by PCR and high yield Mut+ recombinant was picked out by double film immunoblotting.

PCR筛选出利用甲醇快速型的重组子后用双膜免疫杂交法筛选出表达量较高的重组菌株。

To obtain high cell density and high yield of MTG by Streptomyces hygroscopicus, the effects of exponential feeding of glucose and the addition of nitrogen source on MTG production were investigated.

在吸水链霉菌分批发酵研究的基础上，通过在菌体生长阶段指数流加葡萄糖，进行高细胞密度培养，获得了较高的菌体量；待菌体生长进入产酶期后，通过补加氮源，为产酶提供充足的氮源，其中通过流加蛋白质氮源，可以减少蛋白酶对成熟MTG的分解，促进产酶。

The results show the recovery yield of Boc-DPP latent pigment increases with fine dispersive stability, high polarity, and low molecular volume of solvent.

实验结果显示，随著溶剂对颜料分散的稳定性增加及其极性增加、分子体积较小可以有效地增加隐性颜料Boc-DPP的回收率。

Because the the dispersive stability and chemical modification is an important factor, this study emphasize on the issues of latent pigment recovery yield, product polarity, molecular volume, and dispersive stability in solvents.

由於溶剂对颜料分散之稳定度及化学改质反应是ㄧ重要因素，故本研究针对不同溶剂的极性及体积大小和其对颜料分散的稳定度对改质回收率的影响做ㄧ系统之讨论。

They aim to generate higher yields than equity investors normally receive - the Schroder Income Maximiser, for example, aims to give a yield of 7 per cent a year.

这种基金旨在产生高于股市投资者通常能够取得的收益——例如，Schroder Income Maximiser旨在提供7%的年收益。

Under the conditions of 456 W power output and l0min irradiation time, the quantities of reactants are malonic acid 13.Og and vanillin lOg, in pyridine solvent (1 6m1), the yield of reaction is 80%.

本实验在额定功率为456W，辐射时间为10min下，反应物投料量分别为丙二酸13.6g，香兰素10g在16ml溶剂中进行反应。

And its increase rate amounted to17%in comparison with single -cropping patterns gained from yield indexes reflect the relative number of luminous energy achieved by corn and soybean at their growth stages,and it is one ofscientific basis for the appraising the a...

根据产量指数比较所获得的各间作比例的6种光合份额值，反映了玉米和大豆在生育期分别获得的光能相对数量，是评价不同间作比例的玉米及大豆实际单产的科学依据之一。

Was considered by analysts to be at least a partial failure because of its low explosive yield, less than the equivalent of 1,000 tons of dynamite, which is much smaller than most nuclear blasts.

分析家认为，朝鲜于10月9日进行的核试验至少从局部来说是失败的，因其爆炸威力不高，低于1000吨炸药的爆炸当量，比大多数核爆炸要小得多。

Was considered by analysts to be at least a partial failure because of its low explosive yield, less than the equivalent of 1,000 tons of dynamite, which is much smaller than most nuclear blasts.

分析家认为朝鲜10月九号的试验至少是一个部分的失败，因为它的低爆炸当量，少于1000吨炸药的威力，远小于大多数核爆炸。

Was considered by analysts to be at least a partial failure because of its low explosive yield, less than the equivalent of 1,000 tons of dynamite, which is much smaller than most nuclear blasts.

分析家们认为，朝鲜10月9日的核试验至少局部失败，爆炸当量低，还不到1000吨炸药，比多数核爆炸当量少得多。

The shaping method of noncircular part and the tool holder's radial motion characters in noncircular turning process are discussed in detail in the thesis.

论文详细研究了非圆零件的成型方法和加工过程中刀架的径向运动规律。

I have not really liked him,I do not like his this kind of disposition.

我没有真的喜欢他，我不喜欢他的这种性格。