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The optimal shaking flask fermentation conditions were: initial pH 5.5,inoculum 15%,volume charge 135 ml in 250 ml flasks,and nitrogen source 10 ml mixed with 10 g/L yeast extract and 20 g/L tryptone.

摇瓶分批发酵工艺条件的最佳组合是:起始pH5.5,接种量15%,装液量135mL,氮源加入量为10mL含有10g/L酵母膏和20g/L蛋白胨的有机氮源。

Results MRS medium was more suitable for producing bacteriocin than other media. The maximum plantaricin production was obtained in modified MRS medium containing 10 g/L maltose and 10 g/L glucose, 10 g/L yeast extract, 10 g/L tryptone and 2g/L tri-ammonium citrate, 1 mL/L Tween80, 6 g/L K2HPO4 ?

最佳培养基为MRS培养基;优化后的培养基配方为葡萄糖10 g/L,麦芽糖10 g/L,酵母提取物10 g/L,蛋白胨10 g/L,柠檬酸三铵2 g/L,吐温80为1 mL/L,K2HPO4·3H2O 6 g/L,乙酸钠5 g/L,硫酸镁0.2 g/L,硫酸锰

The results showed that the optimal auto-induction medium is: tryptone 19.17 g/L, yeast extract 9.59 g/L, Na2HPO4 5.72 g/L, KH2PO4 5.48 g/L,(NH4)2SO4 2.66 g/L, NaCl 3.33 g/L, glycerol 2%, glucose 0.68 g/L, lactose 6.33 g/L, MgSO4

实验结果表明,最优培养基成分为蛋白胨19.17 g/L,酵母膏9.59 g/L, Na2HPO4 5.72 g/L, KH2PO4 5.48 g/L,(NH4)2SO4 2.66 g/L, NaCl 3.33 g/L,甘油2%,葡萄糖0.68 g/L,乳糖6.33 g/L, MgSO4

According to the nutritional needs of B.bifidum,based culture and stimulated factors were optimized by quadratic design.The optimum based culture was as following: yeast extract was 0.9%,tryptone was 0.3%,soya peptone was 0.3%,glucose was 2.0%,bifidus was 0.8%,growth factor was 10%.

针对两歧双歧杆菌的营养需要,采用正交试验设计优化增殖培养基,结果表明,酵母膏是影响短双歧杆菌的显著因素,最佳的优化培养基配比是:酵母膏0.9%,胰蛋白胨0.3%,大豆蛋白胨0.3%,葡萄糖2.0%,双歧因子0.8%,生长因子10%。

ColipET32/IL-11/BL(DE21 recombinant human interleukin-11 in the flask were studied by orthogonal experiment design,including the of culture medium(glucose,tryptone,yeast extract and pH),shaking speed,medium volume and inoculum.

为了探讨发酵条件对大肠杆菌表达人白细胞介素-11融合蛋白的影响,利用正交实验设计,对工程菌的生长条件和人白细胞介素-11融合蛋白表达进行优化。

Coli pET32/IL-11/BL(DE21 that expressed recombinant human interleukin-11 in the flask have been studied by orthogonal experiment design, including the components of culture medium (glucose, tryptone, yeast extract and pH), shaking speed, medium volume and inoculum concentration.

为了探讨发酵条件对大肠杆菌表达人白细胞介素-11融合蛋白的影响,利用正交实验设计,对影响工程菌的生长及重组人白细胞介素11融合蛋白的表达因素如培养基中的葡萄糖浓度、蛋白胨浓度、酵母提取物浓度、无机盐浓度、pH及摇床转速、装液量、接种量等在摇瓶中进行了初步的研究。

The estrogenic activities and mutation effects of nonylphenol ethoxylates before and after microbial degradation were studied with the recombinant gene yeast and SOS/Umu test.

作者中文名:吴伟;吴滟;瞿建宏摘要:采用重组基因酵母和SOS/Umu试验研究了壬基酚聚氧乙烯醚(NP10EO)经生物降解前后的雌激素活性和致突变活性。

To find the highly efficient biological treatment conditions was found the results showed that ultrasound treatment and after biological treatment Uncaria alkali content than the untreated materials have been significantly improved, and the yeast of fermentation to deal with the rhynchophylline is the highest yield.

结果表明,经超声处理和生物方法处理后钩藤碱含量较未经处理材料有了显著提高,其中以酵母发酵处理钩藤碱收率最高。

Our results suggest functions for seven previously uncharacterized yeast genes: KIN3 and YMR269W may be involved in biological processes related to cell growth and/or maintenance

我们的结果提示了7个以前未确定功能的酵母基因的功能:KIN3和YMR269W可能与细胞生长或维持的生物过程有关,而IES6, YEL008W, YEL033W, YHL029C, YMR010W和YMR031W-A可能有代谢的功能。

A study was done to isolate and identify yeasts from apple, which (15 samples) were collected from the North Andre Apple Juice Co. Ltd. The yeasts were isolated with unpasteurized samples in the media of glucose-yeast extract-tryptone media. The identification of yeasts were done with the physiological characteristics and the API 20C AUX system. The Candida spp., Kloeckere spp. and Saccharomyces spp.

利用酵母粉-葡萄糖琼脂培养基对15个批次苹果原料样本中的酵母菌进行分离,并用API20C AUX菌种鉴定系统进行鉴定,得到的25株酵母菌分别属於假丝酵母菌属、酿酒酵母菌属和克勒克酵母菌属的6个种:克柔/平常假丝酵母菌 13株,酿酒酵母菌 5株,克勒克酵母菌属酵母菌Kloeckera spp。

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