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Spherical yeast OS-194 is an only erythritol production osmophilic yeast. 10 percent of glucose ,0.5 g/dL for yeast extract and 0.1 g/dL for urea were the best components of medium,which obtained high erythritol yield by OS-194.The optimum culture conditions were 35℃,150 rpm,and 4 d.

球拟酵母OS-194是一株单产赤藓糖醇的耐高渗酵母,该菌株高产赤藓糖醇的最佳培养基配方为:葡萄糖10 g/dL,酵母膏0.5 g/dL,尿素0.1 g/dL。

RESULTS suc2 gene fragment of about 1.5 kb was amplified and cloned. DNA sequencing confirmed that , compared with the published sequence, the cloned suc2 gene had correct sequ ence except for one point mutation, but that did not alter the amino acid sequence o f t he encoded peptide. By transformation of yeast cells with a constructed gene dis ruption vector, four yeast clones were obtained which showed correct auxotrophy. PCR and DNA sequencing indicated that these yeast clones had the expected genot ype.

结果用 PCR从酵母基因组DNA中扩增出大小约1.5 kb的suc2基因片段,序列测定表明除585位有一点突变外,所得suc2基因与文献报道相同,构建的定位突变载体导入酵母细胞后,得到4株营养型符合的突变体,PCR表明这些突变体中均有suc2基因的同源重组,取其中1株的PCR产物进行序列测定证实了同源重组的发生。

As shown by the result, the biomass and astaxanthin content of oceanic red yeast was reduced due to the presence of β-ionone which inhibited the synthesis of carotenoids. The lethality rate of oceanic red yeast would attained 92.3% if 370 mg/L of the β-ionone concentration was contained in the plate medium. The oceanic red yeast mutated by ethyl methane sulfonate was cultured on plate medium containing 370 mg/L of the β-ionone. 200 mutants were randomly screened, positive mutants with increased the biomass, astaxanthin volumetric yield and astaxanthin specific yield accounted for 18% of the total strain, the mutants with higher biomass, astaxanthin volumetric yield and astaxanthin specific yield higher occupied 22.5%, 45% and 46%, respectively.

实验结果表明,在β-紫罗兰酮存在的情况下,由于类胡萝卜素合成受到抑制,海洋红酵母的生物量和虾青素合成量都减少;当平板培养基中β-紫罗兰酮浓度达到370 mg/L时,海洋红酵母的致死率为92.3%;在这种平板培养基上涂布经甲基磺酸乙酯诱变的海洋红酵母,随机筛选200个菌落,结果表明生物量、虾青素体积产率和细胞产率均有所提高的正突变株占18%,生物量、虾青素体积产率和细胞产率的单项指标有所提高的正突变株分别占22.5%、45%和46%。

The result showed that the fermentation ability and survival ratio of BY-03 and FY-03 remained at about 40% and 60%, which was obviously higher than commercial yeast after freezing and kept up at -20℃ for 4 weeks. BY-03 and FY-03's maltase ability were respectively 10. 12 and 15.10u/g, which were higher than commercial yeast, and BY-03 and FY-03's sugar tolerance were also better than commercial yeast under 0%-4% sugar.

实验结果表明,-20℃下冷冻4周后BY-03和FY-03的发酵力保持在40%以上,存活率在60%以上,明显高于市售普通酵母;其麦芽糖酶活力分别为10.12和15.10u/g,高于普通低糖酵母和普通高糖酵母;且在蔗糖质量分数为0%~40%的条件下生长良好。

We transformed antiapoptotic genes Bcl-2, CED-9 and PpBI-1 into the yeast to evaluate their function of resistance to cold stress. The results are as follows:(1) Cold induced apoptosis in yeast cells, moreover, yeast cells display representive features such as chromatin condensation, DNA fragmentation and chromatin margination.

而且酵母没有如Bcl-2家族的主要凋亡调控基因的同源基因,因此酵母是研究外源抗凋亡基因Bcl-2,CED-9和PpBI-1在抗冷方面的作用及其调控机制的理想体系。

If beer yeast was saved at low-temperature beer ,that maybe reduce yeast dead and yeast autolyze and could ensure the beer quality.

研究表明双乙酰还原结束后,应该低温储存酵母,以降低酵母的死亡率和酵母自溶,保证酿造的啤酒有纯正风味。

We selected the 2nd and the 3rd generation yeast with powerful vitality from the yeast mud fermented in tanks in working field as the ideal species source separated from yeast monoplast in beer and the ways to obtain them were as follows: 24 hours after deoxidisation of diacetyl to threshold value and decrease of its temperature to 5℃ was the best time for selection and sample-extraction location was at the nozzle of pressure-discharge pipes.

摘 要:从生产现场大罐发酵的酵母泥中选取第2~3代生命力强的酵母作为啤酒酵母单细胞分离的理想种源,选取的最好时间为双乙酰还原至阈值后降温至5℃,并让其停留24h时取样位置为排压酵母的管口,最后将所得菌种进行初选、复选或模拟生产恒温培养鉴定,选出优良菌株。

These yeast extracts may simply be labeled as 'Autolyzed Yeast Extracts' or as 'Yeast Extracts'.

这些酵母抽提物一般被简单标注为&自溶酵母抽提物&或&酵母抽提物&

There are two main categories of beer yeast: ale yeast and lager yeast.

啤酒酵母有两大类:淡啤酒酵母和贮藏啤酒酵母。

With this method, the effect of autolyzation on the yeast vitality and the variation of yeast viability when the beer yeast was stored in high or low temperature were studied.

通过这种方法,研究了啤酒酵母自溶对酵母细胞活性的影响,以及高低温贮存时酵母细胞活性的变化情况。

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