查询词典 yeast-bitten

Cloning of the Schwanniomyces occidentalis α-amylase and high expression in S.cerevisiae.The E.coli / yeast shuttle plasmid YCEpl partial library of Schwanniomyces occidentalis DNA was constructed and α-amylase gene fragments were screened in Saccharomyces cerevisiae by amylolytic activity.Several transformants with amylolysis were obtained and one of the fusion plasmids has about 5.0 kb inserted DNA fragment.It contains the upstream and downstream sequences of α-amylase gene from S.occidentalis .

以E.coli/yeast穿梭质粒YCEp1为载体构建西方许旺酵母部分基因组文库，在大肠杆菌中扩增后提取混合质粒DNA，经电转化非缺陷标志酒精酵母 AS.2.1364，在YPDS平板(含1%葡萄糖和1%可溶性淀粉)上用淀粉水解活力筛选含水解淀粉的阳性转化子，从阳性转化子中分离重组质粒证实含5.0kb的插入片段，用α-淀粉酶基因两端序列设计的引物PCR扩增及扩增片段序列分析证实该片段中含有α-淀粉酶全部编码序列。

To study the applicable prospect of suicide gene on tumor therapy in clinic,we cloned the gene of D-amino acid oxidase and the gene of yeast cytosine deaminase.We hope to establish the transgenic mices of DAAO gene and YCD gene,and the two transgenic strain mices are valuable animal system for studying the biological characteristic of DAAO gene and YCD gene ,for studing the killing activity of DAAO/D-Ala and YCD/5-FC gene therapy systems on tumor.The D-amino acid oxidase gene derived from R.gracilis and it could oxidize D-amino acids. Hydrogen peroxide(H2O2) is a reactive oxygen species generated in the deamination of D-Ala catalyzed by DAAO.

为了探讨自杀基因在临床肿瘤治疗中的应用前景，建立自杀基因肿瘤治疗评价的动物模型，我们克隆了D-氨基酸氧化酶(D-amino acid oxidase，DAAO)基因和酵母菌胞嘧啶脱氨酶(yeast cytosine deaminase，YCD)基因，希望建立DAAO基因及YCD基因转基因小鼠，为研究DAAO及YCD基因的生物学特性、开发和评价DAAO／D-Ala及YCD／5-FC自杀基因系统进行肿瘤治疗建立良好的实验动物模型。

Dissolve the yeast in warm water and let it stand until foamy, about 3 to 5 minutes.

将yeast 溶在温水中，直到很多小泡泡出来，大约3到5分钟。

Among the carbon sources examined, brown sugar and malt extract were best for the mycelial growth and the polysaccharide production. Besides, yeast extract and skim milk were the effective nitrogen sources.

在所测试的数种碳源中，以brown sugar和malt extract对菌丝生长及多醣生产效果较佳。yeast extract 和 skim milk为较佳之氮源。

A semivowel produced with the tongue near the palate (like the initial sound in the English word 'yeast').

舌头位于上腭附近发出的半元音（象英语单词"yeast"中的首音。

In model organism fission yeast,Schizosaccharomyces pombe,100-200 rDNA gene copies are located on both left and right ends of chromosomeⅢ.The integrity of rDNA is critical for cell growth and survival.

在模式生物裂殖酵母(fission yeast，Schizosaccharomyces pombe)基因组中，100-200拷贝的rDNA基因分布在Ⅲ号染色体左右两端的末端附近。rDNA区的完整性对于细胞生长和存活是至关重要的。

This strain, grown on Thermus medium containing tryptone and yeast extract at 55℃ could produce protease.

将此菌株在添加tryptone 和yeast extract 的Thermus 基本培养基中，於55℃下培养，会产生外泌性蛋白脢。

According to the above results, feather, tryptone, yeast extract as well as incubation time and temperature were selected for further studies by RSM. Data obtained showed the maximal biomass production and keratinase activity occurred at above 96 h.

根据上述结果选择feather、tryptone、yeast extract外加两个物理因子进行第一次的RSM分析，结果发现欲得到较高的角蛋白酶活性与菌体产量，其培养时间需超过四天以上。

Nine medium compositions were used to study their effect on I40 using Plackett-Burman design. The production of biomass for I40 was affected in order of yeast extract, tryptone, glucose, and production of protease affected in order of feather, tryptone and yeast extract.

最是化条件探讨方面，於基础培养基中分别添加九种单因子，以PB design分析，结果发现对於I40生长影响较为显著的因子依序为：yeast extract、tryptone、glucose，而对於该菌生产蛋白酶产量影响较为显著的因子依序为：feather、tryptone、yeast extract。

From the commercial consideration, the incubation time was fixed at 3 days and reinvestigated by RSM. The maximal keratinase activity was obtained from the following condition: tryptone 0.16%,yeast extract 0.27%, feather 0.08%, incubation temperature 51.7С.

由於长时间培养容易造成污染，且不具经济效应，因此将反应时间调整为3天，进行第二次的RSM分析，得到之培养条件为：tryptone 0.16 %， yeast extract 0.27 %， feather 0.08 %及 temperature 51.7С。

I didn't watch TV last night, because it .

昨晚我没有看电视，因为电视机坏了。

Since this year, in a lot of villages of Beijing, TV of elevator liquid crystal was removed.

今年以来，在北京的很多小区里，电梯液晶电视被撤了下来。