查询词典 xenografts

The function of EM65-2 on the RL95-2 was detected by in vivo and in vitro test. We established the protocol of making the human RL95-2 endometrial carcinoma xenografts in the ovariectomized nude mice at first. Secondly we detect the function of EM65-2 on the subcutaneous enografts with or without benzoate estradiol and estrone. The function was evaluated by the change of tumor volume and the weight of the tumor and the uterine at the end of the test.

VEGF的调控分也分为体内和体外两部分进行，体内部分利用前面实验中获得的不同条件下的裸鼠的皮下移植瘤和小鼠的子宫，通过免疫组化方法检测不同雌激素和抗雌激素的条件下的VEGF的表达情况；体外部分，首先建立RL95-2细胞的无血清培养方法，然后建立无雌激素正常pH、无雌激素低pH的培养液和雌二醇浓度为10nM的正常pH等三种条件培养液，于培养的不同时间分离上清液，通过Elisa法检测上清中VEGF的变化。

Dr. Farrar and colleagues found that in mice with tamoxifen-resistant human breast cancer xenografts, tamoxifen alone did little to alter tumor growth.

Farrar博士和他的同伴发现在移植了耐他莫西芬的人乳腺癌移植物的老鼠中，只用他莫西芬对肿瘤的生长起的改变很小。

Cobra venom factor was given to prevent hyperacute rejection and FK506 was given to prevent cellular rejection. The xenograft recipients were killed and cardiac xenografts harvested 4, 8, 12, 16, 24 hours after transplantation. Histologic examination was used to demostrate the degree of rejection and coagulation while revers transcriptase-polymerase chain reaction was used to detect the expression of tissue factor mRNA, and the guinea pigs hearts, harvested but not transplanted, were studied as controls in all samples.

建立豚鼠－大鼠异种异位心脏移植动物模型，使用CVF抑制超急性排异反应，FK506抑制细胞免疫排斥反应，分别在移植术后4、8、12、16、24h切取移植心脏，通过病理切片观察排异反应和凝血强度，同时通过RT-PCR方法检测组织因子mRNA表达强度，未移植豚鼠心脏作对照。

MethodsThe tumor xenografts model was established by injecting the mouse lymphocytic leukemia cells (P388) in the subcutis of anterior axillary of Kunming mice, and then was treated with CZBG by intragastric administration and different doses of adriamycin by intraperitoneal injection.

方法将小鼠淋巴细胞白血病细胞系P388细胞接种于昆明小鼠腋前皮下构建小鼠移植瘤模型，予复方浙贝颗粒联合不同剂量的阿霉素治疗。

Methods A model of nude mouse bladder tumor xenografts was established. As2O3--BDI-1 was poured into the bladder though urethra of female mouse with a trochar.

建立裸鼠膀胱肿瘤模型，经尿道置套管针膀胱灌注As2O3--BDI-1。

Get high purity DCs by Cultured plastic-adherent monocytes isolated from healthy human peripheral blood with GM-CSF and IL-4 for 7 days. To observe the morphology of DCs by inverted phase contrast microscope ,electron microscope and laser confocal microscope. Analyse phenotype of DCs with flow cytometry. Investigate the endocytosis ability of DCs as a group by Horseradish peroxidase endocytosis assay. To appraise allogeneic mixed lymphocytes reaction of DCs by MTT reduction assay. Analyse the levels of IL-12 and TNF in liquids of cultured medium by ELISA and MTT reduction assay respectively. Soluble antigens of HCCs was obtained by 3 freeze-and-thaw cycles. Biological characteristics of HC soluble antigens pulsed DCs were monitored by flow cytometry. According to MTT reduction assay estimated the cell proliferation of self lymphocytes activated by HC antigens pulsed DCs. Get high purity BCG HSP 70 protein by SDS-PAGE electrophoresis and determined its biological activity with ELISA. Analyse phenotype of antigen pulsed DCs primed by BCG HSP70 with flow cytometry. By MTT reduction assay estimated the cell proliferation of self lymphocytes and the MLR of DC based vaccine. Analyse expression of HLA-DR molecule on surface of HCC lines. The IFN-γ mRNA in lymphocytes after actived by DC vaccine and the Fas-L expression on DC and DC vaccine primed lymphocytes were detected by in situ hybridization and flow cytometry respectively. Specific cytotoxity lysis of T lymphocytes and nonspecific inhibition of liquids in culture medium against HCC lines were also tested. Detect expression of hAFP on four HCC lines with Cell-ELISA. Induce apoptosis of HCCs with actinomycin-D. Interaction of DCs and apoptotic cells was observed under transmission electron microscope. Growth inhibition test of DC against HCC lines was also performed. Establish the nude mouse model bearing human HC xenografts and indentify the characteristic of tumour by histochemistry and immunohistochemistry techniques. Prevent and treat transplanted human HC on nude mouse with Freezing and anabiotic HC specific lymphocytes.

用GM-CSF和IL-4从健康人外周血诱导DC；分别用倒置相差显微镜、电子显微镜及激光共聚焦显微镜观察DC形态；流式细胞术检测DC表型；HRP吞噬实验测定DC的群体内吞能力；MTT法检测同种异体混合淋巴细胞反应；ELISA法和MTT法分别测定DC培养上清液中IL-12和TNF水平；冻融法制备肝癌细胞可溶性抗原；流式细胞术检测负载肝癌可溶性抗原后DC的生物学特性；MTT法检测DC负载肝癌抗原后对自身淋巴细胞增殖的影响；SDS-PAGE制备电泳纯化BCG HSP70并鉴定纯度，ELISA测定活性；流式细胞术检测负载抗原DC经BCGHSP 70活化后的表型；MTT法检测肝癌DC疫苗对自身淋巴细胞增殖的影响和混合淋巴细胞反应；流式细胞术检测肝癌细胞表面HLA-DR表达；MTT法检测肝癌DC疫苗对自身淋巴细胞的活化；原位杂交法检测肝癌DC疫苗活化后的淋巴细胞IFN-γmRNA表达；流式细胞术检测DC和肝癌DC疫苗活化后淋巴细胞表面Fas-L；MTT法分别检测肝癌DC疫苗活化的淋巴细胞和其培养上清对肝癌细胞的特异性杀伤和非特异性抑制作用；Cell-ELISA检测人肝癌细胞hAFP表达；MTT法检测负载AFP表位肽和凋亡肝癌细胞DC对自身淋巴细胞增殖的影响；ELISA法和MTT法分别测定活化后淋巴细胞培养上清中TNF和IL-12水平；肝癌细胞凋亡的诱导和检测；DC吞噬凋亡肝癌细胞后的电子显微镜观察；DC对肝癌细胞的生长抑制试验；人肝癌裸鼠皮下移植瘤动物模型的建立及其组织学和免疫组织化学鉴定；DC及肝癌特异性淋巴细胞预防和治疗人肝癌裸鼠皮下移植瘤；冻存和复苏后的肝癌特异性淋巴细胞预防和治疗人肝癌裸鼠皮下移植瘤。

The xenografts were getting blacker gradually and aperistalsis of the graft occurred 15~ 20 min after the graft reperfusion.

再灌流后15-20min移植小肠发生超急性排斥反应，表现为移植小肠变黑，肠蠕动停止，失去活力。

In io studies were conducted with TRG xenografts in athymic mice.

很显然，该文中发现，胰腺癌细胞TRG，高度依赖HER2的存在。

Macrophages have been identified as the predominant cell within rejecting xenografts and were shown to be capable of destroying xenografts.

已有实验通过间接手段证实，巨噬细胞在异种移植的免疫反应中起效应细胞的作用，破坏移植物。

Result In control group, The tail moment in the four xenografts were significantly different (F=9.113, P＜0.01). The tail moment in the four xenografts exposed to irradiation (2Gy, 5Gy, 10Gy and 15Gy), reflected the descending tendency of radiosensitivity described below: lung adenocarcinoma＞neuroblastoma＞esophageal squamous carcinoma＞nasopharyngeal squamous carcinoma.

结果 未照射时4种人癌裸小鼠移植瘤的尾力矩有显著差异(F＝9.113，P＜0.01)。4种人癌裸小鼠移植瘤细胞在不同剂量(2Gy、5Gy、10Gy和15Gy)照射后，未做校正时的TM所反映放射敏感性由高到低的变化趋势依次为：肺腺癌＞神经母细胞瘤＞食管鳞癌＞鼻咽鳞癌。

What would he tell Judith and the children?

他将怎样告诉朱迪丝和孩子们呢？

I this is at that time, the opinion with peacockish true girl is full of in a heart.

这就是当时的我，一个心中布满虚荣的女孩子真实的想法。