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The oxygen uptake activity using Triton X- 100 as substrate in a resting cell experiment demonstrated that one of the isolates, P nitroreducens TX1, showed the highest activity to transform Triton X-lOO, 528 nmole/min/g cell wet weight among the isolates. The generation time of strain TX1 was only 1.2 h by using 0.5% of Triton X-100 as sole carbon source in MSB medium. Furthermore, this strain could even grow on the Triton X-l00 at a concentration of IO%-2O%.

本研究藉由测定各菌株分解TritonX-100时的氧气消耗活性发现,编号82.10.6之菌株,经鉴定并命名为P.nitroreducens TX1,每克湿重的菌株具有528 nmole /min的高耗氧活性,尤其,该菌株以0.5%的TritonX-100培养时,世代时间仅为1.2小时,即使在10%-20%的Triton-X-100的培养环境中亦能维持生长,在过去相关研究中均尚未发表有此一特殊之生长特性。

In addition, molecular orientation behaviors induced by shearing, electric field and magnetic field were investigated. The space group of the monomers A3EO7 and A9EO7 were determined as P2〓2〓2 and P112/m by means of electron diffraction and X-ray diffraction. Liquid crystalline properties of the monomer and the polymer were studied using DSC, variable X-ray diffraction, TEM and polarized optical microscope. Both monomers exhibit monotropic liquid crystalline behavior, and they also give a metastable phase on the cooling process. The polymer PA9EO7 shows enantiotropic liquid crystalline properties. Thus the rigid polymer backbone is not a fatal defect that completely distorts the packing arrangement of the mesogenic moiety in the side chain liquid crystalline polyacetylene.

结合电子衍射倾转方法和粉末X射线衍射技术,确定了两个液晶小分子A3EO7和A9EO7的晶胞参数和空间群,采用Cerius〓模拟软件,运用分子模拟的方法给出了小分子在晶胞中的排列方式;利用DSC、变温X射线衍射技术和透射电子显微镜研究了小分子和聚合物的液晶态,单体A3EO7和A9EO7表现出单向液晶性质,并且在降温过程中都表现出一个亚稳液晶相或亚稳结晶相,聚合物PA9EO7具有双向液晶性;采用偏光显微镜和X射线平板照相技术,研究了剪切和电场对聚合物分子取向的影响,在不同的液晶态,剪切作用对聚合物PA9EO7具有不同的诱导取向结果,直流电场可以诱导聚合物联苯液晶基元沿施加电场方向取向。

The biomechanical tests showed that two kinds of artificial bones had not significant difference on compressive strength and Young\'s modulus(P>0.05),while the flexural strength of nano-nacre artificial bone was less than the control group(P<0.05).3.The results of CCK-8 showed that the difference were not significant in each group,the proliferation of osteoblast reached the peak at the 5th day;7 days after being co-cultured,the total protein content of study group was higher than control group and blank group(P<0.05),while the difference between control group and blank group was not significantP>0.05The difference of alkaline phosphatase activities among three groups was not significant(P>0.05The SEM view showed that osteoblast attached and grew well in two kinds of artificial bone.4.X-ray photography showed that two kinds of powder started to degrade in 2 weeks;this phenomenon became more appear in 4 weeks,nano-nacre powder degraded faster than micron-nacre powder,while the hole shadow was easy to be found;in 8 weeks,all the femoral holes recovered and returned to normal bone mineral density in all groups.Analysis of tetracycline fluorescent double marks in the hard tissue grinding slices indicated that new bone grew fastest around the bone defect area in study group,while most slowly in blank groupP<0.05 SEM(scanning electron microscope observation showed that nano-nacre powder degraded more quickly.The same result can be found through the demineralized sections morphometric analysis,and both of the composite artificial bones made from those two kinds of nacre powder had the good connection with the adjacent tissue in rats body without apparent inflammatory response.5.X-ray photography showed that rabbit\'s bone defects healed faster in study group since NNAB implanted than in control group since MNAB implanted.At 24 weeks after operation,bone density in radial defects had nearly accessed to the normal area,while lower in control group,and turned up nonunion in blank group;The checking of BMD showed that results in study group were higher than those in control group at 8,16 and 24 week(P<0.05), and the difference between the BMD values in study group at 24 week and those in blank group was not significant(P>0.05).The gross specimens showed satisfactory histocompatibility both in study group and in control group,with bone tissue growing from two sides into the center of implanted materials; Normal slices in HE stain and hard tissue grinding slices in Stevenel\'s blue/Van Geison\'s picro-fuchsin stain showed that the bone growth tendency was better in study group than that in control group,and the medullary cavity had been penetrated to the implanted materials in study group at 24 week;Analysis of tetracycline fluorescent double marks in the hard tissue grinding slices indicated that new bone in both groups grew fastest 8 weeks after surgery,while slow down at 16 week.

纳米珍珠层/消旋聚乳酸复合人工骨与微米珍珠层/消旋聚乳酸复合人工骨分别与成骨细胞共培养后,其各时间点CCK-8法检测值与空白对照无显著差异(P>0.05),成骨细胞均在第5天达到增殖高峰期;培养7天后,实验组细胞蛋白含量高于对照组及空白组(P<0.05),后两者之间则无显著差异P>0.05碱性磷酸酶活性在三组间均无显著差异(P>0.05电镜下可见成骨细胞在两种人工骨上都有良好生长贴附能力。4.X-ray显示两种粉体在大鼠股骨骨洞植入第2周时都开始出现了降解,第4周时更为明显,纳米珍珠层粉较之微米珍珠层粉降解更快,而空白对照组骨洞阴影仍可见,至8周时,则所有组骨洞均己闭合修复,X-ray下已不可见原钻孔痕迹,恢复正常骨质密度;硬组织磨片四环素荧光双标记结果显示纳米珍珠层粉植入组较其余两组在骨缺损区周围新骨生长速度更快,空白组速度最慢P<0.05电镜观察及常规脱钙切片亦可见到纳米粉体降解较快;由以上两种原材料制得的纳米珍珠层/消旋聚乳酸复合人工骨与微米珍珠层/消旋聚乳酸复合人工骨在大鼠体内均与周围组织结合良好,无明显炎症反应。5.X-ray显示纳米珍珠层/消旋聚乳酸复合人工骨植入兔桡骨缺损区后其骨愈合速度较对照组微米珍珠层/消旋聚乳酸复合人工骨植入的快,至植入术后24周,实验组骨缺损区接近正常骨密度,对照组骨缺损区密度较低,空白组则呈现骨不连状态;骨密度测量结果显示术后8周、16周、24周实验组的骨密度值高于对照组(P<0.05,24周实验组的骨密度值与术前所测得的正常值无显著性差异P>0.05动物取材大体所见均显示组织相容性良好,骨组织逐渐由植入材料两端向中央生长;常规切片HE染色及硬组织磨片Stevenel\'s blue/Van Geison\'s picro-fuchsin联合染色均可见实验组骨缺损区长势优于对照组,至术后24周,实验组骨髓腔与材料已呈相交通状;硬组织磨片荧光显微镜下观察,两组材料在术后8周处于骨生长最快速时期,16周时速度开始减慢,术后4、8、16周时实验组的新骨生长速度均较对照组的快

Moreover, all the signals on X and Y only located in the heterochromatic regions, showing the same distribution with those of C-band.

所有X染色体上的信号是一致的,而X、Y染色体上的信号分布则彼此不同。X、Y染色体上的杂交信号区域均集中于异染色质区,信号分布模式同X、Y上异染色质分布模式完全吻合。

Process for the preparation of phosphatidylserine of formula wherein R and R independently represent a saturated, mono-unsaturated or polyunsaturated acyl C10-C30, X=OH or OM where M=alkaline or alkaline earth metal, ammonium, alkylammonium including the transphosphatidylation reaction between phosphatidylcholine of the general formula wherein R and R and X have the above specified meanings, R=CH2-CH2- NH2 o CH2-CH2-N(CH3)3 and Serine in D, L or racemic form catalysed by the phospholipase D enzyme, characterised in that said reaction is carried out in a hydroalcoholic medium containing an aliphatic alcohol and in the presence of bivalent metal oxide.

本发明涉及制备式CH 2 OR 1 CHOR 2 CH 2 O-P-OCH 2 -CH(NH 2 )-COOHX的磷脂酰丝氨酸的方法,其中R 1 和R 2 独立地表示饱和的、单不饱和的和/或多不饱和的酰基C 10 -C 30 ,X=OH或OM,其中M=碱或碱土金属、铵、烷基铵,该方法包括通式CH 2 OR 1 CHOR 2 CH 2 O-P-OR 3 X的磷脂酰胆碱其中R 1 和R 2 以及X具有上述特别的含义,R 3 =CH 2 -CH 2 -NH 2 或CH 2 -CH 2 -N (CH 3 3 与D、L或外消旋形式的丝氨酸之间的转磷脂酰化反应,该反应是通过磷脂酶D酶催化的,其特征在于所述的反应是在包含脂族醇的含水醇介质中并且在二价金属氧化物的存在下进行的。

Optimalerror estimates in L2-norm and H1-norm are obtained.Model:The mathematical model for two phase incompressible flow innaturally fractured reserviors is[34]:Supposeis a bounded domain with smoothy boundary,the rock ispresented byΩi,the volume of the fissures is small,Ωm=Ω.In the fissures Ω:s(x,0)=s0,x∈Ωin the rock Ωi:where J=0,T.

模型:裂缝孔隙介质中油水两相不可压缩驱动问题的数学模型为[34]:设〓为具有光滑边界的有界凸域,其中多孔岩块以Ωi表示,Ωm=∑Ωi,因裂缝体积很小,可以认为Ωm=Ω。x在裂缝系统Ω上:〓s(x,0)=s0,x∈Ω在任一岩块Ωi上:〓其中J=0,T。

In order to investigate the quantitative structure-retention relationship in gas chromatography, the molecular structure of monosubstituted alkane RX (X=halogen, OH, SH, NH2) is divided into two parts, R and X, to obtain molecular structure parameters, and the retention times in GC for 37 monosubstituted alkanes RX were determined.

将单取代烷烃RX(X=卤素,OH,SH,NH2等)分子结构分为两个区域来提取分子结构参数,从三方面影响因素(烷基R、取代基X、R与X的相互作用)来定量关联RX的气相色谱保留时间。

The phenomenon demonstrates that the perovskite is a stable state with low free energy. The (1-x) PMN- x PT system has a morphotropic phase boundary separating the tetragonal and rhombohedral phases. The MPB is located in the vicinity of x = 33%. The PMN-PT ceramics near the phase boundary possesses excellent piezoelectric performance. d33 can reach to 540 pC/N. kt and kp can be up to 0.45 and 0.62, respectively.

在准同型相界附近,PMN-PT陶瓷材料具有较好的压电性能,d33可达540 pC/N,kt、kp分别可达0.45和0.62;在准同型相界附近,极化后的(1-x)PMN-xPT陶瓷材料(0.22 x 0.35)的介电常数在温度诱导FR-FT相变过程中发生突变;PMN-PT陶瓷材料在FR-FT相变过程中呈现出一个热释电峰,反应了自发极化在相变过程中发生了非线性的变化。

Afterwards, the X-35C began a series of envelope-expansion flights and on 25 January 2001, the X-35C completed tanker qualification trials with a series of air-to-air refuelings behind an U.S. Air Force KC-10. The X-35C then completed its first supersonic flight on 31 January 2001 before being ferried from Edwards Air Force Base, California to Patuxent River Naval Air Station, Maryland.! t5 F* M* w. F.}% s

然后, X-35 C 开始了一系列的envelope-expansion飞行试验,并在2001年1月25日,在美国空军的KC-10 tanker耗尽的情况用air-to-air补给燃料试验飞行成功。2001年1月31日X-35 C在从爱德华空军基地被舰载到Patuxent 河海军加州马里兰空军基地后,完成了它的第一次超音速飞行。

The X-35C touched down at Patuxent River NAS on 10 February 2001, completing the first-ever transcontinental flight of a JSF demonstrator aircraft and initiating a series of flight tests that demonstrated carrier suitability in sea-level conditions. The X-35C's flight-test program included a series of Field Carrier Landing Practice tests to evaluate the aircraft's handling qualities and performance during carrier approaches and landings at an airfield, and also included up-and-away handling-quality tests and engine transients at varying speeds and altitudes

g2 X$ X5 Q6 ^2001年2月10日X-35 C抵达 Patuxent 河国家科学院,完成了JSF 型号机型的首次不间断横贯大陆的飞行试验,并且开始验证一系列在海上条件下同航母适配的的飞行测试。X-35 C系列试验科目包括一系列的Field Carrier Landing Practice以验证飞机在陆地机场起降的操作性能,也包括测试在起降操作性能上突然改变速度和高度产生的发动机的瞬时性能等。

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The split between the two groups can hardly be papered over.

这两个团体间的分歧难以掩饰。

This approach not only encourages a greater number of responses, but minimizes the likelihood of stale groupthink.

这种做法不仅鼓励了更多的反应,而且减少跟风的可能性。

The new PS20 solar power tower collected sunlight through mirrors known as "heliostats" to produce steam that is converted into electricity by a turbine in Sanlucar la Mayor, Spain, Wednesday.

聚光:照片上是建在西班牙桑路卡拉马尤城的一座新型PS20塔式太阳能电站。被称为&日光反射装置&的镜子将太阳光反射到主塔,然后用聚集的热量产生蒸汽进而通过涡轮机转化为电力