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Pronucleus expression recombinant pMAL-C2X-HBV-X is constructed successfully and with the IPTG induction, the recombinant pMAL-C2X-HBV-X can express the X protein in E. coli JM109, which lays the foundation fur the HBV X protein purification and its biological study.

成功构建了HBV X基因原核表达重组体pMAL-C2X-HBV X；在IPTG的诱导下，该重组体在大肠杆菌中可表达X融合蛋白，为进一步纯化HBV X蛋白和研究其生物学作用奠定了基础。

If X is a subclass of Y, then every instance of X is also an instance of Y. The rdfs:subClassOf relation is transitive. If X is a subclass of Y and Y a subclass of Z then X is a subclass of Z.

如果X是Y的一个子类，那么X的每个实例同时也都是Y的实例。rdfs：subClassOf关系是可传递的，即如果X是Y的一个子类，而Y又是Z的一个子类，那么X就是Z的一个子类。

If X is a subclass of Y, then every instance of X is also an instance of Y. The rdfs:subClassOf relation is transitive. If X is a subclass of Y and Y a subclass of Z then X isa subclass of Z.

如果X是Y的一个子类，那么X的每个实例同时也都是Y的实例。rdfs：subClassOf关系是可传递的，即如果X是Y的一个子类，而Y又是Z的一个子类，那么X就是Z的一个子类。

The results prove that T*1 S-closed space and T2 S-closed space are identical and that the regular S-closed space and normal S-closed space are the same. Therefore, to make T*1 space X become the complete conditions of S-closed space is the X extremely unconnected H-closed space, while S-closed space X can be measured as the complete condition X of S-closed T1 normal (A1) space.

首先讨论了S-闭空间的分离性，证明T*1型的S-闭空间与T2型S-闭空间是相同的，正则的S-闭空间与正规的S-闭空间是相同的，从而得到要使T*1型空间X成为S-闭空间的充要条件是X为极不连通的H-闭空间， S-闭空间X可度量化的充要条件是X为S-闭的T1型正则(A1)空间。

Abstract］ objective to improve the diagnosis of non-ossifying fiboma by analysing its x-ray feature.methods plain x-ray films was performed in all patients.x-ray finding in 8 cases with pathologically-confirmed non-ossifying were analyzed.results the clinical symptom was mild,the lesions occurred usually at the metaphysis of the long bones,the affected bones included femur（n=4）,tibia（n=3）,humerus（n=1）.cortical type was seen in 5 cases,presenting as unilocular or multilacular transparent areas with in the cortex or tightly beneath the cortex,the lesion had a sclerotic margin.which was more obvious at the marrow side.medullary type was seen in 2 cases.the lesion was located at the center of the bone and grew centrally.the tumor was manifested as unilocular or multilocular.tramsparent area with sclerotic border and the bone cortex became thinner with slightly expanding on 11 sides.conclusion plain radiography is the elementally means to detect this disease.based on the typical x-ray signs of non-ossifying fibroma combined with clinical data,correct preoperative diagnosis can be made in most cases.

目的 探讨非骨化性纤维瘤的x线表现以提高诊断水平。方法所有病例均摄有x线平片，分析经病理证实的8例非骨化性纤维瘤。结果临床症状轻，好发于下肢长骨干骺端或骨干，股骨4例，胫骨3例，肱骨1例。皮质型5例，表现为皮质内或紧贴皮质下的单层或多层透亮区，病变向骨内发展进入髓腔，周围有致密硬化带环绕，以髓腔侧明显；髓质型2例，病灶在骨内中央发展，显示为单房或多房透亮区，边缘有硬化，骨皮质菲薄，轻微向周围膨隆。结论 x线为最基本的检查方法，典型病例x线平片结合临床症状即可明确诊断。

Methods The whole-length X gene was directly cloned to pcDNA3.1 vector. The recombinant vector (pcDNA3.1-X) was transfected into HEPG2 cells after selection with hygromycin, steady clones (HEPG2-X cells) were obtained. The expression of RhoC protein was analysed with immunohistochemical stain.

用定向克隆的方法构建X基因的真核表达载体pcDNA3.1-X，脂质体转染HEPG2细胞；潮霉素选择培养稳定表达X基因的HEPG2-X细胞；免疫组化鉴定HEPG2-X细胞RhoC蛋白表达。

For fixed generalized reflection matrix P, i.e., P^T=P, P^2=I, then matrix X is said to be generalized bisymmetric, if $X=PXP$ and $X=X^T$.

对于某个广义反射阵P，满足P^T=P， P^2=I，那么称矩阵X是广义双对称的，如果满足X=PXP及X=X^T。

The proportions of Ⅰ~Ⅱgrade and Ⅲ grade were 73.8%、 83.1%; 45.7%、 65.2% between 92 samples of Urgur patients and 188 samples of han patients . Lymphy node metastasis in Uygur patients were greater than Han patients in Ⅰ~Ⅱgrade and Ⅲ grade (X~2=13.14, P.001; X~2=5.08, P.05).(3) The proportions of the expression of VEGF-AmRNA and its protein were 31.8%、 57.1%、64.3%, 27.3%、 53.6%、 59.5% in difference pathological grade in 92 samples, With increasing of the pathological grade,the VEGF-AmRNA and its protein expression are highly (X~2=6.26, P.05; X~2=6.21, P.05); The proportions of the positive expression of VEGF-AmRNA and its protein were 68.4

392例浸润性乳腺癌组织中VEGF-AmRNA和其蛋白的表达在不同组织学分级中其阳性率分别是31.8%、57.1%、64.3%，27.3%、53.6%、59.5%；二者在不同组织学分级中表达差异具有统计学意义(X~2=6.26，P.05；X~2=6.21，P.05)；在淋巴结转移组与非转移组中VEGF-AmRNA和其蛋白的表达阳性率的分别是68.4%、31.49，61.4%、31.4%，在淋巴结转移组VEGF-AmRNA和蛋白的明显高于淋巴结未转移组(X~2=11.96，P.001；X~2=7.79，P.05)；VEGF-AmRNA和其蛋白的表达

Using the zoom mechanism and a range of parfocal objectives (0.5 x, 1.0 x, 1.6 x, and 2.0 x), it is possible to go from examining the whole organism to the cellular level and back with no major adjustments.

使用放大功能和各种等焦面的物镜(0.5 x， 1.0 x， 1.6 x， and 2.0 x)，能够在检查整体微生物到细胞水平的时候，无需进行重大的调节。

objective according to 14 cases of patients with post-traumatic bronchial rupture of the ct and x-ray findings.analysis of its causes,trauma type,and ct,x-ray value and the advantages and disadvantages.methods 14 cases of bronchial rupture in patients with clinical data,x-ray,ct-chip analysis and summing up.results traumatic bronchial rupture study the performance of the main image①bronchial cut-off levy;②atelectasis and pulmonary fall levy③pleural effusion,pneumatosis;④mediastinum,subcutaneous emphysema;⑤many thoracic fractures;⑥traumatic wet lung,and so on.conclusion for traumatic bronchial rupture combined with x-ray and ct examination can be clearly diagnosed early.

目的 根据14例患者外伤后支气管断裂的ct和x线表现，分析其起因、外伤类型和ct、x线应用价值及优劣势。方法对14例支气管断裂患者的临床资料、x线平片、ct片进行分析、总结。结果外伤性支气管断裂的主要影像学表现①支气管截断征；②肺不张与肺坠落征；③胸腔积液、积气；④纵隔、皮下气肿；⑤胸廓多发骨折；⑥合并创伤性湿肺等。结论对于外伤性支气管断裂结合x线摄片和ct检查是可以早期明确诊断的。

By the time of its fall, most of the prisoners were writers who had written against the corruptions of the government.

到它被攻陷的时候，里面多数的犯人是写了反对政府贪污文章的作家。

The most obvious variation to ovum morphological character was that the color was changed from light green to sepiaceous in embryonic development, and all the ovums were almost hatched after 96h.

在胚胎发育过程中卵的形态特征最明显的变化是颜色从淡绿到深褐色，卵在发育96h后卵基本全部孵化。