查询词典 x-ray fish

After 10 days, 20 days and 30 days for grass carp (Ctenpharyngoden idellus Cuvier et Valencinnes) immunized by being injected Aeromonas hydrophilia, the ANAE (acid α-naphthyl acetate esterase) positive cells ratioes increased, and the same time it was found that the ACP activity increased up too, it showed that the fish bodies were being at immune responding situation and their immnue activities enhanced.

通过对草鱼免疫器官的六种同工酶的分析发现：LDH在血浆、血液细胞、脾脏和胸腺中都表达为5条区带，前肾还额外显示出X酶带，血浆只有3条酶带；ADH除了胸腺为一条带以外，其它的均为3条酶带；MDH和3-GPDH在这些组织中均表达为3条酶带；EST在前肾中为7条酶带，其余的都为6条酶带；POX只在前肾和脾脏中表达为2条酶带。

Fig 1 The results of two color FISH A:Normal sperms(a sperm nucleus with a red fluorescence domain was X sperm, and with a green fluorescence domian was Y sperm);B:Numeric chromosome abnormal sperm two green fluorescence domains within the same sperm nucleus which may be classified in Y disomic sperm or diploidy sperm, in the case of hybridization with two probes, one euchromosome specific and the other sex chromosome specific, this sperm could be discriminated as disomic or diploidy sperm

图1 双色荧光原位杂交结果 A：正常精子(精子头部有1个红色荧光信号者为X精子，有1个绿色荧光信号者为Y精子)；B：染色体数目异常精子两个绿色荧光信号同在1个精子核内，可为Y双体精子或二倍体精子，若用1条常染色体探针和1条性染色体探针进行双色荧光原位杂交，可以区别该精子属Y双体精子或二倍体精子

The different fishes which are employed in the present studies are wild-type salmon, cultured salmon of freshwater and seawater, sea perch and fat greenling. The complete CT gene sequences of salmons are obtained by PCR amplification. The partial CT sequences of sea perch and fat greenling are obtained by in vitro cloning PCR method. Alignment of obtained CT sequences with other fish CT shows that CT appears to be well conserved among the same family. And the relative in taxonomy is far away, the similarity of different fish CTs is low. On the contrary, the closer the relative in taxonomy is, the higher the similarity of different fish CTs is. The sCT is expressed in pGEX-4T-X by recombinant form . We also succeed in the research of sCT expression alone by expression PCR. In addition, sCT antiserum is obtained using GST-sCT as antigen, and the high titer is tested by double immunodiffusion. In the rat bioassay, administration of 50 μg recombinant protein evoked significant hypocalcemia at 1 h after the data are analyzed by t-test.

本文用PCR方法克隆了野生鲑鱼、养殖鲑鱼的降钙素基因，并应用体外克隆PCR的方法首次克隆出鲈鱼、六线鱼降钙素的部分基因序列，通过对克隆的降钙素序列的比较研究，结果显示同一科的鱼降钙素序列保守性较高，同时，根据降钙素的部分氨基酸序列进行了降钙素相似性的比较研究，结果显示在分类学上，分类地位较远的鱼，其降钙素相似性较低，分类地位越接近的鱼，其降钙素相似性越高；我们利用谷胱甘肽S-转移酶(Glutathions S-transferase，GST)融合表达载体pGEX-4T-X对克隆的鲑鱼降钙素基因进行了融合表达研究，应用表达PCR的方法对降钙素基因的独立表达进行了初步的研究探索，并将纯化的融合蛋白作为抗原，获得了高效价的兔抗鲑鱼降钙素免疫血清；生物活性研究表明，大肠杆菌表达的融合蛋白具有显著的降血钙作用。

The spiny eel , belonging to Perciformes, Osteichthyes, is a kind of little freshwater fish distributed in Southern China. In 1998, Our lab detected XX/XY differentiated sex chromosomes in this specie with karyotypic analysis, and then with chromosomal binding and SC observation, we identified that its two largest metacentric chromosomes were X, Y sex chromosomes in 1999. X, Y chromosomes differ each other not only in the distribution of heterochromatin but also in their shapes, which make them could be recognized directly under the light-microscope without any banding treatment.

刺鳅，属鲈形目、刺鳅科(Perciformes，Mastacembelidae)，是广泛分布于中国南方的小型淡水鱼类。1998年，我室通过核型分析，首次发现该物种具有明显分化的异形性染色体，其性别决定类型为XX／XY型。1999年，本室通过染色体显带、精母细胞联会复合体观察，再次确认刺鳅染色体组中最大的一对中部着丝粒染色体为X、Y性染色体，两者不仅已经出现结构异染色质分布上的不同，同时在外部形态上也存在明显差异，识别特征鲜明，在光镜观察下无需显带即能清晰辨认性染色体。

This is the first fish sex chromosome specific repetitive DNA sequence which was found in China so far.

将上述片段的探针对刺鳅染色体进行FISH分析后，结果显示，其中RMX6对于X染色体是特异的，其杂交信号仅限于X染色体上异染色质区。

Here we show that a newly evolved sex chromosome contains genes that contribute to speciation in threespine stickleback fish.

基因定位研究显示，新形成的X染色体包含雄性求偶行为基因，而先祖X染色体既包含行为隔离基因，又包含杂交种雄性不育基因。

To determine the true rate of aneuploidy and to elucidate the mechanism of aneuploidy,the sperms were decondensed for FISH analysis. It was found that the disomic frequencies of chromosome X was 0.208%. A new decondensation method was developed by trypsin treatment. The basic morphology of sperm was maintained.

为了解非整倍体的真实发生率及其发生机制，本文以X染色体α卫星DNA探针进一步检测出正常精子中X染色体的二体型频率为0.208％，同时，建立了一种保持精子基本形态的胰酶去凝聚的方法，为目前国内尚未开展的精子遗传物质结构的分子细胞遗传学研究，提供了新的手段。

When applied the spiny eel X chromosome library probes to the chromosomes of the two fish, different signals were distributed: in Siniperca chuatsi, besides some little signals on lot of autosomes, a large bright signal region was detected on the 8th chromosomes, which represented a synteny of spiny eel X chromosomes; but in Channa argus, no distinct synteny was found.

当以刺鳅的生物素标记X文库探针对鳜鱼和乌鳢的有丝分裂染色体进行染色体涂绘后，经过核型分析和统计分析表明，在这两种鱼的染色体上的杂交信号分布是不同的：在乌鳢中，除散在信号外，在其第八号染色体近着丝粒部位存在着一段较大的信号结合区域，应是与刺鳅的X染色体同源的保守同线群，而在鳜鱼中，只有散在信号分布于多条染色体上，没有明显的保守同线群存在。

If parents give their children a large number of unauthorized use of fish liver mooring, or injection of calcium and vitamin D and so on, can easily cause vitamin D overdose or poisoning; because these drugs are mainly used to treat rickets, and rickets in children caused by the teeth when the evening, his condition has become a very serious thing, if a square skull, ribs moniliforme, chicken, varus or valgus knees were "X" or O-shaped legs and so on.

如果家长擅自给孩子大量服用鱼肝泊，或注射钙剂和维生素D等，就很容易引起维生素D过量甚至中毒；因为这些药物主要是用来治疗佝偻病的，佝偻病引起小儿出牙晚时，病情已经很严重了，如出现方形颅、肋骨串珠、鸡胸、两膝内翻或外翻呈&X&或O形腿等。

A breeder using Rosetail bettas in their halfmoon lines has to be particularly careful of excess- fish who cannot swim properly, or who carry the trait into the mutation that has been coined ';X-Factor Fish';- fish with poor scales, shorter ventrals and collapsing finnage.

饲育者用玫瑰尾斗鱼培育半月斗鱼要非常小心，过度杂交可能产生无法正常游泳或突变的鱼。这些弊刁鱼通常体型小，腹鳍短，鱼鳍塌陷无力。

This one mode pays close attention to network credence foundation of the businessman very much.

这一模式非常关注商人的网络信用基础。

Cell morphology of bacterial ghost of Pasteurella multocida was observed by scanning electron microscopy and inactivation ratio was estimated by CFU analysi.

扫描电镜观察多杀性巴氏杆菌细菌幽灵和菌落形成单位评价遗传灭活率。