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Methods: M-CSF-dependent bone marrow cells were isolated from 5-6 weeks old mice, and cultured in the presence of MCSF (25μg/L) with different concentrations of TNF-α(0, 1, 10, 100 μg/L) for 5 days, the formation of TRAP multinucleated cells was observed. These cells were also cultured in the presence of both RANKL (30 μg/L) and M-CSF (25 μg/L) with or without 10 μg/L TNF-α for4, 5, 6 and 9 days. The number of TRAP multinucleated cells and resorption pits on dentine slices were counted under light microscope.

选用小鼠巨噬细胞集落刺激因子依赖性非附着性骨髓细胞，在含有25μg/L M-CSF和0，1，10，100μg/L TNF-α的α-MEM培养液中培养5d后，观察抗酒石酸酸性磷酸酶染色阳性多核细胞的形成；细胞在含有25μg/L M-CSF和30μg/L sRANKL的α-MEM培养液中进行培养，比较加入和不加入10μg/L TNF-α培养4、5、6和9d后，所形成的TRAP多核细胞的数目和骨吸收面积。

Results There was no significant difference in the number of HITS compared with patients with or without neuropsychological changes.

结果术中HITS的数值与术后神经心理学功能的是否有变化无关。

Results There was no significant difference in the number of HITS compared with patients with or without neuropsychological changes.

体外循环过程中HITS数值的变化与术后神经心理学功能变化无关。

"Although in our study the number of patients was small and follow-up short, we believe that HolCTU should be considered as an initial treatment for obliterative post-traumatic urethral strictures," the authors write."The procedure is simple, less traumatic, and less morbid, without a blood transfusion requirement, and the patient will require less time away from work. We do not deny that the core-through procedure has a slightly greater rate of failure compared with open surgery. However, failure of the procedure does not prevent conventional urethroplasty if required."

研究人员表示，虽然在研究中，受试者样本数少且追踪期短，但我们相信HolCTU将会像对创伤后闭锁性尿道狭窄的最初治疗般受到重视，因为这项手术过程简单、伤口较少，同时也较少导致其他病变、不需要输血，且患者较不需要花时间离开工作；我们不否定穿越核心的外科手术与一般外科手术相比，有较高的失败率，但无论如何，手术失败将不会阻碍传统的尿道成形术。

"Although in our study the number of patients was small and follow-up short, we believe that HolCTU should be considered as an initial treatment for obliterative post-traumatic urethral strictures," the authors write."The procedure is simple, less traumatic, and less morbid, without a blood transfusion requirement, and the patient will require less time away from work. We do not deny that the core-through procedure has a slightly greater rate of failure compared with open surgery. However, failure of the procedure does not prevent conventional urethroplasty if required."

研究人员表示，虽然在研究中，受试者样本数少且追踪期短，但我们相信HolCTU将会像对创伤后闭锁性尿道狭窄的最初治疗般受到重视，因为这项手术过程简单、伤口较少，同时也较少导致其它病变、不需要输血，且患者较不需要花时间离开工作；我们不否定穿越核心的外科手术与一般外科手术相比，有较高的失败率，但无论如何，手术失败将不会阻碍传统的尿道成形术。

The fatter mice in Gewirtz's study had been bred to lack a protein known as toll-like receptor 5 (TLR5), which most intestinal cells sprout on their surface. Its job is to recognize and bind to the whiplike flagella that bacteria use to move around. TLR5 acts as a traffic cop for controlling the mass of pathogens living in the intestine; without it, the normally harmless gut bacteria tend to overflourish and expand in number. See and listen to an audio slideshow about obesity rehab.

Gewirtz 实验中的胖小鼠普遍缺乏一种被称之为 toll 样受体5 (TLR5)的蛋白，这种蛋白是肠上皮细胞表面分泌的， TLR5的作用是识别并结合在肠道细菌的运动器官鞭毛上，在对大多数肠道病原体的控制中， TLR5扮演着交通警察的角色，没有它，一般无害的肠道菌就会异常活跃并且大量繁殖。

Even carbon number alkenes, phthalic esters and nitrogen heteroatom-containing components were detected in the elution fractions, while such components were not detected without silica gel column separation, indicating that preseparation is very important for increasing the sensitivity of GC/MS analysis.

各洗脱馏分用GC/MS分析，其可测成分与直接检测相比，多检测出偶碳烯烃、邻苯二甲酸酯类和含氮化合物等，表明合适的预分离对提高GC/MS分析的灵敏度非常重要。

With this,this paper suggests that to protect the endowment insurance interest of the migrant workers (especially the rural migrant workers),we execute an endowment insurance on a provincial basis without losing the urban mode,establish a system of permanent social security number,execute the system of social security document record,set up a central clearing and settlement system and branch it out provincially.

从这一新的价值选择出发，在保持现有各地实行的仿城型养老保险模式基础上，实行养老保险省级统筹；建立终身的社会保险号制度；实行社会保险档案记录管理制度；设立养老保险中央结算中心及其在各省的分支机构，以保障跨地区流动人员的养老保险权益。

Myelinated fibers ca n be seen without a myelin, the nerve axons silk increase in the number of accumulati on; Pulpless axon fibers can be seen swelling injury earlier in myelinated fibers.

有髓纤维可见髓鞘不整，轴突内神经丝数量增加，堆积；无髓纤维可见轴突肿胀，损伤要早于有髓纤维。

Objetive:To investigate the histopathological features and detect the mutation of JAK2V617F in PV、ET and IMF;to analyze the effects of the stages of myelofibrosis and the mutation on the number of leucocyte in PV and ET patients;Methods: the morphological features of 69 patients who dignosed in the first time were studied by HGF and Gomori stains ; the mutation of JAK2V617F was detected in 38 blood or marrow samples of PV、ET and IMF patients by real-time PCR;the effects of the stages of myelofibrosis and the mutation on leucocytes were analyzed;Results: Different stages of reticulin were observed in 15 PV and 15 ET patiens'marrow sections,and the cytosis、the pleomorphism and the bone trabecula side location of megakaryocytes were observed in all PV、ET and IMF patients;the means of leucocyte in patients with and without JAK2V617F were 20.4×109/Land 11.4×109/L respectively;the P value was 0.367 as comparing the leucocytes among different stages of reticulin increasing.

目的：探讨PV、ET、IMF患者的骨髓病理特点并且检测JAK2V617F突变的发生率，分析骨髓中纤维增生程度以及JAK2V617F对PV、ET患者白细胞数的影响；方法：69例初诊患者的骨髓切片经HGF染色以及Gomori染色后进行形态学研究；并利用real-time PCR法测38例患者的骨髓或者外周血的JAK2V617F突变；分析骨髓切片中纤维组织增生程度以及JAK2突变对患者白细胞数的影响；结果：15例PV和15例ET患者的骨髓切片中可以观察到不同程度的纤维组织增生，所有PV、ET、IMF患者骨髓病理中可见到巨核细胞增多、巨核细胞多形性改变以及巨核细胞定位于骨小梁旁；12例PV、4例ET、4例IMF中检测到JAK2V617F；有突变和无突变患者发病时白细胞数均值分别为20.4×109/L和11.4×109/L，比较不同纤维增生程度的PV、ET患者的白细胞数均值，P值为0.367。

According to the clear water experiment, aeration performance of the new equipment is good with high total oxygen transfer coefficient and oxygen utilization ratio.

曝气设备的动力效率在叶轮转速为120rpm～150rpm时取得最大值，此时氧利用率和充氧能力也具有较高值。

The environmental stability of that world - including its crushing pressures and icy darkness - means that some of its most famous inhabitants have survived for eons as evolutionary throwbacks, their bodies undergoing little change.

稳定的海底环境─包括能把人压扁的压力和冰冷的黑暗─意谓海底某些最知名的栖居生物已以演化返祖的样态活了万世，形体几无变化。