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Transcriptional activity of ACL recombinants normalized by Renilla was significant difference with pGL3-Basic expect for construct -27 and -15 (P＜0.01). Construct -919 contains highest activity. 3 times reduction of transcriptional activity from-919 to -679bp indicated that negative regulation factors located probably in this region. The activity started on construct -73 suggested the basal promoter activity was located within the -73 to +77bp region; Transcriptional activity of IDHβrecombinants was not significantly different between the recombinant -58 and pGL3-Basic. The activity started on construct -82, decreasing with the length of the fragment up to -164 in despite of a bit of fluctuation, and kept increasing from construct -164 up to -279. Thus, the basal promoter activity was located within the -82 to +16bp region, whereas the upstream 197bp conferred maximal transcriptional activity.

采用5'端缺失策略，结合启动子预测结果，分别构建了8个ACL基因和19个IDH3β基因启动子区重组子，将其转染猪PK15细胞系，并利用荧光素酶双报告基因系统检测了它们的活性，结果表明：在所构建的猪ACL基因5侧翼序列的8个重组子中，除pGL-ACL27和pGL-ACL15外，其它重组子的荧光素酶活性与阴性对照均达到极显著水平(P＜0.01)，表明它们均具有启动子活性，pGL-ACL919活性最强，到pGL-ACL679活性下降了将近3倍，说明从-919bp到-679bp区域可能存在负的调控位点，从pGL-ACL621到pGL-ACL73活性有小幅度下降，到pGL-ACL27活性降到与阴性对照水平无显著差异，表明维持该启动子的基本活性区域位于-73bp到+77bp之间；IDH3β基因启动子活性开始于pGL-IB82，然后虽然有些波动，但是活性一直下降直到-164bp处，之后活性又开始升高，直到-279bp处活性达到最高，这些表明维持该启动子的基本活性区域位于-82bp到+16bp之间，从-82到-279之间的179bp区域具有最高的启动子活性。

Results (1) From more than 100 primers, 11 primers producing polymorphism and reproduceable bands were selected, 129 bands were amplified.(2) The percentage of polymorphic bands within different populations were 20.9%-55.0%.(3) The cluster map including all samples were obtained by UPGMA. In the map, there were six cluster groups and three individuals outside the groups. The branch with five samples of Zhongjiang Sichuan population were far from other samples in genetic distance.(4) According to the distribution provinces five groups were divided in genetic variance analysis. Genetic variance 80.44% existing within population, 8.29% among populations and 11.27% among groups.

结果 （1）从100多个引物中选择出11个多态性强和重复性好的引物，扩增得到129条带；（2）不同居群内多态位点比率为20.9%－55.0%；（3）用UPGMA法得到所有样本的聚类图，可分为6个主要分支组和3个组外个体，其中四川中江居群的5个样本聚为一组，并与其他样本遗传距离较大；（4）按产地分组，遗传差异的80.44%存在于居群内，8.29%来自于组内居群间，11.27%的遗传差异来自于组间。

Whereas in glial cells, SP-induced calcium transients had fast kinetics with a rapid rise phase (0.99±0.25/s) and an exponential decaying phase (t〓=12. 6 s). In addition, the spike-like SP response had a steady duration and a distinct delay. SP receptors desensitized completely within a few minutes, and no resensitization was observed within an hour.

而且，SP在胶质细胞上引起的这种峰样〓变化具有稳定的时程，与给药开始时间之间有一个显著的延迟，并极易脱敏，几分钟内就可完全脱敏，1小时内没有发现复敏。

A revolution within the pattern, within the framework of society, is no revolution at all; it may be progressive or retrogressive, but like reform, it is only a modified continuation of what has been.

如果你反对改革，那你只能赞同革命；我们都知道一次世界大战之后的大革命已经证明是一场倒退的运动，就像我朋友指出的，各种恐怖和镇压的罪恶。

And(2) the effects of firm strength to the output level of companies within the industrial clusters.in this part of research,we use the same model built above,the only different is the change of parameter,samely, in this part of research, we study on changes of the output level of enterprises within the competition-ledding and cooperation-ledding industrial clusters when the gap between enterprise strength is different.

企业实力差距对产业集群内企业产出水平的影响研究这部分的研究中所构建的模型与上面的模型是同一个模型，只是参数的变化不同而以，同样在这部分的研究中，也是分别研究了在竞争主导型产业集群和合作主导型产业集群下集群内部企业的实力差距对企业产出水平的影响。

There were 10 seismometric stations （ including telemetric stations ） and 13 precursor monitoring stations with 17 observation items within the distance of 300 km from the epicenters. There were 18 medium term, short term and imminent anomalies. 7 of them were anomalies in seismic activity and 11 items were precursory anomalies. The proportion of precursory anomalies within the distance of 200 km from the epicenters was 73%.

在1994年度青海省震情研究报告中，根据青海东部存在的地震活动性异常和部分前兆观测异常，将共和地区列为应注意地区2）。2月16日5.8级地震前，根据地震前兆的短临异常，青海省地震局于2月12日填报了短临预报卡片（除共和地区外，同时还圈定了两个地区），并电话通知了共和县塘格木农场，14日向省政府做了汇报，省政府根据震情的发展用电报通知了海南州政府3）。

A selected one of the front mold or rear mold is axially and slidably movable within the bore relative to the other mold disposed within the bore to a desired one of the plurality of axial separation distances between the molds, whereby the volume is different for each separation distance.

所选择的前模或后模之一相对于设置在孔内的其他模具轴向滑动到一个模具之间所需的多个轴向间隔距离之一，由此每一间隔距离具有不同的容积。

There were no significant differences for the firing rates in the site of contralateral TNC neurons among during pre-CSD,CSD,and post-CSD (P＞0.05).For flunarizine group,the firing rates in the site of ipsilateral TNC neurons during pre-CSD were higher as compared with during CSD(P＜0.05).2.1 There were statistical differences on palasma levels of CGRP and SP among the three groups(P＜0.05).The levels of CGRP and SP in CSD group were higher than control group(P＜0.05).No significant differences on the levels of CGRP and SP in ipsilateral trigeminal ganglia were found among the three groups(P＞0.05).2 The number of neurons with positive CGRP and SP immunoreactivity was statistically different in right-sided trigeminal ganglia among the three groups (P＜0.05).The number in fight-sided trigeminal ganglia in CSD group was higher as compared with control group(P＜0.05).The number in right-sided trigeminal ganglia was statistically higher than that in left-sided trigeminal ganglion in CSD group(P＜0.05).3.1 Altered ReHo in ipsilateral pons and other brain regions response to pain such as basal nuclei,thalamus,cingulated gyms and prefrontal cortex was detected during the acute spontaneous attack as compared with during headache remission(P＜0.05,corrected by Monte Carlo simulation). 2 Positive functional connectivity was detected between ipsilateral pons and other brain regions related to pain within pain state and within non-pain state (P＜0.05,corrected by false discovery rate,FDR).Increased functional correlation between ipsilateral pons and other pain-related brain regions such as ipsilateral prefrontal cortex and contralateral subcallosal gyrus was detected during the acute spontaneous attack as compared with during headache remission(P＜0.05,corrected by Monte Carlo simulation).

结果1。对照组未发现CSD；同侧TNC放电频率，CSD中＞CSD后＞CSD前P＜0.05对侧TNC放电频率，CSD前、中、后无统计学差异(P＞0.05氟桂利嗪组同侧TNC放电频率，CSD前＞CSD中(P＜0.05)，CSD前与CSD后及CSD中与CSD后之间无统计学差异(P＞0.05)。2.1关于放免测定，各组血浆CGRP、SP水平有统计学差异(P＜0.05)，CSD组高于对照组(P＜0.05)，CSD组与氟桂利嗪组、对照组与氟桂利嗪组之间均无统计学差异P＞0.05各组之间同侧三叉神经节中CGRP、SP水平未见变化(P＞0.05.2关于免疫组化研究，右侧三叉神经节CGRP、SP免疫阳性细胞数三组之间有统计学差异(P＜0.05)，多重两两比较结果CSD组大于对照组(P＜0.05)，CSD组与氟桂利嗪组之间、对照组与氟桂利嗪组之间无统计学差异P＞0.05左侧三叉神经节CGRP、SP免疫阳性细胞数三组之间无统计学差异(P＞0.05CSD组中右侧三叉神经节CGRP、SP免疫反应阳性细胞数大于左侧(P＜0.05)。3.1局部一致性分析发现两组患者头痛疼痛状态较非疼痛状态脑活动发生变化的脑区有同侧脑桥以及其他疼痛相关脑区如基底节区、丘脑、扣带回、前额叶皮层等(P＜0.05，蒙特卡罗模拟校正)。2功能连接分析发现疼痛状态与非疼痛状态下主要疼痛相关脑区均与同侧脑桥有功能联系P＜0.05，false discovery rate，FDR校正疼痛状态与非疼痛状态比较，同侧前额叶皮层、对侧胼胝下回等疼痛相关脑区与同侧脑桥之间功能联系增强(P＜0.05，蒙特卡罗模拟校正。

In speaking of the subjectification of objectivity, what I have in mind is a conceptual reorientation through which talk of our access to objectivity is brought within or recast in terms of what can be found within subjectivity, understood as that domain to which we are held to have unproblematic access.

在说到objectivity 的subjectification，我想说的是'一个概念上的重定向'，通过它对objectivity的可达的说法是内部获得的，或是根据在subjectivity内部所能发现的东西而重铸recast的，而这个区域被理解为我们达到它是没有问题的。

Second, projecting subjectification conditions within pedagogical processes (e.g.,"invisible power/knowledge relation within curriculum reform" and "code of Teacher-Build and symbol significance").

其次，运用主体化系谱学方法，反省「课程改革过程中不可见的知识／权力关系」以及「从国家机器工具转化为学习伙伴：教师图像的符码与时代象徵」等问题。

However, as the name（read－only memory）implies, CD disks cannot be written onorchanged in any way.

然而，正如其名字所指出的那样，CD盘不能写，也不能用任何方式改变其内容。

Galvanizes steel pallet is mainly export which suits standard packing of European Union, the North America. galvanizes steel pallet is suitable to heavy rack. Pallet surface can design plate type, corrugated and the gap form, satisfies the different requirements.

镀锌钢托盘多用于出口，替代木托盘，免薰蒸，符合欧盟、北美各国对出口货物包装材料的法令要求；喷涂钢托盘适用于重载上货架之用，托盘表面根据需要制作成平板状、波纹状及间隔形式，满足不同的使用要求。