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The results showed that the optimized content of Mg~(2+) was 5mmol/L; dNTP was 200μmol/L; Primer was 0.5μmol/L; Taq polymerase was lU/50ul in the PCR system. Only one fragment of about 270bp was amplified within the genome of 12 wild species and 23 cultivars of Mallus Mill., which indicated that Ty1-copia-like retrotransposons were archaic components of apple genome. The nested insertion of near and within the reverse transcriptase gene of Ty1-copia-like retrotransposons wasn\'t found in apple genome.2 The reverse transcriptase conservative region of Ty1-copia-like retrotransposons were amplified from Gala apple using the optimized PCR system. The amplification product was isolated, cloned and sequenced. Forty-five clones containing reverse transcriptase conservative region were obtained (accession number: AY849580 -AY849592 and DQ105035-DQ105066). Cluster analysis of these sequences showed a great heterogeneity among RT domains isolated from the same genotype.

从苹果属12个野生种和苹果23个栽培品种中都扩增到了约270bp的目的产物，且所有试材的扩增结果一致，均无目的条带以外的产物扩增，说明Ty1-copia类逆转座子在苹果属植物中广泛存在，且存在历史可能较为久远；从扩增结果看不出该逆转座子的逆转录酶基因或附近区域在苹果基因组内有嵌套行为的发生。2、利用优化了的PCR方法从嘎拉苹果基因组克隆了45条Ty1-copia类逆转座子逆转录酶保守序列(登陆号分别为AY849580-AY849592和DQ105035-DQ105066)，结果表明，同一基因组来源的这些序列存在高度的异质性。

Sulfur isotope measurements of deposits such as those in the 1.1 Ga Duluth Complex, the Per-mo-Triassic intrusions of the Noril'sk area, and the 1.4 Ga Kabanga intrusions provide evidence for the derivation of S from both sulfide-and sulfate-bearing country rocks. The 1.3 Ga Voisey's Bay deposit provides an example where δ^34 S values of the ores commonly fall within the accepted mantle range of 0±2‰, but detailed studies of Proterozoic metasedimentary country rocks show that their weighted average δ^34 S value is also within this range.

如1.1 Ga的Duluth杂岩、Noril'sk地区二叠－三叠纪侵入岩和1.4 Ga的Kabanga侵入岩体中矿床的硫同位素测定结果证明硫来自含硫化物和硫酸盐的围岩。1.3 Ga的Voisbey's Bay矿床矿石的硫同位素δ^34 S值通常落入公认的地幔硫同位素组成范围(0±2‰)内，而对其元古宙变质沉积围岩进行的详细研究结果显示δ^34 S的平均值也在此范围。

Sulfur isotope measurements of deposits such as those in the 1.1 Ga Duluth Complex, the Per-mo-Triassic intrusions of the Noril'sk area, and the 1.4 Ga Kabanga intrusions provide evidence for the derivation of S from both sulfide-and sulfate-bearing country rocks. The 1.3 Ga Voisey's Bay deposit provides an example where δ^34 S values of the ores commonly fall within the accepted mantle range of 0±2‰, but detailed studies of Proterozoic metasedimentary country rocks show that their weighted average δ^34 S value is also within this range.

如1.1 Ga的Duluth杂岩、Noril'sk地区二迭－三迭纪侵入岩和1.4 Ga的Kabanga侵入岩体中矿床的硫同位素测定结果证明硫来自含硫化物和硫酸盐的围岩。1.3 Ga的Voisbey's Bay矿床矿石的硫同位素δ^34 S值通常落入公认的地幔硫同位素组成范围(0±2‰)内，而对其元古宙变质沉积围岩进行的详细研究结果显示δ^34 S的平均值也在此范围。

Sulfur isotope measurements of deposits such as those in the 1.1 Ga Duluth Complex, the Permo-Triassic intrusions of the Noril'sk area, and the 1.4 Ga Kabanga intrusions provide evidence for the derivation of S from both sulfide-and sulfate-bearing country rocks. The 1.3 Ga Voisey's Bay deposit provides an example where δ34S values of the ores commonly fall within the accepted mantle range of 0 ± 2‰, but detailed studies of Proterozoic metasedimentary country rocks show that their weighted average δ34S value is also within this range.

如1.1 Ga的Duluth杂岩、Noril'sk地区二叠-三叠纪侵入岩和1.4 Ga的Kabanga侵入岩体中矿床的硫同位素测定结果证明硫来自含硫化物和硫酸盐的围岩。1.3 Ga的Voisbey's Bay矿床矿石的硫同位素δ34S值通常落入公认的地幔硫同位素组成范围(0±2‰)内，而对其元古宙变质沉积围岩进行的详细研究结果显示δ34S的平均值也在此范围。

The probability of finding synchronously releasing colonies is much lower between species than within species. And in branch form Millepora, the probabiliy of finding synchronously releasing colonies is much lower between area than within area.

另外在同一地点的三种火珊瑚释出水母体的日期甚少相同，而且种间群体同步释放水母体的机率比种内低；在地点比较上，枝状火珊瑚在同一地点内的同步释放机率比在地点间的机率高。

Within these influences and modern nomocracy appeared such conflict as between manually governing tradition and nomocracy spirit、the nomothetical conflict within the traditional morality of legal system and nomocracy construction、the conflict between revolutionary tradition and the conservative spirit of nomocracy、between traditional awkward law consciousness and fine law requirement of nomocracy construction、between the law concept that regarding law as traditional tool and looking on law as justice and so on.

这些影响与现代法治建设之间产生了人治传统与法治精神之间的冲突、法制的传统道德与法治建设中的法律冲突、革命传统与法治的保守精神相冲突、传统的恶法意识与法治建设的良法要求相冲突、统治工具论法观念与公平正义法观念之间的冲突等。

According to the regression equations of initial crystallization temperature, the solubility of alumina and density at the temperature of 20 ℃ above its ICT of (2.23～3.0)NaF.AlF3-(0～5%)LiF-(0～15%)CaF2 system,the ICT isograms within the range of 910～950℃ and the density isograms at nonequal temperature within the range of 2.04～2.20g/cm3 under the 6%, 8%, 10%solubility of alumina were first obtained respectively.

基于(2.23～3.0)NaF.AlF3-(0～5%)LiF-(0～15%)CaF2系熔体初晶温度、变温氧化铝溶解能力和变温密度的研究，首次得到了氧化铝溶解能力为6%，8%和10%时温度范围在910～950℃的等溶初晶温度和密度在2.04～2.20g/cm3之间的等溶变温密度。

In view of an analysis from lithospheric geoid, horizontal stress and existing seismic tomographic data and so on, they show that Chinese mainland and adjacent area is controlled by mantle dynamics of interaction, mutual influence and constraint by western Pacific ocean plate, Indian plate and circum-Siberian arc tectonics that formed after Paleo-Asian ocean closed, which consist of three tectonic areas, with on the base of the Tethyan tectonic realm. Overall tectonic framework of the Chinese mainland is outwards obduction along main tectonic borders within shallow crust and inwards subduction and convergence within deep lithospheric mantle from south-western, south-eastern and northern parts, respectively.

基于岩石圈大地水准面异常、水平应力场和地震层析等地球物理资料综合分析可知，中国大陆及其毗邻地区在特提斯构造基础上，处于西太平洋板块、印度板块和古亚洲洋及其闭合之后环西伯利亚弧形等三大全球构造体系域相互作用、相互影响和相互制约的地幔动力学控制之下，总体大地构造格局是浅部地壳分别沿主要构造边界向外仰冲，深部岩石圈地幔则从西南、东南和北部向中国大陆俯冲会聚，使之整体处于地幔会聚挤压的大地构造背景。

The analyses of the meterological and oceanographical data from seriel observations aboard R/V Xiangyanghong 5 during the voyage of joint AMEX experiment by Australia,China and the United States show that (1) there is high level of humidity within middle and low layers of troposphere at the initial stage of a typhoon's formation,and once a typhoon has fully developed,the highest level of humidity remains within middle layer of tropisphere;(2) although small vertical wind shear is a requisition for a t...

对中、美、澳三国AMEX试验期间&向阳红5号&定点观测到的气象和海洋资料分析得出：①台风形成前，大气整个中、低层空气湿度较大，台风一旦形成，大气湿度最大层主要集中在中空；②风速垂直切变小，对形成台风是一个必要条件，但并非整个对流层风速切变都小，而且不同地区形成的台风，风速切变状况是不同的；③厄尔尼诺年南半球与北半球对应位置同期的θ_G、θ_8、Q_b相差不大，但是南半球的θ_c较北半球的大。

While a mating within a breed may be considered outbred, it still must be viewed as part of the whole genetic picture: a mating within an isolated, closely related, interbred population.

在同一犬种之内的交配可以被看作非近亲繁殖，它仍然被看作是整体基因图画的一部分：在一单一的，紧密相关的，杂交的种群内的交配。

In the negative and interrogative forms, of course, this is identical to the non-emphatic forms.

。但是，在否定句或疑问句里，这种带有"do"的方法表达的效果却没有什么强调的意思。

Go down on one's knees;kneel down

屈膝跪下。。。下跪祈祷